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Anti-clpX Polyclonal Antibody

Catalog #:   PXX04001 Specific References (22) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: G8X7L0
Overview

Catalog No.

PXX04001

Species reactivity

Flavobacterium columnare

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Flavobacterium columnare clpX (Met1-Ala163).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:2000-1:8000

Target

ATP-dependent Clp protease ATP-binding subunit ClpX,clpX,FCOL_04460

Purification

Purified by antigen affinity column.

Accession

G8X7L0

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with clpX antibody (PXX04001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
References

Hydrodynamic characterization of the FtsZ protein from Escherichia coli demonstrates the presence of linear and lateral trimers., PMID:39788364

CLPX regulates mitochondrial fatty acid β-oxidation in liver cells., PMID:37660922

The Fusaric Acid Derivative qy17 Inhibits Staphylococcus haemolyticus by Disrupting Biofilm Formation and the Stress Response via Altered Gene Expression., PMID:35369527

Degradation of the E. coli antitoxin MqsA by the proteolytic complex ClpXP is regulated by zinc occupancy and oxidation., PMID:34974059

Characterization of the Sinorhizobium meliloti HslUV and ClpXP Protease Systems in Free-Living and Symbiotic States., PMID:30670545

Chemical Cross-Linking Enables Drafting ClpXP Proximity Maps and Taking Snapshots of In Situ Interaction Networks., PMID:30415967

Deletions in guaBA and htrA but not clpX or rfaL constitute a live-attenuated vaccine strain of Salmonella Newport to protect against serogroup C2-C3Salmonella in mice., PMID:29927725

Mucosal immunization with caseinolytic protease X elicited cross-protective immunity against pneumococcal infection in mice., PMID:22728705

The chaperone ClpX stimulates expression of Staphylococcus aureus protein A by Rot dependent and independent pathways., PMID:20856878

Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD., PMID:20105222

Turnover of endogenous SsrA-tagged proteins mediated by ATP-dependent proteases in Escherichia coli., PMID:18550539

Distribution of the clpX gene in Brachyspira species and reactivity of recombinant Brachyspira pilosicoli ClpX with sera from mice and humans., PMID:17577058

Distinctive types of ATP-dependent Clp proteases in cyanobacteria., PMID:17371875

Analysis of the heat-adaptive response of psychrotrophic Bacillus weihenstephanensis., PMID:12382681

Antibodies against homologous microbial caseinolytic proteases P characterise primary biliary cirrhosis., PMID:11804659

Plant mitochondria contain proteolytic and regulatory subunits of the ATP-dependent Clp protease., PMID:11352464

The CtsR regulator of stress response is active as a dimer and specifically degraded in vivo at 37 degrees C., PMID:11069659

The clp proteases of Bacillus subtilis are directly involved in degradation of misfolded proteins., PMID:10809708

Conditional stability of the HemA protein (glutamyl-tRNA reductase) regulates heme biosynthesis in Salmonella typhimurium., PMID:9973348

HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome., PMID:8650174

The stroma of higher plant plastids contain ClpP and ClpC, functional homologs of Escherichia coli ClpP and ClpA: an archetypal two-component ATP-dependent protease., PMID:7580259

clpX encoding an alternative ATP-binding subunit of protease Ti (Clp) can be expressed independently from clpP in Escherichia coli., PMID:8093059

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-clpX Polyclonal Antibody [PXX04001]
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