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Anti-Phosphoprotein p30/Pret-105 Polyclonal Antibody

Catalog #:   PVV02501 Specific References (120) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P0C9Z3
Overview

Catalog No.

PVV02501

Species reactivity

African swine fever virus (ASFV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV Phosphoprotein p30/Pret-105 (Met1-Lys186).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:2000-1:8000

Target

Phosphoprotein p30,p30,Phosphoprotein p32,p32,Pret-105

Purification

Purified by antigen affinity column.

Accession

P0C9Z3

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with Phosphoprotein p30/Pret-105 antibody (PVV02501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
References

Lymphocytic Interstitial Pneumonia. PMID: 27514593

Pancreatic Cancer Metastases Harbor Evidence of Polyclonality. PMID: 26209539

Pre-analytics and tumor heterogeneity. PMID: 31580921

Clinical Considerations for Immunoparesis in Multiple Myeloma. PMID: 35565407

Annular Lichenoid Dermatitis (of Youth). PMID: 35076479

Intracranial pseudolymphoma. PMID: 10982160

Lymphocytoma cutis--case report. PMID: 24346899

Recombinant renewable polyclonal antibodies. PMID: 25530082

Editorial: The phantom menace: implications of polyclonality for surgical treatment of primary hyperparathyroidism. PMID: 29108697

The future of antibody therapy. PMID: 17824183

[Rheumatoid factors]. PMID: 6185910

Polyclonal staphylococcus endocarditis. PMID: 9243035

Clinicopathologic study of cutaneous plasmacytoma. PMID: 2242944

Mechanism of parathyroid tumourigenesis in uraemia. PMID: 10048455

Overview of the generation, validation, and application of phosphosite-specific antibodies. PMID: 21370022

The Evolutionary Landscape of Colorectal Tumorigenesis: Recent Paradigms, Models, and Hypotheses. PMID: 36702361

A Rare Case Report of Periorbital Pseudolymphoma. PMID: 37073202

Cytogenetic changes in the progression of lymphoma. PMID: 9720711

Comparison of in vitro and in vivo mitogenic and polyclonal antibody and autoantibody responses to peptidoglycan, LPS, protein A, PWM, PHA and Con A in normal and autoimmune mice. PMID: 3886911

Antibiotic-resistant infections in the critically ill adult. PMID: 15177843

Mitochondrial Metabolism in Pancreatic Ductal Adenocarcinoma: From Mechanism-Based Perspectives to Therapy. PMID: 36831413

tHapMix: simulating tumour samples through haplotype mixtures. PMID: 27605106

Recombinant human polyclonal antibodies: A new class of therapeutic antibodies against viral infections. PMID: 16787244

Many different tumor types have polyclonal tumor origin: evidence and implications. PMID: 18614394

Towards a comprehensive view of immunoglobulin class switching. PMID: 8442856

Development of recombinant human polyclonal antibodies for the treatment of complex human diseases. PMID: 16918257

Antibodies in infectious diseases: polyclonals, monoclonals and niche biotechnology. PMID: 21473942

Clonal diversity in carcinomas: its implications for tumour progression and the contribution made to it by epithelial-mesenchymal transitions. PMID: 18071912

Expansion of a mutated clone: from stem cell to tumour. PMID: 17468295

Polyclonality of Parathyroid Tumors in Neonatal Severe Hyperparathyroidism. PMID: 25828954

[A case report of cutaneous plasmacytosis]. PMID: 30122785

Activation of human B lymphocytes (the in vitro polyclonal B cell activator-induced plaque-forming cell system). PMID: 397602

Detection of cytokine mRNA in polyclonally-, antigen- or allergen-stimulated mononuclear cells. PMID: 2045118

How can the cellular immune response control hepatitis B virus replication? PMID: 10971819

Pseudolymphoma versus lymphoma: An important diagnostic decision. PMID: 27601833

Cytogenetic polyclonality in tumors of the breast. PMID: 9140449

Persistent hematopoietic polyclonality after lentivirus-mediated gene therapy for Fabry disease. PMID: 36816757

Ocular adnexal lymphoid neoplasms: clinical, histopathologic, electron microscopic, and immunologic characteristics. PMID: 7042523

Immunotherapy in the poisoned patient. Overview of present applications and future trends. PMID: 3537615

Versatile Identification of Copy Number Variants with Canvas. PMID: 30039371

From gene mutations to tumours--stem cells in gastrointestinal carcinogenesis. PMID: 16300652

Antibodies against the antibiotics: an overview. PMID: 1958023

Biological effects of asbestos fibers on human cells in vitro--especially on lymphocytes and neutrophils. PMID: 11341562

Phosphosite-Specific Antibodies: A Brief Update on Generation and Applications. PMID: 28185181

Polyclonal origin of mouse skin papillomas. PMID: 2803917

Inflammatory pseudotumor of the liver: case report and review of the literature. PMID: 11283904

Consecutive seeding and transfer of genetic diversity in metastasis. PMID: 31239334

The induction of hapten-specific immunological tolerance and immunity in B lymphocytes. VI. Differential tolerance susceptibility in adult spleen as a function of B-cell maturation level. PMID: 158060

Rational design of peptide vaccines for autoimmune disease: harnessing molecular recognition to fix a broken network. PMID: 12901598

Pleural amyloidosis mimicking mesothelioma: a clinicopathologic study of two cases. PMID: 11510005

Plasma cell granuloma of the pituitary gland. Case report. PMID: 11738674

Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation. PMID: 33928547

VH gene use by HIV type 1-associated lymphoproliferations. PMID: 9007199

Fc receptor-bearing T cells and Ig binding factors as class-specific suppressors of polyclonally activated human B cells. PMID: 2976803

[Malignant histiocytosis (author's transl)]. PMID: 304801

Broad Cytotoxic Targeting of Acute Myeloid Leukemia by Polyclonal Delta One T Cells. PMID: 30894378

Canvas: versatile and scalable detection of copy number variants. PMID: 27153601

Mastocytosis: reactive or neoplastic? PMID: 9378823

Polyclonal Regulatory T Cell Therapy for Control of Inflammation in Kidney Transplants. PMID: 28675676

Polyclonality of multiple sporadic basal cell carcinomas. PMID: 19131949

Polyclonal development of mouse mammary preneoplastic nodules. PMID: 14871812

Clonality assays and megakaryocyte culture techniques in essential thrombocythemia. PMID: 8951770

[Cooperation of B- and T-lymphocytes of the human in vitro and in vivo]. PMID: 6447413

Antibody-based cancer therapies: back to "polyclonals"? PMID: 15258701

Polyclonality in Sclerosing Angiomatoid Nodular Transformation of the Spleen. PMID: 27526296

Chromosomal changes in uroepithelial carcinomas. PMID: 16083510

Alloreactivity and immunosuppressive properties of articular chondrocytes from osteoarthritic cartilage. PMID: 27574269

A practical method for barcoding and size-trimming PCR templates for amplicon sequencing. PMID: 26842354

Role of endothelium in chronic inflammatory synovitis. PMID: 7809908

Polyclonal B lymphocyte activation during Trypanosoma cruzi infection. PMID: 6153088

Azacitidine, Venetoclax, and Magrolimab in Newly Diagnosed and Relapsed Refractory Acute Myeloid Leukemia: Phase Ib/II Study and Correlative Analysis., PMID:40198272

IL13RA2-integrated genetically engineered mouse model allows for CAR T cells targeting pediatric high-grade gliomas., PMID:40176156

Imaging Cell Surface Plectin in PDAC Patients - A First-In-Human Phase 0 Study Report., PMID:40140133

TP53 mutations and TET2 deficiency cooperate to drive leukemogenesis and establish an immunosuppressive environment., PMID:40111422

PARP7 inhibits type I interferon signaling to prevent autoimmunity and lung disease., PMID:39969510

Zanubrutinib, obinutuzumab, and venetoclax for first-line treatment of mantle cell lymphoma with a TP53 mutation., PMID:39437708

An immunoassay based on bioluminescent sensors for rapid detection of African swine fever virus antibodies., PMID:39235247

SARS-CoV-2 Plasma Antibody and Nucleocapsid Antigen Status Predict Outcomes in Outpatients With COVID-19., PMID:39018444

Development of a P30 protein-based indirect ELISA for detecting African swine fever antibodies utilizing the HEK293F expression system., PMID:38936461

Molecular Landscape and Clinical Implication of CCNE1-amplified Esophagogastric Cancer., PMID:38717153

A Modular Trial of Androgen Signaling Inhibitor Combinations Testing a Risk-Adapted Strategy in Patients with Metastatic Castration-Resistant Prostate Cancer., PMID:38683200

Key roles for phosphorylation and the Coiled-coil domain in TRIM56-mediated positive regulation of TLR3-TRIF-dependent innate immunity., PMID:38556084

p53 suppresses MHC class II presentation by intestinal epithelium to protect against radiation-induced gastrointestinal syndrome., PMID:38167344

Widespread BRCA1/2-independent homologous recombination defects are caused by alterations in RNA-binding proteins., PMID:37909041

APR-246 increases tumor antigenicity independent of p53., PMID:37891002

Survey of NF1 inactivation by surrogate immunohistochemistry in ovarian carcinomas., PMID:37820398

Suppression of Tumor Cell Lactate-generating Signaling Pathways Eradicates Murine PTEN/p53-deficient Aggressive-variant Prostate Cancer via Macrophage Phagocytosis., PMID:37721526

Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts., PMID:37402225

Neuro-PASC is characterized by enhanced CD4+ and diminished CD8+ T cell responses to SARS-CoV-2 Nucleocapsid protein., PMID:37313412

Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies., PMID:37026062

Targeting USP2 regulation of VPRBP-mediated degradation of p53 and PD-L1 for cancer therapy., PMID:37024504

Reversal of Lactate and PD-1-mediated Macrophage Immunosuppression Controls Growth of PTEN/p53-deficient Prostate Cancer., PMID:36862086

Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus., PMID:36298725

Neoadjuvant Radiation Therapy and Surgery Improves Metastasis-Free Survival over Surgery Alone in a Primary Mouse Model of Soft Tissue Sarcoma., PMID:36162051

TP53 Sequencing and p53 Immunohistochemistry Predict Outcomes When Bevacizumab Is Added to Frontline Chemotherapy in Endometrial Cancer: An NRG Oncology/Gynecologic Oncology Group Study., PMID:35658479

Cell type-specific biotin labeling in vivo resolves regional neuronal and astrocyte proteomic differences in mouse brain., PMID:35614064

The DNA sensors AIM2 and IFI16 are SLE autoantigens that bind neutrophil extracellular traps., PMID:35608258

TP53-altered chronic lymphocytic leukemia treated with firstline Bruton's tyrosine kinase inhibitor-based therapy: A retrospective analysis., PMID:35567779

Caveolin-1 temporal modulation enhances antibody drug efficacy in heterogeneous gastric cancer., PMID:35534471

Tumor FAK orchestrates immunosuppression in ovarian cancer via the CD155/TIGIT axis., PMID:35467979

PTEN Loss and BRCA1 Promoter Hypermethylation Negatively Predict for Immunogenicity in BRCA-Deficient Ovarian Cancer., PMID:35201851

Endothelial p130cas confers resistance to anti-angiogenesis therapy., PMID:35081345

Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide., PMID:34715492

A Novel Monoclonal Antibody Targeting Cancer-Specific Plectin Has Potent Antitumor Activity in Ovarian Cancer., PMID:34571866

Ibrutinib induces durable remissions in treatment-naïve patients with CLL and 17p deletion and/or TP53 mutations., PMID:34521099

Mild SARS-CoV-2 Illness Is Not Associated with Reinfections and Provides Persistent Spike, Nucleocapsid, and Virus-Neutralizing Antibodies., PMID:34468184

Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy., PMID:34233193

Epigenetic Plasticity Enables CNS-Trafficking of EBV-infected B Lymphocytes., PMID:34106998

An antibody against L1 cell adhesion molecule inhibits cardiotoxicity by regulating persistent DNA damage., PMID:34078883

Ibrutinib, fludarabine, cyclophosphamide, and obinutuzumab (iFCG) regimen for chronic lymphocytic leukemia (CLL) with mutated IGHV and without TP53 aberrations., PMID:34007049

Reverse Phase Protein Array Reveals Correlation of Retinoic Acid Metabolism With Cardiomyopathy in Friedreich's Ataxia., PMID:33991687

Na+/H+ Exchanger Regulatory Factor 1 Mediates the Pathogenesis of Airway Inflammation in a Murine Model of House Dust Mite-Induced Asthma., PMID:33952618

Predicting COVID-19 Severity with a Specific Nucleocapsid Antibody plus Disease Risk Factor Score., PMID:33910993

Genomic, Transcriptomic, and Proteomic Profiling of Metastatic Breast Cancer., PMID:33782032

Targeting a neoantigen derived from a common TP53 mutation., PMID:33649166

Post hoc analyses of GOG 9923: Does BRCA status affect toxicities?: An NRG oncology study., PMID:33610319

Using a Phosphoproteomic Screen to Profile Early Changes During HCMV Infection of Human Monocytes., PMID:33555590

Prevalence of SARS-CoV-2 Antibodies in Children and Adults with Type 1 Diabetes., PMID:33544017

Mutated p53 portends improvement in outcomes when bevacizumab is combined with chemotherapy in advanced/recurrent endometrial cancer: An NRG Oncology study., PMID:33541735

SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus., PMID:33514825

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Phosphoprotein p30/Pret-105 Polyclonal Antibody [PVV02501]
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