Catalog No.
PHJ92201
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human LY96 (Glu17-Asn160).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Ly-96,ESOP-1,MD2,Lymphocyte antigen 96,Protein MD-2,LY96,ESOP1
Purification
Purified by antigen affinity column.
Accession
Q9Y6Y9
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Higher order receptor clustering due to the IgG3 subclass is necessary for TLR4 signaling and tolerance induction by novel human anti-TLR4 antibodies., PMID:40464120
In Silico Analyses Indicate a Lower Potency for Dimerization of TLR4/MD-2 as the Reason for the Lower Pathogenicity of Omicron Compared to Wild-Type Virus and Earlier SARS-CoV-2 Variants., PMID:38791489
Generation and Characterization of a Novel Anti-Rat TLR4/MD2 Antibody with Potent Neutralizing Activity In Vivo., PMID:33290148
Genetic Predictors of Long-term Response to Antitumor Necrosis Factor Agents in Pediatric Inflammatory Bowel Disease., PMID:32773718
THP-1 cells increase TNF-α production upon LPS + soluble human IgG co-stimulation supporting evidence for TLR4 and Fcγ receptors crosstalk., PMID:32702524
Blockade of the TLR4-MD2 complex lowers blood pressure and improves vascular function in a murine model of type 1 diabetes., PMID:32694567
Pharmacogenetics of trough serum anti-TNF levels in paediatric inflammatory bowel disease., PMID:32478906
HIV-1 Tat - TLR4/MD2 interaction drives the expression of IDO-1 in monocytes derived dendritic cells through NF-κB dependent pathway., PMID:32424165
Identification of Potential Core Genes in Immunoglobulin-Resistant Kawasaki Disease Using Bioinformatics Analysis., PMID:32421987
Integrated transcriptomic correlation network analysis identifies COPD molecular determinants., PMID:32099002
A TLR4-derived non-cytotoxic, self-assembling peptide functions as a vaccine adjuvant in mice., PMID:30385503
Computationally Designed Bispecific MD2/CD14 Binding Peptides Show TLR4 Agonist Activity., PMID:30348734
Exploring the biological functional mechanism of the HMGB1/TLR4/MD-2 complex by surface plasmon resonance., PMID:30134799
MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2., PMID:28726347
Associations between functional polymorphisms and response to biological treatment in Danish patients with psoriasis., PMID:28696418
Palmitic acid is a toll-like receptor 4 ligand that induces human dendritic cell secretion of IL-1β., PMID:28463985
Soluble CD83 Inhibits T Cell Activation by Binding to the TLR4/MD-2 Complex on CD14+ Monocytes., PMID:28193829
MYD88 and functionally related genes are associated with multiple infections in a model population of Kenyan village dogs., PMID:27655108
Reversal of New-Onset Type 1 Diabetes With an Agonistic TLR4/MD-2 Monoclonal Antibody., PMID:26130764
Anti-β2GPI/β2GPI stimulates activation of THP-1 cells through TLR4/MD-2/MyD88 and NF-κB signaling pathways., PMID:24157085
MD-2-dependent human Toll-like receptor 4 monoclonal antibodies detect extracellular association of Toll-like receptor 4 with extrinsic soluble MD-2 on the cell surface., PMID:24021278
Inefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A., PMID:23638128
Human TLR4 polymorphism D299G/T399I alters TLR4/MD-2 conformation and response to a weak ligand monophosphoryl lipid A., PMID:22962435
Multiple potential regulatory sites of TLR4 activation induced by LPS as revealed by novel inhibitory human TLR4 mAbs., PMID:22499954
Structure-based rational design of a Toll-like receptor 4 (TLR4) decoy receptor with high binding affinity for a target protein., PMID:22363519
The RP105/MD-1 complex is indispensable for TLR4/MD-2-dependent proliferation and IgM-secreting plasma cell differentiation of marginal zone B cells., PMID:22354914
Effects of endotoxin exposure on childhood asthma risk are modified by a genetic polymorphism in ACAA1., PMID:22151743
Differential effect of inhibiting MD-2 and CD14 on LPS- versus whole E. coli bacteria-induced cytokine responses in human blood., PMID:21948372
Mite allergen Der-p2 triggers human B lymphocyte activation and Toll-like receptor-4 induction., PMID:21909400
Prolonged hepatomegaly in mice that cannot inactivate bacterial endotoxin., PMID:21674560
LPS-induced TLR4 signaling in human colorectal cancer cells increases beta1 integrin-mediated cell adhesion and liver metastasis., PMID:21363926
Toll-like receptor (TLR)-4 mediates anti-β2GPI/β2GPI-induced tissue factor expression in THP-1 cells., PMID:21091668
Regulation of colon cancer cell proliferation and migration by MD-2 activity., PMID:20699280
Toll-like receptor 4 modulation as a strategy to treat sepsis., PMID:20396414
Sera from patients with Crohn's disease break bacterial lipopolysaccharide tolerance of human intestinal epithelial cells via MD-2 activity., PMID:20351135
Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex., PMID:20019359
Lipopolysaccharide-trap-Fc, a multifunctional agent to battle gram-negative bacteria., PMID:19433546
Direct recognition of LPS by human but not murine CD8+ T cells via TLR4 complex., PMID:19405031
Identification of human S100A9 as a novel target for treatment of autoimmune disease via binding to quinoline-3-carboxamides., PMID:19402754
IL-10 enhances MD-2 and CD14 expression in monocytes and the proteins are increased and correlated in HIV-infected patients., PMID:19109192
Inhibition of corneal inflammation by the TLR4 antagonist Eritoran tetrasodium (E5564)., PMID:18936141
Molecular events in human T cells treated with diesel exhaust particles or formaldehyde that underlie their diminished interferon-gamma and interleukin-10 production., PMID:18849615
Paclitaxel binding to human and murine MD-2., PMID:18650420
Mast cell-mediated immune responses through IgE antibody and Toll-like receptor 4 by malarial peroxiredoxin., PMID:18398934
Biological properties of the native and synthetic lipid A of Porphyromonas gingivalis lipopolysaccharide., PMID:18173800
Preparation and characterization of agonistic monoclonal antibodies against Toll-like receptor 4-MD-2 complex., PMID:18158784
TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock., PMID:17947685
Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran., PMID:17803912
Investigation on the agonistic and antagonistic biological activities of synthetic Chlamydia lipid A and its use in in vitro enzymatic assays., PMID:17621554
Human MD-2 discrimination of meningococcal lipid A structures and activation of TLR4., PMID:17545685
For research use only. Not for human or drug use.
