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Anti-Mouse LCAT Polyclonal Antibody

Catalog #:   PMC07201 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P16301
Overview

Catalog No.

PMC07201

Species reactivity

Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse LCAT (Phe25-Glu438).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Phosphatidylcholine-sterol acyltransferase, 2.3.1.43, 1-alkyl-2-acetylglycerophosphocholine esterase, 3.1.1.47, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, Lcat

Purification

Purified by antigen affinity column.

Accession

P16301

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with LCAT antibody (PMC07201) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 50 kDa
    Observed MW: 50 kDa
References

A Rare Case of Autoimmune-Mediated Lecithin:Cholesterol Acyltransferase Insufficiency Manifesting as the Acute Onset of Extremely Hypo-High-Density Lipoprotein-Cholesterolemia and Spontaneous Improvement: A Case Report with a Review of the Literature., PMID:39662947

Two Cases of Acquired High-Density Lipoprotein Deficiency with Immunoglobulin G4-Related Lecithin-Cholesterol Acyltransferase Autoantibody., PMID:36384970

ApoA-I Infusion Therapies Following Acute Coronary Syndrome: Past, Present, and Future., PMID:35524914

Gastric Xanthomatosis Secondary to Lipoprotein X in Primary Biliary Cholangitis., PMID:35403472

Reverse cholesterol transport and hepatic osteodystrophy., PMID:35235770

Novel Insight in Idiopathic Normal Pressure Hydrocephalus (iNPH) Biomarker Discovery in CSF., PMID:34360799

Biological anti-psoriatic therapy profoundly affects high-density lipoprotein function., PMID:33862237

Recombinant human lecithin-cholesterol acyltransferase in patients with atherosclerosis: phase 2a primary results and phase 2b design., PMID:33493256

High-density lipoprotein functionality in systemic lupus erythematosus., PMID:32531506

Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos., PMID:30948018

Immune-mediated acquired lecithin-cholesterol acyltransferase deficiency: A case report and literature review., PMID:29937398

Nephrotic syndrome in infants and children: pathophysiology and management., PMID:28914167

A case of acquired lecithin:cholesterol acyltransferase deficiency with sarcoidosis that remitted spontaneously., PMID:28508975

Antibody Mimetics for the Detection of Small Organic Compounds Using a Quartz Crystal Microbalance., PMID:28192970

Agonistic Human Antibodies Binding to Lecithin-Cholesterol Acyltransferase Modulate High Density Lipoprotein Metabolism., PMID:26644477

The high-resolution crystal structure of human LCAT., PMID:26195816

Higher Cardiovascular Risk in Common Variable Immunodeficiency and X-Linked Agammaglobulinaemia Patients., PMID:26183722

Familial lecithin-cholesterol acyltransferase (LCAT) deficiency; a differential of proteinuria., PMID:25657982

Recombinant human lecithin-cholesterol acyltransferase Fc fusion: analysis of N- and O-linked glycans and identification and elimination of a xylose-based O-linked tetrasaccharide core in the linker region., PMID:24115046

Western blots., PMID:23912997

Nephrotic syndrome caused by immune-mediated acquired LCAT deficiency., PMID:23620397

Severe high-density lipoprotein deficiency associated with autoantibodies against lecithin:cholesterol acyltransferase in non-Hodgkin lymphoma., PMID:22271127

An apoA-I mimetic peptide facilitates off-loading cholesterol from HDL to liver cells through scavenger receptor BI., PMID:19847320

Haptoglobin from psoriatic patients exhibits decreased activity in binding haemoglobin and inhibiting lecithin-cholesterol acyltransferase activity., PMID:18363910

[Detection of qualitative abnormalities of HDL by measurement of prebeta1-HDL concentration]., PMID:17265898

Cholesteryl ester transfer protein promotes the formation of cholesterol-rich remnant like lipoprotein particles in human plasma., PMID:16859664

Assignment of the binding site for haptoglobin on apolipoprotein A-I., PMID:15533931

Poloxamer 407-mediated alterations in the activities of enzymes regulating lipid metabolism in rats., PMID:12935429

LCAT-dependent conversion of prebeta1-HDL into alpha-migrating HDL is severely delayed in hemodialysis patients., PMID:12595510

[Atherosclerosis and sensitive determination of oxidized LDL using monoclonal antibody]., PMID:12400156

A new enzyme-linked immunosorbent assay with two monoclonal antibodies to specific epitopes measures human lecithin-cholesterol acyltransferase., PMID:11861675

Cyclosporine A transfer between high- and low-density lipoproteins: independent from lipid transfer protein I-facilitated transfer of lipoprotein-coated phospholipids because of high affinity of cyclosporine a for the protein component of lipoproteins., PMID:11745783

Probing the 121-136 domain of lecithin:cholesterol acyltransferase using antibodies., PMID:11368007

Identification of domains in apoA-I susceptible to proteolysis by mast cell chymase. Implications for HDL function., PMID:10828090

Classical LCAT deficiency resulting from a novel homozygous dinucleotide deletion in exon 4 of the human lecithin: cholesterol acyltransferase gene causing a frameshift and stop codon at residue 144., PMID:10487497

Metabolism of oxidized phosphatidylcholines formed in oxidized low density lipoprotein by lecithin-cholesterol acyltransferase., PMID:10393333

Possible induction of renal dysfunction in patients with lecithin:cholesterol acyltransferase deficiency by oxidized phosphatidylcholine in glomeruli., PMID:10073988

Effect of apolipoprotein A-I lipidation on the formation and function of pre-beta and alpha-migrating LpA-I particles., PMID:10026251

Branched synthetic peptide constructs mimic cellular binding and efflux of apolipoprotein AI in reconstituted high density lipoproteins., PMID:9862171

Effect of long chain polyunsaturated fatty acids in the sn-2 position of phosphatidylcholine on the interaction with recombinant high density lipoprotein apolipoprotein A-I., PMID:9831630

The hydrophobic face orientation of apolipoprotein A-I amphipathic helix domain 143-164 regulates lecithin:cholesterol acyltransferase activation., PMID:9565601

Deletion of amino acids Glu146-->Arg160 in human apolipoprotein A-I (ApoA-ISeattle) alters lecithin:cholesterol acyltransferase activity and recruitment of cell phospholipid., PMID:9538003

In vitro factors affecting the concentration of gamma-LpE (gamma-LpE) in human plasma., PMID:9555949

A natural apolipoprotein A-I variant, apoA-I (L141R)Pisa, interferes with the formation of alpha-high density lipoproteins (HDL) but not with the formation of pre beta 1-HDL and influences efflux of cholesterol into plasma., PMID:9215551

Cholesteryl ester transfer protein activity enhances plasma cholesteryl ester formation. Studies in CETP transgenic mice and human genetic CETP deficiency., PMID:9194753

Fish-eye disease: structural and in vivo metabolic abnormalities of high-density lipoproteins., PMID:9160810

Alteration in apolipoprotein A-I 22-mer repeat order results in a decrease in lecithin:cholesterol acyltransferase reactivity., PMID:9054424

Characterization of human apolipoprotein A-I expressed in Escherichia coli., PMID:9030191

Localization of apolipoprotein A-I epitopes involved in the activation of lecithin:cholesterol acyltransferase., PMID:9017508

Characterization and potential uses of rabbit polyclonal antibodies against human plasma lecithin-cholesterol acyltransferase., PMID:9181076

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Mouse LCAT Polyclonal Antibody [PMC07201]
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