Catalog No.
PHJ95901
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human RNF168 (Met1-Gln95).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000
Target
E3 ubiquitin-protein ligase RNF168, hRNF168, 2.3.2.27, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168
Concentration
0.73 mg/ml
Purification
Purified by antigen affinity column.
Accession
Q8IYW5
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage., PMID:33710666
Multivariate genome-wide analysis of immunoglobulin G N-glycosylation identifies new loci pleiotropic with immune function., PMID:33710309
DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity., PMID:29656893
DNA Damage-Induced Foci of E2 Ubiquitin-Conjugating Enzyme are Detectable upon Co-transfection with an Interacting E3 Ubiquitin Ligase., PMID:26718580
Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168., PMID:25695757
BCL10 regulates RNF8/RNF168-mediated ubiquitination in the DNA damage response., PMID:24732096
USP3 counteracts RNF168 via deubiquitinating H2A and γH2AX at lysine 13 and 15., PMID:24196443
Three-dimensional imaging reveals the spatial separation of γH2AX-MDC1-53BP1 and RNF8-RNF168-BRCA1-A complexes at ionizing radiation-induced foci., PMID:22633816
53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair., PMID:20081839
The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination., PMID:20080757
A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses., PMID:20075863
For research use only. Not for human or drug use.
