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Anti-Human INSL3 Polyclonal Antibody

Catalog #:   PHE81901 Specific References (26) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P51460
Overview

Catalog No.

PHE81901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human INSL3 (Leu21-Tyr131).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Insulin-like 3, Leydig insulin-like peptide, Ley-I-L, Relaxin-like factor, Insulin-like 3 B chain, Insulin-like 3 A chain, INSL3, RLF, RLNL

Purification

Purified by antigen affinity column.

Accession

P51460

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with INSL3 antibody (PHE81901) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 41 kDa
    Observed MW: 41 kDa
References

Discovery of RXFP2 genetic association in resistant hypertensive men and RXFP2 antagonists for the treatment of resistant hypertension., PMID:38851835

Expression of Insl3 Protein in Adult Danio rerio., PMID:38791457

Endocrine and dog factors associated with semen quality., PMID:38184699

Shortwave radiation-induced reproductive organ damage in male rats by enhanced expression of molecules associated with the calpain/Cdk5 pathway and oxidative stress., PMID:38155529

Isolation of Leydig cells from adult rat testes by magnetic-activated cell sorting protocol based on prolactin receptor expression., PMID:35735181

Association of antimullerian hormone with the size of the appendix testis, the androgen and estrogen receptors and their expression in the appendix testis, in congenital cryptorchidism., PMID:34265880

Role of the kisspeptin/KISS1 receptor system in the testicular development of mice., PMID:33543882

Neurotrophin-3 stimulates stem Leydig cell proliferation during regeneration in rats., PMID:33090725

Role of the Kisspeptin/KISS1 Receptor System in the Testicular Development of Mice., PMID:33044408

Evidence for the role of INSL3 on sperm production in boars by passive immunisation., PMID:29575065

Expression of insulin-like factor 3 hormone-receptor system in the reproductive organs of male goats., PMID:26017634

The insulin-like factor 3 (INSL3)-receptor (RXFP2) network functions as a germ cell survival/anti-apoptotic factor in boar testes., PMID:25562614

The increased number of Leydig cells by di(2-ethylhexyl) phthalate comes from the differentiation of stem cells into Leydig cell lineage in the adult rat testis., PMID:23391632

Fetal testis dysgenesis and compromised Leydig cell function in Tgfbr3 (beta glycan) knockout mice., PMID:19696014

Lysosomal acid hydrolases of the cathepsin family are novel targets of INSL3 in human thyroid carcinoma cells., PMID:19416220

Role of relaxin in human osteoclastogenesis., PMID:19416193

INSLF3-LGR8 ligand-receptor system in testes of mature rats after exposure to ethane dimethanesulphonate (short communication)., PMID:18702224

Structure of the transmembrane signal initiation site of the relaxin-like factor (RLF/INSL3)., PMID:17676766

Insulin 3-like hormone and its role in epididymo-testicular descent., PMID:17626659

Developmental expression and gene regulation of insulin-like 3 receptor RXFP2 in mouse male reproductive organs., PMID:17615407

Expression of insulin-like factor 3 protein in the rat testis during fetal and postnatal development and in relation to cryptorchidism induced by in utero exposure to di (n-Butyl) phthalate., PMID:16037377

Immunohistochemical localization of relaxin-like factor/insulin-like peptide-3 in the bovine corpus luteum., PMID:15956752

Insulin-like factor 3: where are we now?, PMID:15956750

Synthesis, conformation, receptor binding and biological activities of monobiotinylated human insulin-like peptide 3., PMID:15009530

Dynamic changes in the expression of relaxin-like factor (INSL3), cholesterol side-chain cleavage cytochrome p450, and 3beta-hydroxysteroid dehydrogenase in bovine ovarian follicles during growth and atresia., PMID:11906911

Relaxin-like factor (RLF) serum concentrations and gubernaculum RLF receptor display in relation to pre- and neonatal development of rats., PMID:11732985

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human INSL3 Polyclonal Antibody [PHE81901]
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