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Anti-Human DBT/BCOADC-E2 Polyclonal Antibody

Catalog #:   PHC86501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P11182
Overview

Catalog No.

PHC86501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DBT/BCOADC-E2 (Gly258-Lys482).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial, 2.3.1.168, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched chain 2-oxo-acid dehydrogenase complex component E2, BCOADC-E2, Branched-chain alpha-keto acid dehydrogenase complex component E2, BCKAD-E2, BCKADE2, BCKDH-E2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, DBT, BCATE2, BCKDHE2

Purification

Purified by antigen affinity column.

Accession

P11182

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with DBT/BCOADC-E2 antibody (PHC86501) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
References

Development of a lateral flow assay- based rapid diagnostic kit for screening Bovine Trichomonosis in cattle., PMID:40412343

Distinct region-specific neutralization profiles of contemporary HIV-1 clade C against best-in-class broadly neutralizing antibodies., PMID:40377318

Immunogenicity evaluation of respiratory syncytial virus prefusogenic-F based virus-like-particles consisting of G and M proteins in mice., PMID:40334534

Matrix metalloproteinase-9 (MMP-9): A macromolecular mediator in CNS infections: A review., PMID:40328391

Removal of Excipients from Drug Product may Impact Antibody Characterization of Monoclonal Antibodies., PMID:40316853

Assessing HIV-1 subtype C infection dynamics, therapeutic responses and reservoir distribution using a humanized mouse model., PMID:40308596

A novel dominant-negative variant of IRF8 in a mother and son: Clinical, phenotypic and biological characteristics., PMID:40072380

Understanding the physiological role and cross-interaction network of VapBC35 toxin-antitoxin system from Mycobacterium tuberculosis., PMID:40016306

Microbiological Investigations for Chikungunya Virus in Children With Acute Encephalitis Syndrome in a Non-Outbreak Setting in Southern India., PMID:39953947

Nanobody-Oligonucleotide Conjugates (NucleoBodies): The Next Frontier in Oligonucleotide Therapy., PMID:39953265

Effect of kangaroo mother care in low birth weight infants on human milk intake: a randomized controlled trial., PMID:39947481

Role of RGD-binding Integrins in ovarian cancer progression, metastasis and response to therapy., PMID:39916547

Alloanti-D induction in a rare RhD variant (DBT-2) case: Insights from serological and molecular biological testing., PMID:39908659

Concomitant Histological Features of Membranous Nephropathy and Anti-Neutrophil Cytoplasmic Antibody Associated Vasculitis., PMID:39872275

Impact of Various Forced Oxidative Stress Factors in Rapid Degradation of mAb: Trastuzumab as a Case Study., PMID:39849217

Characterization of differentially regulated carboxypeptidase (metallopeptidase M32) protein in Miltefosine resistant Leishmania donovani parasites., PMID:39674486

Inflammation-targeted delivery of Urolithin A mitigates chemical- and immune checkpoint inhibitor-induced colitis., PMID:39533380

The Evolution of Illicit-Drug Detection: From Conventional Approaches to Cutting-Edge Immunosensors-A Comprehensive Review., PMID:39451690

Unveiling the impact of Leptospira TolC efflux protein on host tissue adherence, complement evasion, and diagnostic potential., PMID:39392312

Neutralizing Antibodies against 10 SARS-CoV-2 Variants at Two Years Post-COVISHIELD Vaccination with Special Reference to Omicron Subvariants and Booster Administration., PMID:39340071

7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively., PMID:39284947

The pathogenesis of IgA nephropathy and implications for treatment., PMID:39232245

Integrated viral and immune monitoring in a prospective COVID-19 cohort from India., PMID:39219468

Artificially inserted strong promoter containing multiple G-quadruplexes induces long-range chromatin modification., PMID:39158543

Improved methods for total and chloroplast protein extraction from Cajanus species for two-dimensional gel electrophoresis and mass spectrometry., PMID:39146296

Mucosal delivery of a prefusogenic-F, glycoprotein, and matrix proteins-based virus-like particle respiratory syncytial virus vaccine induces protective immunity as evidenced by challenge studies in mice., PMID:39096774

Injection of antioxidant trace minerals/vitamins into peripartum crossbred cows improves the nutritional and immunological properties of colostrum/milk and the health of their calves under heat stress conditions., PMID:39066797

Exploring How Antibody Format Drives Clearance from the Brain., PMID:39058284

Dengue virus: pathogenesis and potential for small molecule inhibitors., PMID:39051974

L-Cysteine functionalized magnetite nanoparticle adorned Ti3C2-MXene nanohybrid based screen printed immunosensor for oral cancer biomarker detection., PMID:39007760

A(H2N2) and A(H3N2) influenza pandemics elicited durable cross-reactive and protective antibodies against avian N2 neuraminidases., PMID:38961067

Role of Charge Heterogeneity on Physical Stability of Monoclonal Antibody Biotherapeutic Products., PMID:38951451

Coexistence of systemic sclerosis and cryopyrin-associated periodic syndrome., PMID:38940218

In silico studies and development of a protein-based electrochemical sensor for selective and sensitive detection of aflatoxin B1., PMID:38935329

Increased efficacy of influenza virus vaccine candidate through display of recombinant neuraminidase on virus like particles., PMID:38915410

Movement Disorders in Patients with Subacute Sclerosing Panencephalitis: A Systematic Review., PMID:38748762

Disposable graphene-oxide screen-printed electrode integrated with portable device for detection of SARS-CoV-2 in clinical samples., PMID:38697015

Harnessing the Cross-Neutralisation Potential of Existing Antivenoms for Mitigating the Outcomes of Snakebite in Sub-Saharan Africa., PMID:38673799

SARS-CoV-2 infection induces thymic atrophy mediated by IFN-γ in hACE2 transgenic mice., PMID:38655818

An Omicron-specific, self-amplifying mRNA booster vaccine for COVID-19: a phase 2/3 randomized trial., PMID:38637636

Determination, expression and characterization of an UDP-N-acetylglucosamine:α-1,3-D-mannoside β-1,2-N-acetylglucosaminyltransferase I (GnT-I) from the Pacific oyster, Crassostrea gigas., PMID:38557922

B cell responses and antibody-based therapeutic perspectives in human cancers., PMID:38522010

Immunogenicity of SARS-CoV-2 vaccines BBV152 (COVAXIN®) and ChAdOx1 nCoV-19 (COVISHIELD™) in seronegative and seropositive individuals in India: a multicentre, nonrandomised observational study., PMID:38482152

Combination biologic therapy in pediatric inflammatory bowel disease: Safety and efficacy over a minimum 12-month follow-up period., PMID:38477410

B cell repertoire sequencing of HIV-1 pediatric elite-neutralizers identifies multiple broadly neutralizing antibody clonotypes., PMID:38433846

Synthetic development of a broadly neutralizing antibody against snake venom long-chain α-neurotoxins., PMID:38381847

Convolutional Neural Networks Guided Raman Spectroscopy as a Process Analytical Technology (PAT) Tool for Monitoring and Simultaneous Prediction of Monoclonal Antibody Charge Variants., PMID:38366234

Therapeutic potential of tocotrienols as chemosensitizers in cancer therapy., PMID:38353331

Severe disease during both primary and secondary dengue virus infections in pediatric populations., PMID:38321219

Serological and molecular detection of Toxoplasma gondii and Neospora caninum in free-ranging rats from Nagpur, India., PMID:38114841

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Human DBT/BCOADC-E2 Polyclonal Antibody [PHC86501]
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