Catalog No.
PHC34801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SNRNP70 (Asp90-Glu221).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
U1 small nuclear ribonucleoprotein 70 kDa, U1 snRNP 70 kDa, U1-70K, snRNP70, SNRNP70, RNPU1Z, RPU1, SNRP70, U1AP1
Purification
Purified by antigen affinity column.
Accession
P08621
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Integrative analysis of serum proteomics and transcriptomics in hepatitis C., PMID:40082995
Doubtful Clinical Value of Subtyping Anti-U1-RNP Antibodies Regarding the RNP-70 kDa Antigen in Sera of Patients with Systemic Lupus Erythematosus., PMID:37373545
Splicing machinery is impaired in rheumatoid arthritis, associated with disease activity and modulated by anti-TNF therapy., PMID:34625402
Basophils and IgE contribute to mixed connective tissue disease development., PMID:33338538
Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus., PMID:27279139
Mapping epitopes of U1-70K autoantibodies at single-amino acid resolution., PMID:26333287
Expression, localization, and clinical application of the RNA binding domain of U1-70kD in HEp-2 cells., PMID:25887865
Serum antibodies against the 70k polypeptides of the U1 ribonucleoprotein complex are associated with psychiatric syndromes in systemic lupus erythematosus: a retrospective study., PMID:22454191
The molecular basis for the presence of two autoimmune diseases occurring simultaneously--preliminary observations based on computer analysis., PMID:22053914
Anti-U1 RNP antibodies in cerebrospinal fluid are associated with central neuropsychiatric manifestations in systemic lupus erythematosus and mixed connective tissue disease., PMID:20722023
CD4+ T cells target epitopes residing within the RNA-binding domain of the U1-70-kDa small nuclear ribonucleoprotein autoantigen and have restricted TCR diversity in an HLA-DR4-transgenic murine model of mixed connective tissue disease., PMID:18523312
Negative correlation of anti-U1RNP antibody titers and the amount of salivary secretion with age correction., PMID:18310968
[The spliceosome and its interest for lupus therapy]., PMID:17553599
Cloning of B cell lymphoma-associated antigens using modified phage-displayed expression cDNA library and immunized patient sera., PMID:16631194
A murine model of mixed connective tissue disease induced with U1 small nuclear RNP autoantigen., PMID:16453294
Non-snRNP U1A levels decrease during mammalian B-cell differentiation and release the IgM secretory poly(A) site from repression., PMID:16373497
Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease., PMID:15743477
Lack of induction of autoantibody responses following immunization with cytomegalovirus (CMV) glycoprotein B (gB) in healthy CMV-seronegative subjects., PMID:15542191
A major B cell epitope present on the apoptotic but not the intact form of the U1-70-kDa ribonucleoprotein autoantigen., PMID:14688384
T cell recognition and therapeutic effect of a phosphorylated synthetic peptide of the 70K snRNP protein administered in MR/lpr mice., PMID:12548559
Lack of autoantibody production associated with cytomegalovirus infection., PMID:12223109
The genotype of mice influences the autoimmune response to spliceosome proteins induced by cytomegalovirus gB immunization., PMID:12100018
Secondary necrosis is a source of proteolytically modified forms of specific intracellular autoantigens: implications for systemic autoimmunity., PMID:11710720
Immunization with a bacterial ATP-binding cassette transporter fragment suppresses autoimmunity and prolongs survival in MRL/lpr lupus-prone mice., PMID:11199919
Characterization of recombinant human autoantibody fragments directed toward the autoantigenic U1-70K protein., PMID:11069087
Mixed connective tissue disease., PMID:10990174
Characterization of a Trypanosoma brucei SR domain-containing protein bearing homology to cis-spliceosomal U1 70 kDa proteins., PMID:10743615
Autoantibody profiles in canine ANA-positive sera investigated by immunoblot and ELISA., PMID:9613431
Autoepitope-mapping of the U1-70K protein with human-Drosophila chimeric proteins., PMID:9451595
Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing., PMID:9447963
Sequential activation of three distinct ICE-like activities in Fas-ligated Jurkat cells., PMID:8706881
Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes., PMID:7479068
For research use only. Not for human or drug use.
