Catalog No.
PHA74901
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human BUB3 (Met1-Thr328).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Mitotic checkpoint protein BUB3, BUB3
Purification
Purified by antigen affinity column.
Accession
O43684
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
The closed form of Mad2 is bound to Mad1 and Cdc20 at unattached kinetochores., PMID:29895228
Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling., PMID:26986935
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation., PMID:26399325
Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing., PMID:25918225
BuGZ is required for Bub3 stability, Bub1 kinetochore function, and chromosome alignment., PMID:24462187
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction., PMID:22331848
Effects of artesunate on cytokinesis and G₂/M cell cycle progression of tumour cells and budding yeast., PMID:21156967
Benzo[a]pyrene treatment leads to changes in nuclear protein expression and alternative splicing., PMID:20097212
The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly., PMID:16807356
The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis., PMID:15615783
The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1., PMID:15556864
Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells., PMID:15075237
Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts., PMID:12538762
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity., PMID:12514103
Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores., PMID:11451998
Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity., PMID:11402067
Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins., PMID:10607828
Retention of the BUB3 checkpoint protein on lagging chromosomes., PMID:10411903
Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod., PMID:9914369
Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast., PMID:7593191
Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes., PMID:7622604
For research use only. Not for human or drug use.
