Catalog No.
PXX19401
Species reactivity
Escherichia coli (strain K12)
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant E. coli phoA (Arg22-Lys471).
Tested applications
ELISA: 1:4000-1:8000, WB: 1:1000-1:4000
Target
Alkaline phosphatase, APase, 3.1.3.1, phoA, b0383, JW0374
Purification
Purified by antigen affinity column.
Accession
P00634
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Boosting toxic protein biosynthesis: transient in vivo inactivation of engineered bacterial alkaline phosphatase., PMID:32811518
Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins., PMID:32599779
A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli., PMID:33654866
A general platform for efficient extracellular expression and purification of Fab from Escherichia coli., PMID:30887174
The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis., PMID:30732606
Efficient production of human zona pellucida-3 in a prokaryotic expression system., PMID:30341457
Affinity-Based Purification of Polyisocyanopeptide Bioconjugates., PMID:28846388
Secretory expression of a novel human spermatozoa antigen in E. coli and its application to a protein chip., PMID:28600648
Expression, purification of metallothionein genes from freshwater crab (Sinopotamon yangtsekiense) and development of an anti-metallothionein ELISA., PMID:28350826
[Preparation and characterization of monoclonal antibodies against metallothionein of freshwater crab (Sinopotamon henanense)]., PMID:25108438
Encoded protein from ycbR gene of enterohemorrhagic Escherichia coli O157:H7 associated with adherence to HEp-2 cells., PMID:24680289
Chlamydia trachomatis secretion of an immunodominant hypothetical protein (CT795) into host cell cytoplasm., PMID:21441519
Production and characterization of a single-chain Fv antibody-alkaline phosphatase fusion protein specific for clenbuterol., PMID:20087689
Nitazoxanide inhibits biofilm production and hemagglutination by enteroaggregative Escherichia coli strains by blocking assembly of AafA fimbriae., PMID:20086145
Methods for rapid identification of a functional single-chain variable fragment using alkaline phosphatase fusion., PMID:19944014
Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism., PMID:19234130
Characterization of Vibrio cholerae outer membrane vesicles as a candidate vaccine for cholera., PMID:19001078
SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli., PMID:18504019
Epitope mapping of conformational monoclonal antibodies specific to NhaA Na+/H+ antiporter: structural and functional implications., PMID:18452948
New display vector reduces biological bias for expression of antibodies in E. coli., PMID:17267141
Lipoprotein PssN of Rhizobium leguminosarum bv. trifolii: subcellular localization and possible involvement in exopolysaccharide export., PMID:16980497
Identification of a two-partner secretion locus of enterotoxigenic Escherichia coli., PMID:16552055
Expression of recombinant alkaline phosphatase conjugates in Escherichia coli., PMID:15269418
Toxicity-based selection of Escherichia coli mutants for functional recombinant protein production: application to an antibody fragment., PMID:15252207
Bacterial expression of a human monoclonal antibody-alkaline phosphatase conjugate specific for Entamoeba histolytica., PMID:14715571
Epitope analysis of the FanC subunit protein of the K99 (F5) fimbriae of enterotoxigenic Escherichia coli using a recombinant fusion technique., PMID:12208603
Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of selected VH to histochemistry., PMID:11861066
The elastin-binding protein of Staphylococcus aureus (EbpS) is expressed at the cell surface as an integral membrane protein and not as a cell wall-associated protein., PMID:11684686
Regulation of the cytotoxic enterotoxin gene in Aeromonas hydrophila: characterization of an iron uptake regulator., PMID:11553581
Recombinant anti-stefin A Fab fragment: sequence analysis of the variable region and expression in Escherichia coli., PMID:11209759
Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library., PMID:11173521
Open sandwich enzyme-linked immunosorbent assay for the quantitation of small haptens., PMID:11067746
Phage display of cDNA repertoires: the pVI display system and its applications for the selection of immunogenic ligands., PMID:10648926
Open sandwich ELISA with V(H)-/V(L)-alkaline phosphatase fusion proteins., PMID:10357217
pSKAP/S: An expression vector for the production of single-chain Fv alkaline phosphatase fusion proteins., PMID:10336861
Rabbit monoclonal Fab derived from a phage display library., PMID:9692852
Construction, bacterial expression, and characterization of hapten-specific single-chain Fv and alkaline phosphatase fusion protein., PMID:9378709
Construction and characterization of versatile cloning vectors for efficient delivery of native foreign proteins to the periplasm of Escherichia coli., PMID:9435018
Topological analysis of NhaA, a Na+/H+ antiporter from Escherichia coli., PMID:8943289
Analysis of the C-terminal secretion signal of the Rhizobium leguminosarum nodulation protein NodO; a potential system for the secretion of heterologous proteins during nodule invasion., PMID:8870266
Characterization of Escherichia coli expressing an Lpp'OmpA(46-159)-PhoA fusion protein localized in the outer membrane., PMID:8920186
A TnphoA insertion within the Bradyrhizobium japonicum sipS gene, homologous to prokaryotic signal peptidases, results in extensive changes in the expression of PBM-specific nodulins of infected soybean (Glycine max) cells., PMID:8825087
Evidence for in vivo but not in vitro expression of a Borrelia burgdorferi outer surface protein F (OspF) homologue., PMID:8748034
Identification of mycoplasma membrane proteins by systematic Tn phoA mutagenesis of a recombinant library., PMID:8709847
Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection., PMID:7745247
Macrophage-inducible expression of a model antigen in Salmonella typhimurium enhances immunogenicity., PMID:7708746
Genetic approaches to study Legionella pneumophila pathogenicity., PMID:8049098
Specific immunoglobulin cDNA clones produced from hybridoma cell lines and murine spleen fragment cultures by 3SR amplification., PMID:7920236
Processing and surface presentation of the Mycoplasma hyorhinis variant lipoprotein VlpC., PMID:7512554
Application of an alkaline phosphatase fusion protein system suitable for efficient screening and production of Fab-enzyme conjugates in Escherichia coli., PMID:7764723
For research use only. Not for human or drug use.
