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Anti-Snake SVMP/Alfimeprase Polyclonal Antibody

Catalog #:   PWN53601 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, WB
Accession: P28891
Overview

Catalog No.

PWN53601

Species reactivity

Agkistrodon contortrix contortrix (Southern copperhead)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Snake SVMP/Alfimeprase (Phe4-Pro203).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Snake venom metalloproteinase fibrolase, SVMP, 3.4.24.72, Fibrinolytic metalloproteinase, Fibrinolytic proteinase, Alfimeprase

Purification

Purified by antigen affinity column.

Accession

P28891

Applications

ELISA, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SVMP/Alfimeprase antibody (PWN53601) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
References

Comparative in vitro immunoreactivity and protein analysis of Trimeresurus albolabris and Tropidolaemus wagleri venoms., PMID:40221593

First Look at the Venoms of Two Sinomicrurus Snakes: Differences in Yield, Proteomic Profiles, and Immunorecognition by Commercial Antivenoms., PMID:39852972

Deciphering toxico-proteomics of Asiatic medically significant venomous snake species: A systematic review and interactive data dashboard., PMID:39393539

Identification of antigenic proteins from the venom of Malaysian snakes using immunoprecipitation assay and tandem mass spectrometry (LC-MS/MS)., PMID:39286227

Importance of the Cysteine-Rich Domain of Snake Venom Prothrombin Activators: Insights Gained from Synthetic Neutralizing Antibodies., PMID:39195771

A comparison of the venom proteomes and potential therapeutics of 3 African naja subgenera., PMID:38838860

Doxycycline-Mediated Inhibition of Snake Venom Phospholipase and Metalloproteinase., PMID:38748405

Immunochemical Recognition of Bothrops rhombeatus Venom by Two Polyvalent Antivenoms., PMID:38535818

Comparative analysis of Deinagkistrodon acutus venom from Taiwan and China utilizing chromatographic, electrophoretic, and bioinformatic approaches, along with ELISA employing a monospecific antivenom., PMID:38423218

Evaluation of venom diversity and antivenom quality from the venom of long-term captive vs recently wild captured Pseudocerastes persicus snake: An In vitro and In vivo study., PMID:38417708

Kaempferol from Moringa oleifera demonstrated potent antivenom activities via inhibition of metalloproteinase and attenuation of Bitis arietans venom-induced toxicities., PMID:37558138

Bothrops atrox venom: Biochemical properties and cellular phenotypes of three highly toxic classes of toxins., PMID:37442518

Inhibitory Effects of Varespladib, CP471474, and Their Potential Synergistic Activity on Bothrops asper and Crotalus durissus cumanensis Venoms., PMID:36500682

A current perspective on snake venom composition and constituent protein families., PMID:36437303

A Combined Bioassay and Nanofractionation Approach to Investigate the Anticoagulant Toxins of Mamba and Cobra Venoms and Their Inhibition by Varespladib., PMID:36355986

Venom Variation of Neonate and Adult Chinese Cobras in Captivity Concerning Their Foraging Strategies., PMID:36136536

Two snakebite antivenoms have potential to reduce Eswatini's dependency upon a single, increasingly unavailable product: Results of preclinical efficacy testing., PMID:36108067

Biochemistry and toxicology of proteins and peptides purified from the venom of Vipera berus berus., PMID:35769869

Biological and proteomic characterization of the venom from Peruvian Andes rattlesnake Crotalus durissus., PMID:34968566

Development of Antibody Detection ELISA Based on Immunoreactive Toxins and Toxin-Derived Peptides to Evaluate the Neutralization Potency of Equine Plasma against Naja atra in Taiwan., PMID:34822602

Involvement of von Willebrand factor and botrocetin in the thrombocytopenia induced by Bothrops jararaca snake venom., PMID:34478462

Immunorecognition capacity of Indian polyvalent antivenom against venom toxins from two populations of Echis carinatus., PMID:34474069

The Search for Natural and Synthetic Inhibitors That Would Complement Antivenoms as Therapeutics for Snakebite Envenoming., PMID:34209691

Basement membrane degradation and inflammation play a role in the pulmonary hemorrhage induced by a P-III snake venom metalloproteinase., PMID:33872676

Examination of the Efficacy and Cross-Reactivity of a Novel Polyclonal Antibody Targeting the Disintegrin Domain in SVMPs to Neutralize Snake Venom., PMID:33807363

Snake venom proteome of Protobothrops mucrosquamatus in Taiwan: Delaying venom-induced lethality in a rodent model by inhibition of phospholipase A2 activity with varespladib., PMID:33359941

Gamma irradiated protease from Echis pyramidum venom: A promising immunogen to improve viper bites treatment., PMID:33065201

Differential coagulotoxicity of metalloprotease isoforms from Bothrops neuwiedi snake venom and consequent variations in antivenom efficacy., PMID:32841740

Venom variation in Bothrops asper lineages from North-Western South America., PMID:32829066

The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox)., PMID:32511239

Proteomic Analysis of Sri Lanka Echis carinatus Venom: Immunological Cross-Reactivity and Enzyme Neutralization Potency of Indian Polyantivenom., PMID:32501701

Proteomic and toxinological characterization of Peruvian pitviper Bothrops brazili ("jergón shushupe"), venom., PMID:32479836

Generation and In-planta expression of a recombinant single chain antibody with broad neutralization activity on Bothrops pauloensis snake venom., PMID:32035152

Amplification of Snake Venom Toxicity by Endogenous Signaling Pathways., PMID:31979014

Comprehensive Study of the Proteome and Transcriptome of the Venom of the Most Venomous European Viper: Discovery of a New Subclass of Ancestral Snake Venom Metalloproteinase Precursor-Derived Proteins., PMID:31017792

Functional variability of Bothrops atrox venoms from three distinct areas across the Brazilian Amazon and consequences for human envenomings., PMID:30991062

Venom Composition in a Phenotypically Variable Pit Viper ( Trimeresurus insularis) across the Lesser Sunda Archipelago., PMID:30958009

Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey., PMID:30763806

Venomics of Trimeresurus (Popeia) nebularis, the Cameron Highlands Pit Viper from Malaysia: Insights into Venom Proteome, Toxicity and Neutralization of Antivenom., PMID:30736335

Vipera berus berus Venom from Russia: Venomics, Bioactivities and Preclinical Assessment of Microgen Antivenom., PMID:30717298

Human Monoclonal scFvs that Neutralize Fribrinogenolytic Activity of Kaouthiagin, a Zinc-Metalloproteinase in Cobra (Naja kaouthia) Venom., PMID:30513883

Identification of a linear B-cell epitope in the catalytic domain of bothropasin, a metalloproteinase from Bothrops jararaca snake venom., PMID:30399490

Venom proteome of Bungarus sindanus (Sind krait) from Pakistan and in vivo cross-neutralization of toxicity using an Indian polyvalent antivenom., PMID:30385415

Proteomic profile, biological activities and antigenic analysis of the venom from Bothriopsis bilineata smaragdina ("loro machaco"), a pitviper snake from Peru., PMID:30048773

Proteomics, functional characterization and antivenom neutralization of the venom of Pakistani Russell's viper (Daboia russelii) from the wild., PMID:29729992

Transcriptomics-guided bottom-up and top-down venomics of neonate and adult specimens of the arboreal rear-fanged Brown Treesnake, Boiga irregularis, from Guam., PMID:29292096

Haemorrhagic snake venom metalloproteases and human ADAMs cleave LRP5/6, which disrupts cell-cell adhesions in vitro and induces haemorrhage in vivo., PMID:28425175

Combined venomics, antivenomics and venom gland transcriptome analysis of the monocoled cobra (Naja kaouthia) from China., PMID:28263888

Proteomic, toxicological and immunogenic characterization of Mexican west-coast rattlesnake (Crotalus basiliscus) venom and its immunological relatedness with the venom of Central American rattlesnake (Crotalus simus)., PMID:28238904

Snake Venom Metalloproteinases and Their Peptide Inhibitors from Myanmar Russell's Viper Venom., PMID:28042812

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Snake SVMP/Alfimeprase Polyclonal Antibody [PWN53601]
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