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Anti-Human MAD2L1/MAD2 Antibody (SAA2161)

Catalog #:   RHG46201 Specific References (47) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: IF
Accession: Q13257
Overview

Catalog No.

RHG46201

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13257

Applications

IF

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

SAA2161

Data Image
References

Single-cell analysis of neoplastic plasma cells identifies myeloma pathobiology mediators and potential targets., PMID:39855192

Differential anti-viral response to respiratory syncytial virus A in preterm and term infants., PMID:38447274

Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes., PMID:30464155

MAD2 Combined with Mitotic Spindle Apparatus (MSA) and Anticentromere Antibody (ACA) for Diagnosis of Small Cell Lung Cancer (SCLC)., PMID:30346937

The closed form of Mad2 is bound to Mad1 and Cdc20 at unattached kinetochores., PMID:29895228

BRCA1 and MAD2 Are Coexpressed and Are Prognostic Indicators in Tubo-ovarian High-Grade Serous Carcinoma., PMID:29465507

Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling., PMID:26986935

The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation., PMID:26912231

Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint., PMID:26031201

Deregulation of HMGA1 expression induces chromosome instability through regulation of spindle assembly checkpoint genes., PMID:26009897

Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors., PMID:24133140

MYCN-mediated overexpression of mitotic spindle regulatory genes and loss of p53-p21 function jointly support the survival of tetraploid neuroblastoma cells., PMID:23186832

EB1 acetylation by P300/CBP-associated factor (PCAF) ensures accurate kinetochore-microtubule interactions in mitosis., PMID:23001180

Tripeptidyl peptidase II in human oral squamous cell carcinoma., PMID:22986808

Expression of the mitotic-arrest deficiency 2 is associated with chemotherapy resistance in ovarian serous adenocarcinoma., PMID:22797604

Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes., PMID:22513372

Timing of anaphase-promoting complex activation in mouse oocytes is predicted by microtubule-kinetochore attachment but not by bivalent alignment or tension., PMID:22513370

Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint., PMID:21772247

Mps1 at kinetochores is essential for female mouse meiosis I., PMID:21558374

Toward a comprehensive characterization of the phosphotyrosine proteome., PMID:21447384

Effects of artesunate on cytokinesis and G₂/M cell cycle progression of tumour cells and budding yeast., PMID:21156967

MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes., PMID:20406800

Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpoint., PMID:19558549

Spindle assembly checkpoint-related failure perturbs early embryonic divisions and reduces reproductive performance of LT/Sv mice., PMID:19279200

Metaphase I arrest in LT/Sv mouse oocytes involves the spindle assembly checkpoint., PMID:18753610

Perturbation of the chromosomal binding of RCC1, Mad2 and survivin causes spindle assembly defects and mitotic catastrophe., PMID:18712773

BRCA1 is required for meiotic spindle assembly and spindle assembly checkpoint activation in mouse oocytes., PMID:18596218

Aberrant MAD2 expression in soft-tissue sarcoma., PMID:18477210

PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores., PMID:17998396

Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles., PMID:17504913

Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry., PMID:17242465

2-methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes., PMID:17229934

A new Mad2-interacting domain of Cdc20 is critical for the function of Mad2-Cdc20 complex in the spindle assembly checkpoint., PMID:16497171

Mad2 is required for inhibiting securin and cyclin B degradation following spindle depolymerisation in meiosis I mouse oocytes., PMID:16322543

Frequent mutations of human Mad2, but not Bub1, in gastric cancers cause defective mitotic spindle checkpoint., PMID:16112690

Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores., PMID:15964272

Dynamics of centromere and kinetochore proteins; implications for checkpoint signaling and silencing., PMID:15182667

Intra-oocyte localization of MAD2 and its relationship with kinetochores, microtubules, and chromosomes in rat oocytes during meiosis., PMID:15115722

Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest., PMID:14691134

Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores., PMID:14654001

Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension., PMID:12388768

Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint., PMID:11707408

Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores., PMID:11451998

Mitotic checkpoint proteins HsMAD1 and HsMAD2 are associated with nuclear pore complexes in interphase., PMID:11181178

Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events., PMID:9628895

Cell cycle checkpoints: arresting progress in mitosis., PMID:9103187

Identification of a human mitotic checkpoint gene: hsMAD2., PMID:8824189

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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Anti-Human MAD2L1/MAD2 Antibody (SAA2161) [RHG46201]
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