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Anti-TRAF4 Polyclonal Antibody

Catalog #:   PHJ50001 Specific References (11) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9BUZ4
Overview

Catalog No.

PHJ50001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRAF4 (Gln193-Asp444).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

CART1,MLN62,RING finger protein 83,TRAF4,MLN 62,Cysteine-rich domain associated with RING and Traf domains protein 1,RNF83,Metastatic lymph node gene 62 protein,TNF receptor-associated factor 4

Purification

Purified by antigen affinity column.

Accession

Q9BUZ4

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TRAF4 antibody (PHJ50001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
  • Immunocytochemistry/ Immunofluorescence
    TRAF4 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with TRAF4 (PHJ50001) at 6.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

TRAF4 hyperactivates HER2 signaling and contributes to Trastuzumab resistance in HER2-positive breast cancer., PMID:35864174

In vivo evidence that TRAF4 is required for central nervous system myelin homeostasis., PMID:22363515

Delineation of apoptotic genes for synergistic apoptosis of lexatumumab and anthracyclines in human renal cell carcinoma cells by polymerase chain reaction array., PMID:22205156

Role for Traf4 in polarizing adherens junctions as a prerequisite for efficient cell shape changes., PMID:21986496

Involvement of IRAKs and TRAFs in anti-β₂GPI/β₂GPI-induced tissue factor expression in THP-1 cells., PMID:21901230

Characterization of immune functions in TRAF4-deficient mice., PMID:18284467

Tumor necrosis factor receptor-associated factor (TRAF) 4 is a new binding partner for the p70S6 serine/threonine kinase., PMID:12801526

Expression of the tumor necrosis factor receptor-associated factors (TRAFs) 1 and 2 is a characteristic feature of Hodgkin and Reed-Sternberg cells., PMID:11144929

CD40 ligand-induced apoptosis is Fas-independent in human multiple myeloma cells., PMID:10784400

p38 MAPK is required for CD40-induced gene expression and proliferation in B lymphocytes., PMID:9759836

TRAF-4 expression in epithelial progenitor cells. Analysis in normal adult, fetal, and tumor tissues., PMID:9626059

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-TRAF4 Polyclonal Antibody [PHJ50001]
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