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Anti-YWHAZ Polyclonal Antibody

Catalog #:   PHF57101 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P63104
Overview

Catalog No.

PHF57101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human YWHAZ (Met1-Asn245).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

Purification

Purified by antigen affinity column.

Accession

P63104

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with YWHAZ antibody (PHF57101) at 1 µg/ml.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: NIH3T3 cell lysate
    Lane 4: Raji cell lysate
    Lane 5: A549 cell lysate
    Lane 6: K562 cell lysate
    Lane 7: Hela cell lysate
    Lane 8: PC-12 cell lysate
    Lane 9: Mouse brain lysate
    Lane 10: Mouse kidney lysate
    Lane 11: Rat brain lysate
    Lane 12: Rat kidney lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with YWHAZ antibody (PHF57101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
References

Analysing transcriptomic signatures and identifying potential genes for the protective effect of inactivated COVID-19 vaccines., PMID:37096063

Cannabinoid Receptor 1 associates to different molecular complexes during GABAergic neuron maturation., PMID:33942314

The inhibition of bone morphogenetic protein 1 attenuates endometriosis lesions in vivo and in vitro., PMID:32524385

Identification of Reference Genes in Chicken Intraepithelial Lymphocyte Natural Killer Cells Infected with Very-virulent Infectious Bursal Disease Virus., PMID:32444639

Influence of elevated protein and tannin-rich peanut skin supplementation on growth performance, blood metabolites, carcass traits and immune-related gene expression of grazing meat goats., PMID:31724236

Autoantibody against 14-3-3 zeta: a serological marker in detection of gastric cancer., PMID:30887154

Factors of the bone marrow microniche that support human plasma cell survival and immunoglobulin secretion., PMID:30209264

Identification of 14‑3‑3ζ as a potential biomarker in gastric cancer by proteomics‑based analysis., PMID:28944820

Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma., PMID:28596685

Phosphoproteomic Analysis Using the WW and FHA Domains as Biological Filters., PMID:26119529

A cancer-related protein 14-3-3ζ is a potential tumor-associated antigen in immunodiagnosis of hepatocellular carcinoma., PMID:24390614

High-risk human papillomavirus E6 oncoproteins interact with 14-3-3ζ in a PDZ binding motif-dependent manner., PMID:23175360

Short hairpin RNA targeting beta-catenin suppresses cell proliferation and induces apoptosis in human gastric carcinoma cells., PMID:19958059

Protein kinase C-zeta-induced phosphorylation of Ser318 in insulin receptor substrate-1 (IRS-1) attenuates the interaction with the insulin receptor and the tyrosine phosphorylation of IRS-1., PMID:15069075

Protein import into mammalian mitochondria. Characterization of the intermediates along the import pathway of the precursor into the matrix., PMID:8703020

cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol., PMID:7822263

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-YWHAZ Polyclonal Antibody [PHF57101]
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