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Anti-LXN Polyclonal Antibody

Catalog #:   PHJ49401 Specific References (14) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9BS40
Overview

Catalog No.

PHJ49401

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LXN (Met1-Glu222).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Latexin,Tissue carboxypeptidase inhibitor,LXN,Endogenous carboxypeptidase inhibitor,TCI,ECI,Protein MUM

Purification

Purified by antigen affinity column.

Accession

Q9BS40

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with LXN antibody (PHJ49401) at 0.6μg/ml.

    Lane 1: A549 cell lysate
    Lane 2: U87MG cell lysate
    Lane 3: A2780 cell lysate
    Lane 4: PANC1 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 30 kDa
  • Immunocytochemistry/ Immunofluorescence
    LXN in A2780 Cell Line.
    The A2780 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with LXN (PHJ49401) at 12 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with LXN antibody (PHJ49401) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
References

Preoperative camrelizumab combined with chemotherapy for borderline resectable ESCC: A single-arm, prospective, phase 2 study., PMID:39121854

Covalent organic framework-based immunosensor to detect plasma Latexin reveals novel biomarker for coronary artery diseases., PMID:37996165

Morphologic, phenotypic, and transcriptomic characterization of classically and alternatively activated canine blood-derived macrophages in vitro., PMID:28817687

Latexin expression is downregulated in human gastric carcinomas and exhibits tumor suppressor potential., PMID:21466706

Early onset of rejection in concordant hamster xeno hearts display signs of necrosis, but not apoptosis, correlating to the phosphocreatine concentration., PMID:14551030

Localization of pectins in the pollen tube wall of Ornithogalum virens L. Does the pattern of pectin distribution depend on the growth rate of the pollen tube?, PMID:10787057

Latexin, a carboxypeptidase A inhibitor, is expressed in rat peritoneal mast cells and is associated with granular structures distinct from secretory granules and lysosomes., PMID:10698712

Corticocortical associative neurons expressing latexin: specific cortical connectivity formed in vivo and in vitro., PMID:10498275

Cerebral cortical specification by early potential restriction of progenitor cells and later phenotype control of postmitotic neurons., PMID:9895311

Mapping epitopes of neutralizing monoclonal antibodies using phage random peptide libraries., PMID:9281855

Purification, cDNA cloning, functional expression, and characterization of a 26-kDa endogenous mammalian carboxypeptidase inhibitor., PMID:8618874

Latexin: a molecular marker for regional specification in the neocortex., PMID:7808696

Intracortical regionality represented by specific transcription for a novel protein, latexin., PMID:7524963

Cogeneration of neurons with a unique molecular phenotype in layers V and VI of widespread lateral neocortical areas in the rat., PMID:8158255

Datasheet

Document Download

Anti-LXN Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-LXN Polyclonal Antibody [PHJ49401]
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