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Anti-SEMA3C Polyclonal Antibody

Catalog #:   PHJ48201 Specific References (13) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q99985
Overview

Catalog No.

PHJ48201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SEMA3C (Arg28-Asp150).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

SEMA3C,Semaphorin-E,Semaphorin-3C,SEMAE,Sema E

Purification

Purified by antigen affinity column.

Accession

Q99985

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with SEMA3C antibody (PHJ48201) at 1μg/ml.

    Lane 1: Raw264.7 cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: PC12 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Mouse lung lysate
    Lane 6: Mouse small intestine lysate
    Lane 7: Mouse heart lysate
    Lane 8: Rat lung lysate
    Lane 9: Rat small intestine lysate
    Lane 10: Rat heart lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 85 kDa
    Observed MW: 75 kDa
  • Immunohistochemical
    Immunohistochemical analysis of Mouse intestine stained for SEMA3C with PHJ48201.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SEMA3C antibody (PHJ48201) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
References

[The expression of semaphorins in synovial tissue of knee joint in patients with rheumatoid arthritis is increased and positively correlated with clinical inflammatory markers]., PMID:35356884

Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and Semaphorins., PMID:31300520

Semaphorin 3C as a Therapeutic Target in Prostate and Other Cancers., PMID:30759745

Opposing Effects of Neuropilin-1 and -2 on Sensory Nerve Regeneration in Wounded Corneas: Role of Sema3C in Ameliorating Diabetic Neurotrophic Keratopathy., PMID:30679185

Down-regulation of semaphorin 3F in rat retinal ganglion cells in response to optic nerve crush., PMID:27309587

Semaphorin 3C is a novel adipokine linked to extracellular matrix composition., PMID:23666167

Integration of opposing semaphorin guidance cues in cortical axons., PMID:22368082

Lack of action of exogenously administered T3 on the fetal rat brain despite expression of the monocarboxylate transporter 8., PMID:21303950

Axon responses of embryonic stem cell-derived dopaminergic neurons to semaphorins 3A and 3C., PMID:19859963

Semaphorins 3A, 3C, and 3F in mesencephalic dopaminergic axon pathfinding., PMID:18041777

Meningeal cell-derived semaphorin 3A inhibits neurite outgrowth., PMID:14697657

Differential responsiveness to the chemorepellent Semaphorin 3A distinguishes Ipsi- and contralaterally projecting axons in the chick midbrain., PMID:11543622

Neuroendocrine cells along the digestive tract express neuropilin-2., PMID:11394892

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-SEMA3C Polyclonal Antibody [PHJ48201]
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