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Anti-Human RACK1 Polyclonal Antibody

Catalog #:   PHF60301 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P63244
Overview

Catalog No.

PHF60301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RACK1 (Met1-Arg317).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RACK1, Human lung cancer oncogene 7 protein, Guanine nucleotide-binding protein subunit beta-like protein 12.3, GNB2L1, Cell proliferation-inducing gene 21 protein, Guanine nucleotide-binding protein subunit beta-2-like 1, Receptor of activated protein C kinase 1, Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed, Receptor for activated C kinase, Small ribosomal subunit protein RACK1, HLC-7

Purification

Purified by antigen affinity column.

Accession

P63244

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RACK1 antibody (PHF60301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 79 kDa
    Observed MW: 79 kDa
References

RACK1 promotes the development and function of alveolar macrophages through directly binding to and stabilizing PPARγ., PMID:40512793

An Antagonist Antibody That Inhibits Cancer Cell Growth In Vitro through RACK1., PMID:39458945

Rack1 regulates B-cell development and function by binding to and stabilizing the transcription factor Pax5., PMID:39256480

RACK1 enhances STAT3 stability and promotes T follicular helper cell development and function during blood-stage Plasmodium infection in mice., PMID:39024388

Toxoplasma gondii surface antigen 1 (SAG1) interacts in vitro with host cell receptor for activated C kinase 1 (RACK1)., PMID:38157925

Targeting RACK1 to alleviate TDP-43 and FUS proteinopathy-mediated suppression of protein translation and neurodegeneration., PMID:38111057

Longitudinal transcriptional analysis of peripheral blood leukocytes in COVID-19 convalescent donors., PMID:36510222

Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells., PMID:35265209

Invariable Ribosome Stoichiometry During Murine Erythroid Differentiation: Implications for Understanding Ribosomopathies., PMID:35187080

Rack1 is essential for corticogenesis by preventing p21-dependent senescence in neural stem cells., PMID:34469723

Integrin β3, a RACK1 interacting protein, is critical for porcine reproductive and respiratory syndrome virus infection and NF-κB activation in Marc-145 cells., PMID:32247758

Rack1 Controls Parallel Fiber-Purkinje Cell Synaptogenesis and Synaptic Transmission., PMID:31920545

Voltage-Dependent Anion Channel Protein 2 (VDAC2) and Receptor of Activated Protein C Kinase 1 (RACK1) Act as Functional Receptors for Lymphocystis Disease Virus Infection., PMID:30918079

Opposite regulation of Wnt/β-catenin and Shh signaling pathways by Rack1 controls mammalian cerebellar development., PMID:30765517

RACK1 deficiency synergizes with all-trans retinoic acid to induce apoptosis in human acute promyelocytic leukemia cells., PMID:30019299

A label-free electrochemical biosensor for direct detection of RACK 1 by using disposable, low-cost and reproducible ITO based electrode., PMID:29776548

Interaction of the Mineralocorticoid Receptor With RACK1 and Its Role in Aldosterone Signaling., PMID:28472300

Integrin beta and receptor for activated protein kinase C are involved in the cell entry of Bombyx mori cypovirus., PMID:28175946

Label-free, ITO-based immunosensor for the detection of a cancer biomarker: Receptor for Activated C Kinase 1., PMID:27454557

Inhibition of RACK1 ameliorates choroidal neovascularization formation in vitro and in vivo., PMID:27112838

Ion channel TRPM8 promotes hypoxic growth of prostate cancer cells via an O2 -independent and RACK1-mediated mechanism of HIF-1α stabilization., PMID:25065497

A multiparametric serum marker panel as a complementary test to mammography for the diagnosis of node-negative early-stage breast cancer and DCIS in young women., PMID:24957886

Essential function for PDLIM2 in cell polarization in three-dimensional cultures by feedback regulation of the β1-integrin-RhoA signaling axis., PMID:24863845

Luteolin sensitizes the antiproliferative effect of interferon α/β by activation of Janus kinase/signal transducer and activator of transcription pathway signaling through protein kinase A-mediated inhibition of protein tyrosine phosphatase SHP-2 in cancer cells., PMID:24333668

Cancer-derived immunoglobulin G promotes tumor cell growth and proliferation through inducing production of reactive oxygen species., PMID:24309932

Early activation of nSMase2/ceramide pathway in astrocytes is involved in ischemia-associated neuronal damage via inflammation in rat hippocampi., PMID:24007266

Up-regulation of RACK1 by TGF-β1 promotes hepatic fibrosis in mice., PMID:23555900

RACK1 identified as the PCBP1-interacting protein with a novel functional role on the regulation of human MOR gene expression., PMID:23173782

Identification and validation of new autoantibodies for the diagnosis of DCIS and node negative early-stage breast cancers., PMID:22886747

Overexpressions of RACK1 and CD147 associated with poor prognosis in stage T1 pulmonary adenocarcinoma., PMID:22592183

Tagging of functional ribosomes in living cells by HaloTag® technology., PMID:21082278

A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction., PMID:20196072

Expression of RACK1 is a novel biomarker in pulmonary adenocarcinomas., PMID:19892429

Epstein-Barr virus BART gene expression., PMID:19141439

mrtl-A translation/localization regulatory protein encoded within the human c-myc locus and distributed throughout the endoplasmic and nucleoplasmic reticular network., PMID:18816594

The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex., PMID:18172692

Characterization of IQGAP1-containing complexes in NK-like cells: evidence for Rac 2 and RACK1 association during homotypic adhesion., PMID:17269730

Cell-specific expression of the epm1 (cystatin B) gene in developing rat cerebellum., PMID:16137571

RACK1 mediates activation of JNK by protein kinase C [corrected]., PMID:16061178

Identification of the linker histone H1 as a protein kinase Cepsilon-binding protein in vascular smooth muscle., PMID:15499382

Identification and characterization of the human ARD1-NATH protein acetyltransferase complex., PMID:15496142

Aging-related alterations in the distribution of Ca(2+)-dependent PKC isoforms in rabbit hippocampus., PMID:15382255

Ki-1/57 interacts with RACK1 and is a substrate for the phosphorylation by phorbol 12-myristate 13-acetate-activated protein kinase C., PMID:14699138

Release of eIF6 (p27BBP) from the 60S subunit allows 80S ribosome assembly., PMID:14654845

Protein kinase C isoforms in the enteric nervous system., PMID:12811573

Measles virus suppresses interferon-alpha signaling pathway: suppression of Jak1 phosphorylation and association of viral accessory proteins, C and V, with interferon-alpha receptor complex., PMID:12620806

Effects of prenyl pyrophosphates on the binding of PKCgamma with RACK1., PMID:12506405

Activation of Integrin-RACK1/PKCalpha signalling in human articular chondrocyte mechanotransduction., PMID:12435334

New insights into the molecular basis of progressive myoclonus epilepsy: a multiprotein complex with cystatin B., PMID:12393805

The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins., PMID:12359736

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human RACK1 Polyclonal Antibody [PHF60301]
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