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Anti-OSTF1 Polyclonal Antibody

Catalog #:   PHJ34301 Specific References (9) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q92882
Overview

Catalog No.

PHJ34301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human OSTF1 (Gly12-Asp214).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Osteoclast-stimulating factor 1,OSTF1

Purification

Purified by antigen affinity column.

Accession

Q92882

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with OSTF1 antibody (PHJ34301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with OSTF1 antibody (PHJ34301) at 1 μg/ml.

    Lane 1: HEK293T
    Lane 2: HEK293T treated with the blocking peptide

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human duodenum stained for OSTF1 with PHJ34301.
References

Transcriptome and Proteome Analyses of TNFAIP8 Knockdown Cancer Cells Reveal New Insights into Molecular Determinants of Cell Survival and Tumor Progression., PMID:27807832

Synovial fluid proteome in rheumatoid arthritis., PMID:27274716

Common genetic denominators for Ca++-based skeleton in Metazoa: role of osteoclast-stimulating factor and of carbonic anhydrase in a calcareous sponge., PMID:22506035

Brain-derived neurotrophic factor is a potential osteoclast stimulating factor in multiple myeloma., PMID:21400510

A global proteomics approach identifies novel phosphorylated signaling proteins in GPVI-activated platelets: involvement of G6f, a novel platelet Grb2-binding membrane adapter., PMID:16941570

SH3P2 in complex with Cbl and Src., PMID:15135048

Eosinophil chemotactic factor-L (ECF-L): a novel osteoclast stimulating factor., PMID:12854845

Production of lymphotoxin, a bone-resorbing cytokine, by cultured human myeloma cells., PMID:3497347

Evidence for the secretion of an osteoclast stimulating factor in myeloma., PMID:4413338

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-OSTF1 Polyclonal Antibody [PHJ34301]
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