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Anti-NEU4 Polyclonal Antibody

Catalog #:   PHJ30401 Specific References (11) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q8WWR8
Overview

Catalog No.

PHJ30401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NEU4 (Tyr22-Pro286).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Sialidase-4,NEU4,N-acetyl-alpha-neuraminidase 4

Purification

Purified by antigen affinity column.

Accession

Q8WWR8

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NEU4 antibody (PHJ30401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
References

Increased sialidase activity in serum of cancer patients: Identification of sialidase and inhibitor activities in human serum., PMID:25652216

Molecular cloning and biochemical characterization of medaka (Oryzias latipes) lysosomal neu4 sialidase., PMID:24744226

A novel insulin receptor-signaling platform and its link to insulin resistance and type 2 diabetes., PMID:24583283

Expression of the infectious salmon anemia virus receptor on atlantic salmon endothelial cells correlates with the cell tropism of the virus., PMID:22811536

Sialidase NEU4 hydrolyzes polysialic acids of neural cell adhesion molecules and negatively regulates neurite formation by hippocampal neurons., PMID:22393058

Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Galphai proteins and matrix metalloproteinase-9., PMID:20213245

Human sialidase as a cancer marker., PMID:18651674

Monocyte differentiation up-regulates the expression of the lysosomal sialidase, Neu1, and triggers its targeting to the plasma membrane via major histocompatibility complex class II-positive compartments., PMID:16835219

Differential expression of endogenous sialidases of human monocytes during cellular differentiation into macrophages., PMID:15885103

Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family., PMID:14962670

Expression of Neural Antigens in Normal Xenopus Embryos and Induced Explants: (Xenopus laevis/neural antigens/monoclonal antibodies/neural induction/neural differentiation)., PMID:37280931

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-NEU4 Polyclonal Antibody [PHJ30401]
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