Catalog No.
MHC09003
Species reactivity
Human, Mouse, Rat
Host species
Mouse
Isotype
IgG2b
Clonality
Monoclonal
Immunogen
E. coli - derived recombinant Human GAPDH protein (Met1-Glu335).
Tested applications
ELISA: 1:2000-1:4000, WB: 1:1000-1:4000
Target
GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P04406
Applications
ELISA, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
1A200
Maternal transfer of anti-GAPDH IgG prevents neonatal infections caused by Staphylococcus aureus and group B Streptococcus., PMID:40241760
Induction of interleukin 21 receptor expression via enhanced intracellular metabolism in B cells and its relevance to the disease activity in systemic lupus erythematosus., PMID:39740932
Evaluating the role of active TGF-β1 as inflammatory biomarker in Kashmiri (North-Indian) patients with systemic sclerosis: a case-control study., PMID:39716300
A click chemistry-based biorthogonal approach for the detection and identification of protein lysine malonylation for osteoarthritis research., PMID:39713453
CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression., PMID:39689859
Correction to "Increased expression of desmin and vimentin reduces bladder smooth muscle contractility via JNK2"., PMID:39652015
Pioneering molecular screening for cervical precursor lesions and cervical cancer in sera., PMID:39610929
The immune responses elicited by six recombinant antigens of Mycoplasma hyopneumoniae in mice., PMID:39531743
Binding of glyceraldehyde-3-phosphate dehydrogenase to G-actin promotes the transnitrosylation reaction., PMID:39481743
Distinct Non-Human Leukocyte Antigen Antibody Signatures Correlate with Endothelial Crossmatch Status in Lung and Renal Transplant Recipients., PMID:39408890
FXR, MRP-1 and SLC7A5: New Targets for the Treatment of Hepatocellular Carcinoma., PMID:39194338
Cord blood methylation at TNFRSF17 is associated with early allergic phenotypes., PMID:39085570
Resolving the current controversy of use and reuse of housekeeping proteins in ageing research: Focus on saving people's tax dollars., PMID:39067773
Regulation of AMPK and GAPDH by Transglutaminase 2 Plays a Pivotal Role in Microvascular Leakage in Diabetic Retinas., PMID:39024127
A fungal-binding agglutinin in the skin slime of Japanese flounder (Paralichthys olivaceus) is glyceraldehyde 3-phosphate dehydrogenase., PMID:38923675
The rheumatoid arthritis drug auranofin exerts potent anti-lymphoma effect by stimulating TXNRD-mediated ROS generation and inhibition of energy metabolism., PMID:38909408
AMPK drives both glycolytic and oxidative metabolism in murine and human T cells during graft-versus-host disease., PMID:38810258
Label-Free Quantitative Analysis of Pig Liver Proteome after Hepatitis E Virus Infection., PMID:38543773
Autoimmune retinopathy in a patient with smoldering multiple myeloma: a case report., PMID:38363513
A real-time PCR for quantification of parasite burden and its correlations with clinical characteristics and anti-rKRP42 IgG level in cutaneous leishmaniasis in Sri Lanka., PMID:38341021
[Corrigendum] Long non‑coding RNA UCA1 confers tamoxifen resistance in breast cancer endocrinotherapy through regulation of the EZH2/p21 axis and the PI3K/AKT signaling pathway., PMID:38214398
Network analysis of H. pylori effect on AGS human gastric adenocarcinoma cells gene expression profile., PMID:40406433
An antibody-free evaluation of an mRNA COVID-19 vaccine., PMID:38096736
Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826
Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868
Comparative analyses of functional antibody-mediated inhibition with anti-circumsporozoite monoclonal antibodies against transgenic Plasmodium berghei., PMID:37936181
Autoimmune anti-D in an RhD-positive young infant: Learning from a rare case., PMID:37930933
Fluorescence-tagged salivary small extracellular vesicles as a nanotool in early diagnosis of Parkinson's disease., PMID:37667227
Evaluation of androgen receptor markers in erectile dysfunction., PMID:37551851
AMPK Drives Both Glycolytic and Oxidative Metabolism in T Cells During Graft-versus-host Disease., PMID:37398326
S-nitrosylation and S-glutathionylation of GAPDH: Similarities, differences, and relationships., PMID:37355052
Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model., PMID:37243109
Quantification of SARS-CoV-2 spike protein expression from mRNA vaccines using isotope dilution mass spectrometry., PMID:37202272
Hijacking of Host Plasminogen by Mesomycoplasma (Mycoplasma) hyopneumoniae via GAPDH: an Important Virulence Mechanism To Promote Adhesion and Extracellular Matrix Degradation., PMID:37199643
Performance benchmarking microplate-immunoassays for quantifying target-specific cysteine oxidation reveals their potential for understanding redox-regulation and oxidative stress., PMID:37192685
Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes., PMID:37189694
Activation of the toll-like receptor 2 signaling pathway by GAPDH from bacterial strain RD055328., PMID:37169920
VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation., PMID:37114564
Differential patterns of fish sensitization in Asian populations: Implication for precision diagnosis., PMID:37032258
Glyceraldehyde-3-phosphate dehydrogenase regulates activation of c-Jun N-terminal kinase under oxidative stress., PMID:36963174
The Application of Guanidinium to Improve Biomolecule Quality in Fixed, Paraffin-embedded Tissue., PMID:36869703
Molecular Characteristics of Toxicity of Acrolein Produced from Spermine., PMID:36830667
Establishment and Application of an Indirect ELISA for the Detection of Antibodies to Porcine Streptococcus suis Based on a Recombinant GMD Protein., PMID:36830506
Novel maternal autoantibodies in autism spectrum disorder: Implications for screening and diagnosis., PMID:36816132
Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray., PMID:36789694
Dynamic cycling with a unique Hsp90/Hsp70-dependent chaperone machinery and GAPDH is needed for heme insertion and activation of neuronal NO synthase., PMID:36596358
[Protective effect of heat shock transcription factor 1 on acute lung injury in septic rats by regulating NOD-like receptor protein 3 inflammasome activation]., PMID:36567560
Biglycan as a mediator of proinflammatory response and target for MDS and sAML therapy., PMID:36531688
Effect of FXR agonist GW4064 in the treatment of hilar cholangiocarcinoma in rats., PMID:36344586
Significance of Mannan Binding Lectin-Associated Serine Protease 2 in Urinary Extracellular Vesicles in IgA Nephropathy., PMID:36149051