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Anti-NFKBID Polyclonal Antibody

Catalog #:   PHJ26801 Specific References (8) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q8NI38
Overview

Catalog No.

PHJ26801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NFKBID (Asp46-Val285).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

NFKB inhibitor delta,IkB-delta,TA-NFKBH,NF-kappa-B inhibitor delta,IkappaBNS,I-kappa-B-delta,T-cell activation NFKB-like protein,IKBNS,IkappaBdelta,NFKBID

Purification

Purified by antigen affinity column.

Accession

Q8NI38

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NFKBID antibody (PHJ26801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with NFKBID antibody (PHJ26801) at 1 μg/ml.

    Lane 1: Hela
    Lane 2: MCF7

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Immunohistochemical
    Immunohistochemical analysis of Human colon carcinoma stained for NFKBID with PHJ26801.
References

Enhanced B Cell Receptor Signaling Partially Compensates for Impaired Toll-like Receptor 4 Responses in LPS-Stimulated IκBNS-Deficient B Cells., PMID:37174629

IκBNS expression in B cells is dispensable for IgG responses to T cell-dependent antigens., PMID:36341341

Nfkbid-mediated humoral immunity during secondary toxoplasmosis., PMID:35190281

Genetic mapping reveals Nfkbid as a central regulator of humoral immunity to Toxoplasma gondii., PMID:34871323

TACI expression and plasma cell differentiation are impaired in the absence of functional IκBNS., PMID:30597621

Heterozygous Mutation in IκBNS Leads to Reduced Levels of Natural IgM Antibodies and Impaired Responses to T-Independent Type 2 Antigens., PMID:26973645

B-1a transitional cells are phenotypically distinct and are lacking in mice deficient in IκBNS., PMID:25228759

A forward genetic screen reveals roles for Nfkbid, Zeb1, and Ruvbl2 in humoral immunity., PMID:22761313

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-NFKBID Polyclonal Antibody [PHJ26801]
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