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Anti-MIB1 Polyclonal Antibody

Catalog #:   PHJ17601 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q86YT6
Overview

Catalog No.

PHJ17601

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MIB1 (Arg769-Glu1000).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Zinc finger ZZ type with ankyrin repeat domain protein 2,MIB1,DIP-1,Mind bomb homolog 1,ZZANK2,DAPK-interacting protein 1,E3 ubiquitin-protein ligase MIB1,KIAA1323,DIP1,RING-type E3 ubiquitin transferase MIB1

Purification

Purified by antigen affinity column.

Accession

Q86YT6

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MIB1 antibody (PHJ17601) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
References

Congress of Neurological Surgeons systematic review and evidence based guideline on neuropathology for WHO grade II diffuse glioma: update., PMID:39747718

P53 and Ki67 Biomarkers are Predictors for Malignant Transformation in Oral Submucous Fibrosis: A Prospective Study., PMID:39733401

The combined assessment of p16INK4a and Mib/Ki-67 in oral squamous cell carcinoma., PMID:39664175

Comparison of MIB-1-Specific Membrane Staining in Hyalinising Trabecular Tumor Using Mainstream Automated Immunohistochemical Staining Platforms., PMID:39447085

Sjögren syndrome induced by anti PDL-1 treatment for TNBC: case report and review of literature., PMID:39434886

Flow cytometric analysis for Ki67 assessment in formalin-fixed paraffin-embedded breast cancer tissue., PMID:39183273

Immunohistochemical Profile of p62/SQSTM1/Sequestosome-1 in Human Low- and High-Grade Intracranial Meningiomas., PMID:39131899

POTENTIAL DIAGNOSTIC BIOMARKERS FOR HUMAN MESENCHYMAL TUMORS, ESPECIALLY LMP2/Β1I AND CYCLIN E1/MIB1 DIFFERENTIAL EXPRESSION: PRUM-IBIO STUDY., PMID:39089269

Meningioma Prognostic Tool Based on Correlation of Histopathological Grading and Immunohistochemistry., PMID:39055905

The expression of c-MYC, Cyclin D1 and Ki-67/MIB-1 in benign and malignant thyroid tissues: is there any diagnostic value?, PMID:39020541

[Plasmablastic lymphoma presenting with plasmacytosis and polyclonal hypergammopathy]., PMID:38448005

Development of a flow cytometric panel to assess prognostic biomarkers in fine needle aspirates of canine cutaneous or subcutaneous mast cell tumors., PMID:38076567

Voxel-based clustered imaging by multiparameter diffusion tensor images for predicting the grade and proliferative activity of meningioma., PMID:37644780

Comparison Between Manual and Automated Assessment of Ki-67 in Breast Carcinoma: Test of a Simple Method in Daily Practice., PMID:37559526

The Approach of Artificial Intelligence in Neuroendocrine Carcinomas of the Breast: A Next Step towards Precision Pathology?-A Case Report and Review of the Literature., PMID:37109630

Multiparametric magnetic resonance imaging for radiation therapy response monitoring in soft tissue sarcomas: a histology and MRI co-registration algorithm., PMID:37056570

Ki-67, topoisomerase IIα and miR-221 have a limited prostate cancer risk stratification ability on a medium-term follow-up: results of a high-risk radical prostatectomy cohort., PMID:36217395

Expression of Ki67 as detected by MIB-1 and its association with histopathological high-risk factors among patients with retinoblastoma tumour: a cross-sectional study., PMID:36161862

Systematically higher Ki67 scores on core biopsy samples compared to corresponding resection specimen in breast cancer: a multi-operator and multi-institutional study., PMID:35729220

Desmoplastic Small Round Cell Tumor of the Uterus: A Report of Molecularly Confirmed Case with EWSR1-WT1 Fusion., PMID:35626339

Spatial Metabolomics Identifies Distinct Tumor-Specific Subtypes in Gastric Cancer Patients., PMID:35395077

Establishment and Characterization of Brain Cancer Primary Cell Cultures From Patients to Enable Phenotypic Screening for New Drugs., PMID:35370679

Lymphoid hyperplasia with a polyp form of the gallbladder macroscopically mimicking carcinoma., PMID:35091990

Ketogenic Metabolic Therapy, Without Chemo or Radiation, for the Long-Term Management of IDH1-Mutant Glioblastoma: An 80-Month Follow-Up Case Report., PMID:34136522

Expression patterns for Bcl-2, EMA, β-catenin, E-cadherin, PAX8, and MIB1 in thymomas., PMID:34135467

A simple digital image analysis system for automated Ki67 assessment in primary breast cancer., PMID:33590538

UK NEQAS ICC & ISH Ki-67 Data Reveal Differences in Performance of Primary Antibody Clones., PMID:33337635

A juvenile case of epilepsy-associated, isocitrate dehydrogenase wild-type/histone 3 wild-type diffuse glioma with a rare BRAF A598T mutation., PMID:32996219

Myoepithelial Tumors of Salivary Gland: A Clinicopathologic and Immunohistochemical Study of 15 Patients with MIB-1 Correlation., PMID:32959208

A method to obtain reproducible Ki-67 indices in lung adenocarcinoma., PMID:32813926

Prediction of relapse in stage I testicular germ cell tumor patients on surveillance: investigation of biomarkers., PMID:32758242

Comparison of different anti-Ki67 antibody clones and hot-spot sizes for assessing proliferative index and grading in pancreatic neuroendocrine tumours using manual and image analysis., PMID:32617996

PSMD9 expression correlates with recurrence after radiotherapy in patients with cervical cancer., PMID:32565983

Preoperative denosumab treatment with curettage may be a risk factor for recurrence of giant cell tumor of bone., PMID:32539628

Performance analysis of the anti-Ki67 antibody clone 30-9 for immunohistochemical staining of breast cancer., PMID:32440959

Spontaneous pancreatic undifferentiated pleomorphic sarcoma in a laboratory rat: A case report., PMID:31773099

Correlation Between Digital and Manual Determinations of Ki-67/MIB-1 Proliferative Indices in Human Meningiomas., PMID:31771372

Impact of Primary Antibody Clone, Format, and Stainer Platform on Ki67 Proliferation Indices in Breast Carcinomas., PMID:31460869

CytoLyt fixation significantly inhibits MIB1 immunoreactivity whereas alternative Ki-67 clone 30-9 is not susceptible to the inhibition: Critical diagnostic implications., PMID:31398281

A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa., PMID:31281635

18F-Fluoroethyl-tyrosine uptake is correlated with amino acid transport and neovascularization in treatment-naive glioblastomas., PMID:31289907

Two ways to inactivate the Ki-67 protein-Fragmentation by nanoparticles, crosslinking with fluorescent dyes., PMID:31251462

Mindbomb Homolog-1 Index in the Prognosis of High-Grade Glioma and Its Clinicopathological Correlation., PMID:31001003

Subtypes of Breast Cancer in Lao P.D.R.: A Study in a Limited-Resource Setting., PMID:30806064

Angiogenic regeneration defines loco-regional recurrence following pre-operative radio-chemotherapy for rectal cancer: a pilot study., PMID:30721420

SMARCB1- and vimentin-positive esophageal carcinoma with undifferentiated components, rhabdoid features, and a good prognosis: a case report., PMID:30649642

LC3A, LC3B and Beclin-1 Expression in Gastric Cancer., PMID:30504397

Ki67 reproducibility using digital image analysis: an inter-platform and inter-operator study., PMID:30181553

The Combination Of Weak Expression Of PRDX4 And Very High MIB-1 Labelling Index Independently Predicts Shorter Disease-free Survival In Stage I Lung Adenocarcinoma., PMID:30013444

Expression and significance of Ki-67 in lung cancer., PMID:29940632

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-MIB1 Polyclonal Antibody [PHJ17601]
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