Catalog No.
PHD70101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human U2AF2 (Pro138-Gly338).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Splicing factor U2AF 65 kDa subunit, hU2AF65, hU2AF(65), U2 auxiliary factor 65 kDa subunit, U2AF65, U2AF2, U2 snRNP auxiliary factor large subunit
Purification
Purified by antigen affinity column.
Accession
P26368
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2., PMID:39495216
Splicing machinery is impaired in rheumatoid arthritis, associated with disease activity and modulated by anti-TNF therapy., PMID:34625402
Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome., PMID:25169209
Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer., PMID:22682314
Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors., PMID:17137510
Substrate-dependent differences in U2AF requirement for splicing in adenovirus-infected cell extracts., PMID:15899895
Dual function for U2AF(35) in AG-dependent pre-mRNA splicing., PMID:11604503
The role of U2AF35 and U2AF65 in enhancer-dependent splicing., PMID:11421359
Serologic responses in patients with malignant mesothelioma: evidence for both public and private specificities., PMID:10783126
Evidence for substrate-specific requirement of the splicing factor U2AF(35) and for its function after polypyrimidine tract recognition by U2AF(65)., PMID:10567551
Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor., PMID:10049361
Existence and localization of a protein in zebra finch brain with similar structural features as the large subunit of the splicing factor U2AF., PMID:9597763
Targeting of U2AF65 to sites of active splicing in the nucleus., PMID:9166400
Novel nuclear autoantigen with splicing factor motifs identified with antibody from hepatocellular carcinoma., PMID:8227358
For research use only. Not for human or drug use.
