Human sCD14 ELISA Kit

Catalog No.

KHC33701

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human sCD14 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human sCD14 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Human sCD14 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

sCD14

Applications

Used for the quantitative determination of Human sCD14 concentration in serum, plasma and cell culture supernatant.

Detection method

Colorimetric

Sample type

Serum, Plasma and Cell culture supernatant.

Assay type

Quantitative

Range

93.75 - 6,000 pg/mL

Sensitivity

27.26 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/mL)	2745.4	642.4	159.6	2800.2	747.4	161.7
Standard deviation	150.1	66.8	7.0	279.6	66.9	7.4
CV (%)	5.5	10.4	4.4	10.0	9.0	4.6

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14

Document Download

Human sCD14 ELISA Kit.pdf