Human sCD14 ELISA Kit

Catalog No.

KHC33701

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human sCD14 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human sCD14 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Human sCD14 is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

sCD14

Applications

Used for the quantitative determination of Human sCD14 concentration in serum, plasma and cell culture supernatant.

Detection method

Colorimetric

Sample type

Serum, Plasma and Cell culture supernatant.

Assay type

Quantitative

Range

93.75 - 6,000 pg/mL

Sensitivity

27.26 pg/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/mL)	2745.4	642.4	159.6	2800.2	747.4	161.7
Standard deviation	150.1	66.8	7.0	279.6	66.9	7.4
CV (%)	5.5	10.4	4.4	10.0	9.0	4.6

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14

Oral Plant-Derived Nanomedicines Mitigate Acetaminophen-Induced Liver Injury by Modulating the Gut-Liver Axis and Intestinal Microbiota Metabolism., PMID:40511710

CD14 plays a critical role in pain and inflammation across multiple models of post-traumatic osteoarthritis., PMID:40502022

5-HT7R Deficiency Alleviates ADP-Heptose-Induced Cognitive Impairment via Inhibiting Ferroptosis and Neuroinflammation in Mice., PMID:40501108

Endothelial CLEC5A drives barrier dysfunction and vascular leakage responsible for lung injury in bacterial pneumonia and sepsis., PMID:40498836

VPAC1 and VPAC2 Receptor Heterozygosity Confers Distinct Biological Properties to BV2 Microglial Cells., PMID:40497945

Inflammatory and vascular biomarkers as predictors of all-cause death and cardiovascular outcomes in an Australian community-based cohort., PMID:40495286

Naproxen and prednisolone reduced intestinal alteration and permeability and bacterial translocation in rat adjuvant-induced arthritis., PMID:40493287

Causal association between immune cytokines and psoriasis: A two-way Mendelian randomization study., PMID:40489870

A novel LRR domain-containing C-type lectin gene with immune regulatory functions protects Penaeus vannamei against Vibrio parahaemolyticus infection., PMID:40484067

Characterization of immune cells in the rat intestinal mucosa and liver involved in inflammation caused by LPS and evaluation of the effects of N-acetylcysteine and disulfiram (well-known sulfur drugs) for this inflammation., PMID:40482471

Inflammation, antiretroviral therapy continuation, and HIV disease progression in postpartum women with HIV., PMID:40476693

Activation of Human FPR2 with AT-RvD1 Resolves Acute Sialadenitis in Vivo., PMID:40464830

FCGR1A Alleviates Ischemic Stroke-induced Injury by Promoting Anti-Inflammatory Microglial Polarization via the AMPK-mTOR Signaling Pathway., PMID:40464505

Comparison of oral and gut microbiome highlights role of oral bacteria in systemic inflammation in HIV., PMID:40463026

Anti-CD14 treatment in patients with severe COVID-19: Clinical and biological effects in a Phase 2 randomized open-label adaptive platform clinical trial., PMID:40462830

Bacteriophage cocktail LEC2-LEC10 for broad-spectrum control of pathogenic and uncharacterized Escherichia coli in fresh produce., PMID:40458707

TLR4 and prostaglandin pathways at the crossroads of endotoxemia-induced lipolysis., PMID:40458405

Liraglutide modulates cyclooxygenase and α7 acetylcholine receptors: in vitro and in silico insights into its anti-inflammatory role in LPS-induced inflammation in RAW 264.7 macrophages., PMID:40448826

Single-cell analyses identify monocyte gene expression profiles that influence HIV-1 reservoir size in acutely treated cohorts., PMID:40442100

Prenatal Exposure to Lipopolysaccharide or Valproate Leads to Abnormal Accumulation of the NMDA Receptor Agonist D-Aspartate in the Adolescent Rat Brain., PMID:40437860

Preoptic EP3R neurons constitute a two-way switch for fever and torpor., PMID:40437091

Biomarkers of intestinal permeability in major psychiatric disorders: Distinct biological roles call for a more nuanced application., PMID:40436362

Zinc Supplementation, Inflammation, and Gut Integrity Markers in HIV Infection: A Randomized Placebo-Controlled Trial., PMID:40431411

Persistent Monocytic Bioenergetic Impairment and Mitochondrial DNA Damage in PASC Patients with Cardiovascular Complications., PMID:40429707

Elucidating the Antidepressant-Like Effect of Tannic Acid in Mice: Modulation of Serotonergic System and Pro-Inflammatory Mediators., PMID:40425908

Interactions of Probiotics with the Immune Cells of Patients with COVID-19 Pneumonia., PMID:40424990

Highly elevated sepsis biomarkers in advanced cholangiocarcinoma without sepsis: A case report and literature review., PMID:40419900

Urotensin-II receptor contributes to the pro-inflammatory TLR4/MyD88/NF-κB/iNOS/NO pathway-mediated cardiovascular response to systemic lipopolysaccharide challenge in a septic shock model in rats., PMID:40418546

Presepsin in the amniotic fluid is a useful clinical inflammatory biomarker for the prenatal predictive diagnosis of histologic chorioamnionitis: a retrospective preliminary study., PMID:40413908

Tn antigen suppresses lipopolysaccharide-induced dermatitis via Clec10a., PMID:40412372

Understanding residual risk of cardiovascular disease in people with HIV., PMID:40397567

Ex vivo lung perfusion with GLP-1R agonist mitigates ischemia/reperfusion injury through pyroptosis modulation in lung transplantation- an experimental study., PMID:40387728

Effects of Porphyromonas gingivalis lipopolysaccharide on the behavior of human dental pulp stem cells in vitro and their inflammation-related transcriptomics profile., PMID:40381466

Prospective evaluation of soluble CD14 as a biomarker following five aflibercept treatments in diabetic macular edema., PMID:40379891

Sex-dependent changes induced by combined low-level systemic inflammation and chronic mild unpredictable stress in rats are partially attenuated by positive modulation of α5 GABAA receptors., PMID:40379031

Tryptophan-kynurenine metabolites associate with inflammation and immunologic phenotypes in common variable immunodeficiency., PMID:40378971

Gastrointestinal Barrier Disruption in Post-COVID Syndrome Fatigue Patients., PMID:40372110

Single-cell transcriptome reveals potential mechanisms for gout in children., PMID:40370447

Cell-Autonomous Immunity: From Cytosolic Sensing to Self-Defense., PMID:40362284

Large B-cell lymphoma imprints a dysfunctional immune phenotype that persists years after treatment., PMID:40359478

Study of some biological markers in cord blood of preterm and term infants and their association with neonatal sepsis., PMID:40353384

Investigating endogenous immune-mediated monocyte memory in rheumatoid arthritis., PMID:40350372

Impaired LPS tolerance in monocytes of coronary atherosclerosis patients is associated with the intermediate subset., PMID:40350260

Palmitic acid and lipopolysaccharide induce macrophage TNFα secretion, suppressing browning regulators and mitochondrial respiration in adipocytes., PMID:40348028

Dynamics of antimicrobial proteins' expression and their bactericidal activity in mouse milk., PMID:40345232

scRNA-seq and scATAC-seq analyses highlight the role of TNF signaling pathway in chronic obstructive pulmonary disease model mice., PMID:40343927

MCTR1 ameliorates LPS-induced lung injury by inhibiting neutrophil reverse transendothelial migration., PMID:40339497

T-lymphocytes suppression by CD14+ monocytes with high expression of ULK2 in patients with multiple myeloma., PMID:40336101

The NLRP3 inflammasome in microglia regulates repetitive behavior by modulating NMDA glutamate receptor functions., PMID:40333183

Sialyllactose Attenuates Inflammation and Injury of Intestinal Epithelial Cells upon Enterotoxigenic Escherichia coli Infection., PMID:40332525

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Human sCD14 ELISA Kit.pdf