Catalog No.
YHN30601
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gln14-Lys126
Predicted molecular weight
15.16 kDa
Nature
Recombinant
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q96EP1
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
E3 ubiquitin-protein ligase CHFR, RNF196, Checkpoint with forkhead and RING finger domains protein, CHFR, RING-type E3 ubiquitin transferase CHFR, RING finger protein 196
Note
For research use only.
Evaluation of the acceptability of improved supplementary foods for the treatment of moderate acute malnutrition in Burkina Faso using a mixed method approach., PMID:26752599
Arsenic trioxide induces cell cycle arrest and alters DNA methylation patterns of cell cycle regulatory genes in colorectal cancer cells., PMID:27769816
CHFR negatively regulates SIRT1 activity upon oxidative stress., PMID:27883020
SMARCB1/INI1-deficient sinonasal carcinoma shows methylation of RASSF1 gene: A clinicopathological, immunohistochemical and molecular genetic study of a recently described entity., PMID:28069272
Sustained expression of miR-26a promotes chromosomal instability and tumorigenesis through regulation of CHFR., PMID:28126920
Phase II study of nab-paclitaxel in refractory small bowel adenocarcinoma and CpG island methylator phenotype (CIMP)-high colorectal cancer., PMID:29069279
Methylation-independent CHFR expression is a potential biomarker affecting prognosis in acute myeloid leukemia., PMID:29115660
Platform-independent gene expression signature differentiates sessile serrated adenomas/polyps and hyperplastic polyps of the colon., PMID:29284484
Analysis of DNA methylation and microRNA expression in NUT (nuclear protein in testis) midline carcinoma of the sinonasal tract: a clinicopathological, immunohistochemical and molecular genetic study., PMID:29322795
Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers., PMID:29344247
The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer., PMID:29367628
Drosophila bendless catalyzes K63-linked polyubiquitination and is involved in the response to DNA damage., PMID:29518634
Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells., PMID:30060952
Association of CHFR Promoter Methylation with Treatment Outcomes of Irinotecan-Based Chemotherapy in Metastatic Colorectal Cancer., PMID:30562637
Spatiotemporal regulation of the Dma1-mediated mitotic checkpoint coordinates mitosis with cytokinesis., PMID:30600396
CHFR promotes the migration of human gastric cancer cells by inducing epithelial-to-mesenchymal transition in a HDAC1-dependent manner., PMID:30799937
Promoter hypermethylation in ductal carcinoma in situ of the male breast., PMID:30921768
DNA methylation and hydroxymethylation patterns in acute myeloid leukemia patients with mutations in DNMT3A and IDH1/2 and their combinations., PMID:30988238
Antileukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia., PMID:31227645
Poly(ADP‑ribose) polymerase 1/2 inhibitors decrease the ubiquitination of ALC1 mediated by CHFR in breast cancer., PMID:31322269
MORC2 regulates DNA damage response through a PARP1-dependent pathway., PMID:31616951
Case Study: Landscape of Chimeric RNAs in Bladder Cancer., PMID:31728975
Identification of Subtypes of Barrett's Esophagus and Esophageal Adenocarcinoma Based on DNA Methylation Profiles and Integration of Transcriptome and Genome Data., PMID:32032585
Circ_CHFR expedites cell growth, migration and inflammation in ox-LDL-treated human vascular smooth muscle cells via the miR-214-3p/Wnt3/β-catenin pathway., PMID:32271446
Nutlin-3a suppresses poly (ADP-ribose) polymerase 1 by mechanisms different from conventional PARP1 suppressors in a human breast cancer cell line., PMID:32405340
Methylation patterns in dysplasia in inflammatory bowel disease patients., PMID:32456486
Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells., PMID:32466334
Human papillomavirus-positivity is associated with EREG down-regulation and promoter hypermethylation in head and neck squamous cell carcinoma., PMID:33007298
Kaposi's sarcoma-associated herpesvirus processivity factor (PF-8) recruits cellular E3 ubiquitin ligase CHFR to promote PARP1 degradation and lytic replication., PMID:33508027
A phase 2 trial of gemcitabine and docetaxel in patients with metastatic colorectal adenocarcinoma with methylated checkpoint with forkhead and ring finger domain promoter and/or microsatellite instability phenotype., PMID:33811727
CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells., PMID:33880594
Circ_CHFR Promotes Platelet-Derived Growth Factor-BB-Induced Proliferation, Invasion, and Migration in Vascular Smooth Muscle Cells via the miR-149-5p/NRP2 Axis., PMID:33990513
Vaccinia-related kinase 2 drives pancreatic cancer progression by protecting Plk1 from Chfr-mediated degradation., PMID:34140642
Cytosolic GDH1 degradation restricts protein synthesis to sustain tumor cell survival following amino acid deprivation., PMID:34269483
CHFR-mediated degradation of RNF126 confers sensitivity to PARP inhibitors in triple-negative breast cancer cells., PMID:34388456
CHFR regulates chemoresistance in triple-negative breast cancer through destabilizing ZEB1., PMID:34462429
Effect of MAP3K8 on Prognosis and Tumor-Related Inflammation in Renal Clear Cell Carcinoma., PMID:34567061
Circ-CHFR modulates the proliferation, migration, and invasion of ox-LDL-induced human aorta vascular smooth muscle cells through the miR-214-3p/PAPPA axis., PMID:34842180
CHFR and Paclitaxel Sensitivity of Ovarian Cancer., PMID:34885153
CHFR-Promoter-Methylation Status Is Predictive of Response to Irinotecan-based Systemic Chemotherapy in Advanced Colorectal Cancer., PMID:35093868
Organoid screening reveals epigenetic vulnerabilities in human colorectal cancer., PMID:35273398
WHO grading system for invasive pulmonary lung adenocarcinoma reveals distinct molecular signature: An analysis from the cancer genome atlas database., PMID:35339455
CHFR promotes metastasis of human gastric carcinoma by activating AKT and ERK via NRF2- ROS axis., PMID:37024798
Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions., PMID:37852964
The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation., PMID:38272222
Prognostic utility of circulating tumor DNA methylation analysis in stage IV colorectal cancer., PMID:39155651
Deep learning prioritizes cancer mutations that alter protein nucleocytoplasmic shuttling to drive tumorigenesis., PMID:40087285
Circulating Tumor DNA Methylation Is a Biomarker of Poor Recurrence-free Survival in Locally-Advanced Rectal Cancer., PMID:40155013
DNMT1 is required for efficient DSB repair and maintenance of replication fork stability, and its loss reverses resistance to PARP inhibitors in cancer cells., PMID:40234721
NUDT16 enhances the resistance of cancer cells to DNA-damaging agents by regulating replication fork stability via reversing HMGA1 ADP-ribosylation., PMID:40288645
For research use only. Not for human or drug use.
