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Anti-CRY1 Polyclonal Antibody

Catalog #:   PHH27601 Specific References (29) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q16526
Overview

Catalog No.

PHH27601

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CRY1 (Val3-Leu132).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Cryptochrome-1,CRY1,PHLL1

Purification

Purified by antigen affinity column.

Accession

Q16526

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CRY1 antibody (PHH27601) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
  • Immunocytochemistry/ Immunofluorescence
    CRY1 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CRY1 (PHH27601) at 11 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Full-Length Cryptochrome 1 in the Outer Segments of the Retinal Blue Cone Photoreceptors in Humans and Great Apes Suggests a Role Beyond Transcriptional Repression., PMID:40277221

Synergism of Cry1 Toxins by a Fusion Protein Derived from a Cadherin Fragment and an Antibody Peptide., PMID:39189874

Rational design and application of broad-spectrum antibodies for Bt Cry toxins determination., PMID:38843975

Establishment of novel receptor-antibody sandwich assays to broadly detect Bacillus thuringiensis Cry1 and Cry2 toxins., PMID:37972832

Circadian clock disruption in autoimmune thyroiditis., PMID:37548297

Cryptochrome proteins regulate the circadian intracellular behavior and localization of PER2 in mouse suprachiasmatic nucleus neurons., PMID:35046033

Antibodies Against the Clock Proteins Period and Cryptochrome Reveal the Neuronal Organization of the Circadian Clock in the Pea Aphid., PMID:34366893

Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library., PMID:29484477

Circadian clock cryptochrome proteins regulate autoimmunity., PMID:29109286

Broad specificity immunoassay for detection of Bacillus thuringiensis Cry toxins through engineering of a single chain variable fragment with mutagenesis and screening., PMID:28939515

Site-saturation mutagenesis library construction and screening for specific broad-spectrum single-domain antibodies against multiple Cry1 toxins., PMID:28601895

Simultaneous production of monoclonal antibodies against Bacillus thuringiensis (Bt) Cry1 toxins using a mixture immunization., PMID:28527908

Cyclic Variation of Cellular Clock Proteins in the Mouse Estrous Ovary., PMID:27920997

Interactive Organization of the Circadian Core Regulators PER2, BMAL1, CLOCK and PML., PMID:27383066

Production and Characterization of Monoclonal Antibody Broadly Recognizing Cry1 Toxins by Use of Designed Polypeptide as Hapten., PMID:27341419

Investigations of immunogenic, allergenic and adjuvant properties of Cry1Ab protein after intragastric exposure in a food allergy model in mice., PMID:27141950

Cryptochrome 1 in Retinal Cone Photoreceptors Suggests a Novel Functional Role in Mammals., PMID:26898837

A high-throughput liquid bead array-based screening technology for Bt presence in GMO manipulation., PMID:26499065

Surrogate molecular markers for IGHV mutational status in chronic lymphocytic leukemia for predicting time to first treatment., PMID:26038121

Role of type II protein arginine methyltransferase 5 in the regulation of Circadian Per1 gene., PMID:23133559

Light-dependent structural change of chicken retinal Cryptochrome4., PMID:23095750

cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis., PMID:22855128

Melatonin attenuates clock gene cryptochrome1, which may aggravate mouse anti-type II collagen antibody-induced arthritis., PMID:21113809

Analysis of chronic lymphotic leukemia transcriptomic profile: differences between molecular subgroups., PMID:19127482

The photopigment melanopsin is exclusively present in pituitary adenylate cyclase-activating polypeptide-containing retinal ganglion cells of the retinohypothalamic tract., PMID:11756521

Probable identification of a membrane-associated repressor of Bacillus subtilis DNA replication as the E2 subunit of the pyruvate dehydrogenase complex., PMID:10735853

Nuclear localization of the Arabidopsis blue light receptor cryptochrome 2., PMID:10476076

Characterization of photolyase/blue-light receptor homologs in mouse and human cells., PMID:9801304

The use of a ribosomal RNA targeted oligonucleotide probe for fluorescent labelling of viable Cryptosporidium parvum oocysts., PMID:9750273

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CRY1 Polyclonal Antibody [PHH27601]
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