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Anti-SMAD1 Polyclonal Antibody

Catalog #:   PHH20101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q15797
Overview

Catalog No.

PHH20101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SMAD1 (Asp259-Ser462).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

BSP1,Mothers against DPP homolog 1,Mothers against decapentaplegic homolog 1,SMAD1,Transforming growth factor-beta-signaling protein 1,MADH1,JV4-1,MADR1,SMAD family member 1,hSMAD1,SMAD 1,MAD homolog 1,Mad-related protein 1,BSP-1,Smad1

Purification

Purified by antigen affinity column.

Accession

Q15797

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SMAD1 antibody (PHH20101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

ActRII or BMPR ligands inhibit skeletal myoblast differentiation, and BMPs promote heterotopic ossification in skeletal muscles in mice., PMID:39994804

A Role for Leucine-Rich α2-Glycoprotein in Leukocyte Trafficking and Mucosal Inflammation in Inflammatory Bowel Disease., PMID:39918378

A Recombinant Antibody Against ALK2 Promotes Tissue Iron Redistribution and Contributes to Anemia Resolution in a Mouse Model of Anemia of Inflammation., PMID:39791515

A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling., PMID:39196179

Zinc transporter ZnT5 is associated with epithelial mesenchymal transition via SMAD1 in breast cancer., PMID:39138630

Targeting ONECUT2 inhibits tumor angiogenesis via down-regulating ZKSCAN3/VEGFA., PMID:38797268

Hedgehog Signaling Controls Chondrogenesis and Ectopic Bone Formation via the Yap-Ihh Axis., PMID:38540766

Endoglin, a Novel Biomarker and Therapeutical Target to Prevent Malignant Peripheral Nerve Sheath Tumor Growth and Metastasis., PMID:37432984

Endoglin and squamous cell carcinomas., PMID:37396898

Loss of AID exacerbates the malignant progression of CLL., PMID:36042317

Mechanical Stretch Induced Osteogenesis on Human Annulus Fibrosus Cells through Upregulation of BMP-2/6 Heterodimer and Activation of P38 and SMAD1/5/8 Signaling Pathways., PMID:36010676

Curcuminoids Inhibit Angiogenic Behaviors of Human Umbilical Vein Endothelial Cells via Endoglin/Smad1 Signaling., PMID:35409247

eNAMPT Is a Novel Damage-associated Molecular Pattern Protein That Contributes to the Severity of Radiation-induced Lung Fibrosis., PMID:35167418

Identification of Growth Factors, Cytokines and Mediators Regulated by Artemisia annua L. Polyphenols (pKAL) in HCT116 Colorectal Cancer Cells: TGF-β1 and NGF-β Attenuate pKAL-Induced Anticancer Effects via NF-κB p65 Upregulation., PMID:35163520

Prostate tumor-induced stromal reprogramming generates Tenascin C that promotes prostate cancer metastasis through YAP/TAZ inhibition., PMID:34845375

Nerve growth factor promotes osteogenic differentiation of MC3T3-E1 cells via BMP-2/Smads pathway., PMID:34391912

Specific Blockade of Bone Morphogenetic Protein-2/4 Induces Oligodendrogenesis and Remyelination in Demyelinating Disorders., PMID:34159538

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p., PMID:34158869

Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival., PMID:34013359

Up-regulation of miR-155 potentiates CD34+ CML stem/progenitor cells to escape from the growth-inhibitory effects of TGF-ß1 and BMP signaling., PMID:33907541

Bone morphogenetic protein 7 promotes resistance to immunotherapy., PMID:32973129

Maladjustment of β-CGRP/α-CGRP Regulation of AQP5 Promotes Transition of Alveolar Epithelial Cell Apoptosis to Pulmonary Fibrosis., PMID:32746702

Bevacizumab promotes active biological behaviors of human umbilical vein endothelial cells by activating TGFβ1 pathways via off-VEGF signaling., PMID:32587778

IFNβ signaling inhibits osteogenesis in human SLE bone marrow., PMID:32515653

Disruption of BMP Signaling Prevents Hyperthyroidism-Induced Bone Loss in Male Mice., PMID:32453466

Tumor necrosis factor-related apoptosis-inducing ligand regulate the accumulation of extracelluar matrix in pulmonary artery by activating the phosphorylation of Smad2/3., PMID:32233583

TGFβ-mediated expression of TGFβ-activating integrins in SSc monocytes: disturbed activation of latent TGFβ?, PMID:32143707

Smad1/5 signal transduction regulates the ameloblastic differentiation of induced pluripotent stem cells., PMID:32022225

Epigallocatechin-3-gallate inhibits tumor angiogenesis: involvement of endoglin/Smad1 signaling in human umbilical vein endothelium cells., PMID:31586903

Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections., PMID:30414134

Inhibitory Antibodies against Activin A and TGF-β Reduce Self-Supported, but Not Soluble Factors-Induced Growth of Human Pulmonary Arterial Vascular Smooth Muscle Cells in Pulmonary Arterial Hypertension., PMID:30274147

Erythroferrone inhibits the induction of hepcidin by BMP6., PMID:30097509

Therapeutic antibody directed osteogenic differentiation of induced pluripotent stem cell derived MSCs., PMID:29778895

Inhibin Is a Novel Paracrine Factor for Tumor Angiogenesis and Metastasis., PMID:29535220

Urinary IgG4 and Smad1 Are Specific Biomarkers for Renal Structural and Functional Changes in Early Stages of Diabetic Nephropathy., PMID:29490904

MicroRNA-31 is required for astrocyte specification., PMID:29380422

Activin A amplifies dysregulated BMP signaling and induces chondro-osseous differentiation of primary connective tissue progenitor cells in patients with fibrodysplasia ossificans progressiva (FOP)., PMID:29170109

Inhibition of BMP signaling overcomes acquired resistance to cetuximab in oral squamous cell carcinomas., PMID:29154973

Dynamic regulation of canonical TGFβ signalling by endothelial transcription factor ERG protects from liver fibrogenesis., PMID:29026072

The protective effects of bone morphogenetic protein-7 against epithelial injury and matrix metalloproteases upregulation induced by silica in vitro., PMID:28838258

Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6., PMID:28815688

BMP7 mediates the anticancer effect of honokiol by upregulating p53 in HCT116 cells., PMID:28731124

Transforming growth factor-β stimulates Smad1/5 signaling in pulmonary artery smooth muscle cells and fibroblasts of the newborn mouse through ALK1., PMID:28642261

BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5., PMID:28489883

Activation of CD137 Signaling Promotes Angiogenesis in Atherosclerosis via Modulating Endothelial Smad1/5-NFATc1 Pathway., PMID:28288971

Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia., PMID:28202082

Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma., PMID:28199984

Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein., PMID:28067796

Overexpression of colorectal cancer oncogene CHRDL2 predicts a poor prognosis., PMID:28009989

Follicle-Stimulating Hormone β-Subunit Potentiates Bone Morphogenetic Protein 9-Induced Osteogenic Differentiation in Mouse Embryonic Fibroblasts., PMID:27996168

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-SMAD1 Polyclonal Antibody [PHH20101]
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