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Anti-PRKD1 Polyclonal Antibody

Catalog #:   PHH03101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q15139
Overview

Catalog No.

PHH03101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PRKD1 (Thr651-Gln876).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PRKD1,Protein kinase D,nPKC-D1,Protein kinase C mu type,PKD1,PKD,nPKC-mu,PRKCM,Serine/threonine-protein kinase D1

Purification

Purified by antigen affinity column.

Accession

Q15139

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PRKD1 antibody (PHH03101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 29 kDa
    Observed MW: 29 kDa
References

Protein kinase D drives the secretion of invasion mediators in triple-negative breast cancer cell lines., PMID:38323010

A protein kinase D inhibitor suppresses AKT on T cells and antagonizes cancer immunotherapy by anti-PD-1., PMID:35849090

Pkd1 Mutation Has No Apparent Effects on Peroxisome Structure or Lipid Metabolism., PMID:35372986

Modulation of Titin-Based Stiffness in Hypertrophic Cardiomyopathy via Protein Kinase D., PMID:32351396

Angiogenin regulates PKD activation and COX-2 expression induced by TNF-α and bradykinin in the colonic myofibroblast., PMID:32171525

Ameliorating effects of Gö6976, a pharmacological agent that inhibits protein kinase D, on collagen-induced arthritis., PMID:31809526

Twist1-Induced Epithelial Dissemination Requires Prkd1 Signaling., PMID:31676574

Src-mediated tyrosine phosphorylation of Protein Kinase D2 at focal adhesions regulates cell adhesion., PMID:28842658

Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to N-cadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1., PMID:28056869

Bradykinin stimulates protein kinase D-mediated colonic myofibroblast migration via cyclooxygenase-2 and heat shock protein 27., PMID:28032559

Stress-dependent phosphorylation of myocardin-related transcription factor A (MRTF-A) by the p38(MAPK)/MK2 axis., PMID:27492266

Positive cross talk between protein kinase D and β-catenin in intestinal epithelial cells: impact on β-catenin nuclear localization and phosphorylation at Ser552., PMID:26739494

Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation., PMID:26324445

[Expression, purification and characterization of catalytic domain of protein kinase D1 in baculovirus-insect cell expression system]., PMID:25423759

Quantitative phosphoproteomics of cytotoxic T cells to reveal protein kinase d 2 regulated networks., PMID:25266776

Elevated protein kinase D3 (PKD3) expression supports proliferation of triple-negative breast cancer cells and contributes to mTORC1-S6K1 pathway activation., PMID:24337579

Expression of neuronal protein Kidins220/ARMS in the spleen and peripheral blood of mice following airway allergen challenge., PMID:24126542

PKD1 mediates negative feedback of PI3K/Akt activation in response to G protein-coupled receptors., PMID:24039875

A novel Lyn-protein kinase Cδ/ε-protein kinase D axis is activated in B cells by signalosome-independent alternate pathway BCR signaling., PMID:23457006

Protein kinase C regulates bombesin-induced rapid VEGF secretion in neuroblastoma cells., PMID:23155231

Rapid protein kinase D1 signaling promotes migration of intestinal epithelial cells., PMID:22595992

PKCβII modulation of myocyte contractile performance., PMID:22587992

Glucose transporter 2 expression is down regulated following P2X7 activation in enterocytes., PMID:22566162

Protein kinase D isoforms are dispensable for integrin-mediated lymphocyte adhesion and homing to lymphoid tissues., PMID:22311617

Protein kinase D1 maintains the epithelial phenotype by inducing a DNA-bound, inactive SNAI1 transcriptional repressor complex., PMID:22276203

Profiling of autoantibodies in IgA nephropathy, an integrative antibiomics approach., PMID:22157707

Crosstalk between adenosine A1 and β1-adrenergic receptors regulates translocation of PKCε in isolated rat cardiomyocytes., PMID:22105697

A novel protein kinase C target site in protein kinase D is phosphorylated in response to signals for cardiac hypertrophy., PMID:21726532

Discovery of cellular substrates for protein kinase A using a peptide array screening protocol., PMID:21644927

A novel protein kinase D phosphorylation site in the tumor suppressor Rab interactor 1 is critical for coordination of cell migration., PMID:21209314

Evidence for the phosphorylation of serine259 of histone deacetylase 5 by protein kinase Cδ., PMID:21146494

Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes., PMID:21132013

The tumor suppressor protein DLC1 is regulated by PKD-mediated GAP domain phosphorylation., PMID:21087603

NGF/TrkA-mediated Kidins220/ARMS signaling activated in the allergic airway challenge in mice., PMID:20934630

Protein kinase D-mediated phosphorylation of polycystin-2 (TRPP2) is essential for its effects on cell growth and calcium channel activity., PMID:20881056

Unique functions for protein kinase D1 and protein kinase D2 in mammalian cells., PMID:20819079

A role for PKD1 and PKD3 activation in modulation of calcium oscillations induced by orexin receptor 1 stimulation., PMID:20621130

Small interfering RNA screen for phosphatases involved in IgE-mediated mast cell degranulation., PMID:20483767

Regulation of oxysterol-binding protein Golgi localization through protein kinase D-mediated phosphorylation., PMID:20444975

Functional links between diacylglycerol and phosphatidylinositol signaling systems in human leukocyte-derived cell lines., PMID:19896467

Protein kinase D isoforms are expressed in rat and mouse primary sensory neurons and are activated by agonists of protease-activated receptor 2., PMID:19575452

Heterologous expression of the lipid transfer protein CERT increases therapeutic protein productivity of mammalian cells., PMID:19428735

A Golgi PKD activity reporter reveals a crucial role of PKD in nocodazole-induced Golgi dispersal., PMID:19416469

Vascular endothelial growth factor induces protein kinase D-dependent production of proinflammatory cytokines in endothelial cells., PMID:19176759

A protein kinase Cdelta-dependent protein kinase D pathway modulates ERK1/2 and JNK1/2 phosphorylation and Bim-associated apoptosis by asbestos., PMID:19116364

Characterization of cortactin as an in vivo protein kinase D substrate: interdependence of sites and potentiation by Src., PMID:19038333

Bryostatin 1 modulates beta-catenin subcellular localization and transcription activity through protein kinase D1 activation., PMID:18765827

Expression patterns of protein kinase D 3 during mouse development., PMID:18439271

Sequential protein kinase C (PKC)-dependent and PKC-independent protein kinase D catalytic activation via Gq-coupled receptors: differential regulation of activation loop Ser(744) and Ser(748) phosphorylation., PMID:18337243

Activation of protein kinase D1 in mast cells in response to innate, adaptive, and growth factor signals., PMID:18025234

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-PRKD1 Polyclonal Antibody [PHH03101]
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