Catalog No.
KDB94405
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human TNFa has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Etanercept in the sample competitively binds to the pre-coated protein with biotin-labeled Etanercept. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Etanercept bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Etanercept concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
31.25 - 2,000 ng/mL
Sensitivity
30.07 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (ng/mL)
|
872.9
|
227.2
|
57.2
|
804.4
|
212.7
|
48.6
|
|
Standard deviation
|
69.8
|
8.4
|
8.2
|
41.7
|
8.8
|
9.0
|
|
CV (%)
|
8.0
|
3.7
|
14.4
|
5.2
|
4.1
|
18.5
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
CAS: 185243-69-0
Comparison and analysis of biological drug consumption in two Italian hospital settings: Governance actions and prescribing appropriateness., PMID:40494065
Total Knee Arthroplasty in a Patient With Isolated Rheumatoid Arthritis of the Knee., PMID:40491655
Drug survival, long-term safety and efficacy of anti-TNF-alpha biosimilars: a monocentric retrospective study., PMID:40485573
Pharmacogenomics of TNF inhibitors., PMID:40469293
Evaluation of Factors Associated With Uveitis Onset Timing in Oligoarticular Juvenile Idiopathic Arthritis., PMID:40454975
Etanercept, a peripherally restricted TNF inhibitor, enhances cocaine-induced locomotor behaviors in male, but not female, rats., PMID:40446442
Psoriasis complicated with polymyositis successfully treated with Ixekizumab: A case report., PMID:40441209
Systematic review of comparative studies on emerging psoriasis treatments: comparing biologics with biologics, small molecule inhibitors with small molecule inhibitors, and biologics with small molecule inhibitors., PMID:40439875
Predicting Clinical Response to Monoclonal TNF Inhibitors in Rheumatoid Arthritis: A Transcriptomic Approach Based on Transmembrane TNF Reverse Signaling and Nrf2 Activation., PMID:40428231
Comparative efficacy, safety and immunogenicity of biosimilars and their reference biologic drugs in ankylosing spondylitis: a systematic review and meta-analysis of randomized controlled trials., PMID:40418343
The Relationship Between TNF-α Inhibitor Potency and HBV Reactivation in Patients With Rheumatic Disorders., PMID:40418092
Rapid baseline hump-free analysis of therapeutic proteins in a wide molecular weight range by SDS - capillary agarose gel electrophoresis., PMID:40409905
Coexistent Ankylosing Spondylitis and Ocular Toxocariasis in a Pediatric Patient Manifesting As Bilateral Panuveitis., PMID:40406765
Evaluating changes in baseline characteristics and drug utilisation pattern in patients with moderate-to-severe psoriasis: findings from the British Association of Dermatologists Biologics and Immunomodulators Register (BADBIR) Cohort., PMID:40402160
Dual therapy for amanita phalloides-induced acute liver failure in mice: A combination of etanercept and alpha-1 antitrypsin., PMID:40398510
Systematic review and meta-analysis of the efficacy of biologic and targeted synthetic therapies in sarcoidosis., PMID:40393718
Identification of therapeutic targets for neonatal respiratory distress: A systematic druggable genome-wide Mendelian randomization., PMID:40388790
Risk of adverse events of psoriasis treatment with biologic agents and new small molecules-BIOBADADERM Registry., PMID:40387427
Ethanol-induced dysfunction of the mesenteric perivascular adipose tissue is driven by mineralocorticoid receptors., PMID:40377659
Cardiovascular Outcomes of Disease-Modifying Antirheumatic Drugs in Rheumatoid Arthritis: A Review of the Current Evidence., PMID:40376321
Efficacy of Tumor Necrosis Factor Inhibitors for Refractory Leg Ulcers in Cutaneous Polyarteritis Nodosa: A Case Series., PMID:40374513
Drug survival and predictor factors for discontinuation of first-line biologic therapy in rheumatoid arthritis: data from a real-world single-centre study., PMID:40371548
Drug-associated infections and infestations in older adults with tumor necrosis factor-alpha inhibitors: a real-world retrospective and pharmacovigilance study., PMID:40371340
Real-World Insights From Türkiye: Biologic DMARDs Usage in Spondyloarthritis Patients With Chronic Kidney Disease., PMID:40358366
Assessing real-world treatment with SDZ ETN (an etanercept biosimilar) in people with rheumatic diseases included in the COMPACT study: a plain language summary., PMID:40353738
Subclinical Enthesitis in Children with Chronic Nonbacterial Osteomyelitis., PMID:40353601
Aggregate Distributional Cost-Effectiveness Analysis of Biologics for the Treatment of Ankylosing Spondylitis in Chile., PMID:40329067
Compatibility between Proteins and Polysaccharide Excipients in Oral Delivery Tablets., PMID:40323221
TNF-α antagonist alleviates muscular layer enlargement but does not prevent myenteric neuronal loss in the colon of streptozotocin-induced diabetic rats., PMID:40288461
Impact of excess weight on clinical features of psoriasis and efficacy of biologic therapies in children with severe psoriasis: Analysis of data from the BiPe cohorts., PMID:40286464
Patient-Reported Outcome Measures in Patients with Rheumatoid Arthritis, Psoriasis, or Psoriatic Arthritis Treated with GP2015, an Etanercept Biosimilar: Results from Two Phase III Studies (EGALITY and EQUIRA)., PMID:40279006
The impact of biosimilar use on healthcare utilization among new users of etanercept for inflammatory arthritis: a population-based regression discontinuity analysis., PMID:40270586
Predicting extra-musculoskeletal and peripheral manifestations and their role on biologic treatment in patients with axial spondyloarthritis: TReasure experience., PMID:40264484
Recommendations for the use of DMARDs in pregnancy and reproductive health for patients with rheumatic disease: A scoping review., PMID:40256995
Divergent disruptive effects of soluble recombinant tau assemblies on synaptic plasticity in vivo., PMID:40251677
Characterising infusion/injection-related reactions in patients with rheumatoid arthritis treated with biologic agents., PMID:40249052
Relapsing-remitting multiple sclerosis as a potential consequence of thalidomide treatment: A case report., PMID:40245782
Stevens-Johnson Syndrome and Toxic Epidermal Necrolysis: A Review of Current Management and Innovative Therapies., PMID:40231717
Risk of Serious Infections in Patients Treated With Biologic or Targeted-synthetic Disease Modifying Antirheumatic Drugs in Qatar., PMID:40230031
Drug- and Vaccine-Induced Cutaneous T-Cell Lymphoma: A Systematic Review of the Literature., PMID:40226161
Immunotherapy as a treatment for type 1 diabetes mellitus in children and young adults: A comprehensive systematic review and meta-analysis., PMID:40215252
Effects of biological agents on lipid profile and hemogram parameters in patients with psoriasis., PMID:40208364
Dual biological treatments in immune-mediated disorders: a single center experience., PMID:40200173
From the Cochrane Library: Anti-tumor necrosis factor agents for pediatric psoriasis., PMID:40187532
Optimizing Maternal and Fetal Antibody Exposure and Dosing Regimens During Pregnancy Using a Physiologically Based Pharmacokinetic Model., PMID:40181734
Analysis of the Reduction in the Duration of Sick Leave for 32,512 Psoriasis Patients Following the Integration of Targeted Therapies for Psoriatic Disease into the Brazilian Healthcare System: a Retrospective Cohort Study., PMID:40171531
Anti-TNFα as an Adjunctive Therapy in Pancreas and Kidney Transplantation., PMID:40170787
Mechanism and Efficacy of Etanercept in Treating Autoimmune-like Manifestations of Coronavirus Disease 2019 in elderly individuals., PMID:40168796
The Efficacy, Safety and Longevity of Biologic Treatments in Pediatric and Adult Psoriasis Patients: A Comparative Multi-Center, Real-Life Study., PMID:40165569
For research use only. Not for human or drug use.
