Catalog No.
KAD12601
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Bevacizumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Bevacizumab will be captured by immobilized Bevacizumab. After washing away any unbound substances, a biotin-labeled Bevacizumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Bevacizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Bevacizumab concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
9.38 - 600 ng/mL
Sensitivity
5.78 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
290.2 |
71.3 |
15.2 |
294.6 |
72.8 |
15.5 |
|
Standard deviation |
8.9 |
2.4 |
1.3 |
6.8 |
2.3 |
1.7 |
|
CV (%) |
3.1 |
3.4 |
8.7 |
2.3 |
3.2 |
11.1 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
12-IgG1,F(ab)-12 IgG1,Fab-12 IgG1,rhuMAb-VEGF, ABP 215,CAS: 216974-75-3
For research use only. Not for human or drug use.
