Catalog No.
KAB94403
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Certolizumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Certolizumab will be captured by immobilized Certolizumab. After washing away any unbound substances, a biotin-labeled Certolizumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Certolizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Certolizumab concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
62.5 - 4,000 ng/mL
Sensitivity
8.11 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
1879.7 |
407.3 |
94.0 |
1841.5 |
319.0 |
70.6 |
|
Standard deviation |
82.6 |
18.9 |
9.3 |
99.4 |
15.8 |
6.9 |
|
CV (%) |
4.4 |
4.6 |
9.9 |
5.4 |
4.9 |
9.7 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
CDP870,PHA-738144, Certolizumab pegol,CAS: 428863-50-7
For research use only. Not for human or drug use.
