Catalog No.
KAD12602
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Ranibizumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Ranibizumab will be captured by immobilized Ranibizumab. After washing away any unbound substances, a biotin-labeled Ranibizumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Ranibizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Ranibizumab concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
62.5 - 4,000 ng/mL
Sensitivity
9.17 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
1980.3 |
509.3 |
119.3 |
2293.3 |
539.8 |
155.5 |
|
Standard deviation |
110.1 |
28.4 |
6.3 |
201.6 |
60.0 |
20.7 |
|
CV (%) |
5.6 |
5.6 |
5.3 |
8.8 |
11.1 |
13.3 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
Fab-12 variant Y0317,RhuFab,CAS: 347396-82-1
For research use only. Not for human or drug use.
