Anti-Obinutuzumab ELISA Kit

Catalog No.

KAC90702

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Obinutuzumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Obinutuzumab will be captured by immobilized Obinutuzumab. After washing away any unbound substances, a biotin-labeled Obinutuzumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Obinutuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Obinutuzumab concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

6.25 - 400 ng/mL

Sensitivity

2.29 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	194.0	46.4	11.3	191.2	47.0	9.8
Standard deviation	8.1	2.2	1.1	7.7	4.1	1.2
CV (%)	4.2	4.7	10.0	4.0	8.7	12.0

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

GA101,RG7159,RO5072759,Afutuzumab,CAS: 949142-50-1

Document Download

Anti-Obinutuzumab ELISA Kit.pdf