Anti-Sarilumab ELISA Kit

Catalog No.

KAC36902

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Sarilumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Sarilumab will be captured by immobilized Sarilumab. After washing away any unbound substances, a biotin-labeled Sarilumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Sarilumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Applications

Used for the quantitative determination of Anti-Sarilumab concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

0.78 -50 ng/mL

Sensitivity

0.46 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	26.3	5.9	1.2	22.4	5.7	1.3
Standard deviation	1.3	0.6	0.1	1.4	0.4	0.2
CV (%)	4.8	9.9	9.9	6.3	6.6	14.5

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.

Alternative Names

REGN88,SAR153191, CAS: 1189541-98-7

Document Download

Anti-Sarilumab ELISA Kit.pdf