Catalog No.
KAH02202
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Pembrolizumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Pembrolizumab will be captured by immobilized Pembrolizumab. After washing away any unbound substances, a biotin-labeled Pembrolizumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Pembrolizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Pembrolizumab concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
3.13 - 200 ng/mL
Sensitivity
1.96 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
94.6 |
24.4 |
5.9 |
97.5 |
23.1 |
5.1 |
|
Standard deviation |
3.5 |
1.0 |
0.5 |
4.1 |
1.7 |
0.5 |
|
CV (%) |
3.7 |
4.0 |
9.0 |
4.2 |
7.2 |
9.8 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
MK-3475, CAS: 1374853-91-4
For research use only. Not for human or drug use.
