Catalog No.
PHG32401
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human TP53BP1 (Ala1614-His1972).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:1000-1:4000
Target
TP53-binding protein 1,TP53BP1,p53-binding protein 1,p53BP1,53BP1
Concentration
3.59 mg/ml
Purification
Purified by antigen affinity column.
Accession
Q12888
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Selective USP7 Inhibition Synergizes with MEK1/2 Inhibitor to Enhance Immune Responses and Potentiate Anti-PD-1 Therapy in NRAS-Mutant Melanoma., PMID:40204067
Analysis for type of 53BP1 nuclear expression by immunofluorescence as an indicator of genomic instability in oropharyngeal squamous epithelial lesions., PMID:39528680
SARS-CoV-2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro., PMID:34696485
The impact of TP53BP1 and MLH1 on metastatic capability in cases of locally advanced prostate cancer and their usefulness in clinical practice., PMID:32280038
Assessment of DNA damage by 53PB1 and pKu70 detection in peripheral blood lymphocytes by immunofluorescence and high-resolution transmission electron microscopy., PMID:32006067
Biodosimetry of Low Dose Ionizing Radiation Using DNA Repair Foci in Human Lymphocytes., PMID:31947954
Immunofluorescence analysis of DNA damage response protein p53-binding protein 1 in a case of uterine dedifferentiated leiomyosarcoma arising from leiomyoma., PMID:31570279
53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function., PMID:31390554
A Novel Diagnostic Method for Thyroid Follicular Tumors Based on Immunofluorescence Analysis of p53-Binding Protein 1 Expression: Detection of Genomic Instability., PMID:30929573
Expression pattern of p53-binding protein 1 as a new molecular indicator of genomic instability in bladder urothelial carcinoma., PMID:30341375
Differential responses of MET activations to MET kinase inhibitor and neutralizing antibody., PMID:30208970
SHLD2/FAM35A co-operates with REV7 to coordinate DNA double-strand break repair pathway choice., PMID:30154076
DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity., PMID:29656893
Particles with similar LET values generate DNA breaks of different complexity and reparability: a high-resolution microscopy analysis of γH2AX/53BP1 foci., PMID:29271466
Prostate cancer treated with brachytherapy; an exploratory study of dose-dependent biomarkers and quality of life., PMID:28288658
Regulation of pairing between broken DNA-containing chromatin regions by Ku80, DNA-PKcs, ATM, and 53BP1., PMID:28155885
Chromatin organization revealed by nanostructure of irradiation induced γH2AX, 53BP1 and Rad51 foci., PMID:28094292
Localized Movement and Levels of 53BP1 Protein Are Changed by γ-irradiation in PML Deficient Cells., PMID:27526954
Deficiency of 53BP1 inhibits the radiosensitivity of colorectal cancer., PMID:27499037
Vitamin D receptor signaling improves Hutchinson-Gilford progeria syndrome cellular phenotypes., PMID:27145372
Pneumococcal Pneumolysin Induces DNA Damage and Cell Cycle Arrest., PMID:27026501
Analysis of Lymphocytic DNA Damage in Early Multiple Sclerosis by Automated Gamma-H2AX and 53BP1 Foci Detection: A Case Control Study., PMID:26820970
ATM Localization and Heterochromatin Repair Depend on Direct Interaction of the 53BP1-BRCT2 Domain with γH2AX., PMID:26628370
Unirradiated cells rescue cells exposed to ionizing radiation: Activation of NF-κB pathway in irradiated cells., PMID:26524645
53BP1 suppresses epithelial-mesenchymal transition by downregulating ZEB1 through microRNA-200b/429 in breast cancer., PMID:26011542
Calibration of the γ-H2AX DNA double strand break focus assay for internal radiation exposure of blood lymphocytes., PMID:25853575
Ubiquitin-H2AX fusions render 53BP1 recruitment to DNA damage sites independent of RNF8 or RNF168., PMID:25695757
53BP1 promotes microhomology-mediated end-joining in G1-phase cells., PMID:25586219
Inhibition of EGFR or IGF-1R signaling enhances radiation response in head and neck cancer models but concurrent inhibition has no added benefit., PMID:25355701
EGFR-inhibition enhances apoptosis in irradiated human head and neck xenograft tumors independent of effects on DNA repair., PMID:24059677
Co-operation of BRCA1 and POH1 relieves the barriers posed by 53BP1 and RAP80 to resection., PMID:24013561
Smad7 foci are present in micronuclei induced by heavy particle radiation., PMID:23643526
Radiosensitivity in breast cancer assessed by the histone γ-H2AX and 53BP1 foci., PMID:23617930
53BP1 expression is a modifier of the prognostic value of lymph node ratio and CA 19-9 in pancreatic adenocarcinoma., PMID:23530749
Alteration of the DNA damage response in colorectal tumor progression., PMID:23332927
The histone methyltransferase MMSET regulates class switch recombination., PMID:23241889
Three-dimensional imaging reveals the spatial separation of γH2AX-MDC1-53BP1 and RNF8-RNF168-BRCA1-A complexes at ionizing radiation-induced foci., PMID:22633816
DNA damage foci formation and decline in two-dimensional monolayers and in three-dimensional human vessel models: differential effects according to radiation quality., PMID:22449005
Significance of p53-binding protein 1 nuclear foci in uterine cervical lesions: endogenous DNA double strand breaks and genomic instability during carcinogenesis., PMID:22034884
Regulation of the BRCA1 gene by an SRC3/53BP1 complex., PMID:21914189
Recruitment of proteins to DNA double-strand breaks: MDC1 directly recruits RAP80., PMID:21857162
Human genetic variants of homologous recombination repair genes first found to be associated with Epstein-Barr virus antibody titers in healthy Cantonese., PMID:21792882
ATM-dependent phosphorylation of 53BP1 in response to genomic stress in oxic and hypoxic cells., PMID:21680038
In vivo formation of gamma-H2AX and 53BP1 DNA repair foci in blood cells after radioiodine therapy of differentiated thyroid cancer., PMID:20660387
Sensitivity to poly(ADP-ribose) polymerase (PARP) inhibition identifies ubiquitin-specific peptidase 11 (USP11) as a regulator of DNA double-strand break repair., PMID:20233726
DNA repair alterations in children with pediatric malignancies: novel opportunities to identify patients at risk for high-grade toxicities., PMID:20153123
Androgen receptor interacts with telomeric proteins in prostate cancer cells., PMID:20110352
53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair., PMID:20081839
The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination., PMID:20080757
Dose response and kinetics of foci disappearance following exposure to high- and low-LET ionizing radiation., PMID:19863201
For research use only. Not for human or drug use.
