Anti-Mad3 Antibody (N129A/6)

Catalog No.

RHN17102

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

Class C basic helix-loop-helix protein 13, Max-associated protein 3, Max dimerizer 3, bHLHc13, BHLHC13, Myx, Max dimerization protein 3, MXD3, Max-interacting transcriptional repressor MAD3, MAD3

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

N129A/6

Cripto-1 acts as a molecular bridge linking nodal to ALK4 via distinct structural domains., PMID:39840816

Analysis of the DNA-binding properties of TGF-β-activated Smad complexes unveils a possible molecular basis for cellular context-dependent signaling., PMID:39114961

TGF-β1 induces PD-1 expression in macrophages through SMAD3/STAT3 cooperative signaling in chronic inflammation., PMID:38441961

Investigating dysregulation of TGF-β1/SMAD3 signaling in atopic dermatitis: a molecular and immunohistochemical analysis., PMID:38066678

Analysis of intronic SNP (rs4147358) and expression of SMAD3 gene in Atopic Dermatitis: A case-control study., PMID:37100019

Identification immune response genes in psoriasis after treatment with secukinumab., PMID:37029373

EGF Contributes to Hypertrophy of Human Ligamentum Flavum via the TGF-β1/Smad3 Signaling Pathway., PMID:36185336

Mismatch repair-deficient prostate cancer with parenchymal brain metastases treated with immune checkpoint blockade., PMID:34140335

A system-view of Bordetella pertussis booster vaccine responses in adults primed with whole-cell versus acellular vaccine in infancy., PMID:33690224

Refractory systemic juvenile idiopathic arthritis successfully treated with rapamycin., PMID:33547778

Luspatercept in the treatment of lower-risk myelodysplastic syndromes., PMID:33511859

Micellar Casein and Whey Powder Hold a TGF-β Activity and Regulate ID Genes In Vitro., PMID:33477984

Targeting TGF-β-Mediated SMAD Signaling Pathway via Novel Recombinant Cytotoxin II: A Potent Protein from Naja naja oxiana Venom in Melanoma., PMID:33167431

Cyclin E overexpression confers resistance to trastuzumab through noncanonical phosphorylation of SMAD3 in HER2+ breast cancer., PMID:33054513

Full-length IL-33 regulates Smad3 phosphorylation and gene transcription in a distinctive AP2-dependent manner., PMID:32977155

Atorvastatin attenuates TGF‑β1‑induced fibrogenesis by inhibiting Smad3 and MAPK signaling in human ventricular fibroblasts., PMID:32468059

Effect of CD44 on differentiation of human amniotic mesenchymal stem cells into chondrocytes via Smad and ERK signaling pathways., PMID:32236637

TGF-β pathway activation by idiopathic pulmonary fibrosis (IPF) fibroblast derived soluble factors is mediated by IL-6 trans-signaling., PMID:32070329

Biological basis for efficacy of activin receptor ligand traps in myelodysplastic syndromes., PMID:31961337

A novel NFKBIA variant substituting serine 36 of IκBα causes immunodeficiency with warts, bronchiectasis and juvenile rheumatoid arthritis in the absence of ectodermal dysplasia., PMID:31683054

Integrin αvβ6 mediates epithelial-mesenchymal transition in human bronchial epithelial cells induced by lipopolysaccharides of Pseudomonas aeruginosa via TGF-β1-Smad2/3 signaling pathway., PMID:31243731

Blockade of thrombospondin-1 ameliorates high glucose-induced peritoneal fibrosis through downregulation of TGF-β1/Smad3 signaling pathway., PMID:31236971

Indoxyl Sulfate-Induced Extracellular Vesicles Released from Endothelial Cells Stimulate Vascular Smooth Muscle Cell Proliferation by Inducing Transforming Growth Factor-Beta Production., PMID:31085925

A non-autonomous role of MKL1 in the activation of hepatic stellate cells., PMID:30951901

α-PD-1 therapy elevates Treg/Th balance and increases tumor cell pSmad3 that are both targeted by α-TGFβ antibody to promote durable rejection and immunity in squamous cell carcinomas., PMID:30832732

Tumour necrosis factor-α-induced protein 8-like 2 is a novel regulator of proliferation, migration, and invasion in human rectal adenocarcinoma cells., PMID:30637920

Inhibitory Antibodies against Activin A and TGF-β Reduce Self-Supported, but Not Soluble Factors-Induced Growth of Human Pulmonary Arterial Vascular Smooth Muscle Cells in Pulmonary Arterial Hypertension., PMID:30274147

Alterations of MEK1/2-ERK1/2, IFNγ and Smad2/3 associated Signalling pathways during cryopreservation of ASCs affect their differentiation towards VSMC-like cells., PMID:30273905

EWSR1-SMAD3-rearranged Fibroblastic Tumor: An Emerging Entity in an Increasingly More Complex Group of Fibroblastic/Myofibroblastic Neoplasms., PMID:29957732

Platelet-Derived TGF-β Induces Tissue Factor Expression via the Smad3 Pathway in Osteosarcoma Cells., PMID:29949655

Significance of pSmad2/3 and Smad4 in hepatitis C virus-related liver fibrosis and hepatocellular carcinoma., PMID:29924446

Phenytoin activates Smad3 phosphorylation and periostin expression in drug-induced gingival enlargement., PMID:29916554

Astragalus polysaccharides exerts anti-infective activity by inducing human cathelicidin antimicrobial peptide LL-37 in respiratory epithelial cells., PMID:29672953

Itraconazole inhibits invasion and migration of pancreatic cancer cells by suppressing TGF-β/SMAD2/3 signaling., PMID:29484419

Blockade of KCa3.1: A novel target to treat TGF-β1 induced conjunctival fibrosis., PMID:29242028

Expression pattern and phosphorylation status of Smad2/3 in different subtypes of human first trimester trophoblast., PMID:28864007

Amniotic membrane stimulates cell migration by modulating transforming growth factor-β signalling., PMID:28621502

DcR3 promotes hepatoma cell migration by downregulating E-cadherin expression., PMID:28560426

Functional importance of stripping in NFκB signaling revealed by a stripping-impaired IκBα mutant., PMID:28167786

HLA-Cw6 allele, NFkB1 and NFkBIA polymorphisms play no role in predicting response to etanercept in psoriatic patients., PMID:27348478

Anti-inflammatory effect of curcumin on mast cell-mediated allergic responses in ovalbumin-induced allergic rhinitis mouse., PMID:26507910

Lactobacillus acidophilus attenuates Salmonella-induced intestinal inflammation via TGF-β signaling., PMID:26446848

Epigenetic regulation of Smad2 and Smad3 by profilin-2 promotes lung cancer growth and metastasis., PMID:26354229

Intrinsic Differences in Donor CD4 T Cell IL-2 Production Influence Severity of Parent-into-F1 Murine Lupus by Skewing the Immune Response Either toward Help for B Cells and a Sustained Autoantibody Response or toward Help for CD8 T Cells and a Downregulatory Th1 Response., PMID:26320249

Critical role of phosphorylation of serine 165 of YBX1 on the activation of NF-κB in colon cancer., PMID:26318844

CHMP5 controls bone turnover rates by dampening NF-κB activity in osteoclasts., PMID:26195726

Regulation of complement-dependent cytotoxicity by TGF-β-induced epithelial-mesenchymal transition., PMID:26148233

Expansion and long-term culture of human spermatogonial stem cells via the activation of SMAD3 and AKT pathways., PMID:26088866

Nerve growth factor exposure promotes tubular epithelial-mesenchymal transition via TGF-β1 signaling activation., PMID:26066770

Inhibitory Effect of Tranilast on Transforming Growth Factor-Beta-Induced Protein in Granular Corneal Dystrophy Type 2 Corneal Fibroblasts., PMID:26020822

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