Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Polyclonal Antibody

Anti-PPIA Polyclonal Antibody

Catalog #:   PHF55601 Specific References (24) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P62937
Overview

Catalog No.

PHF55601

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPIA (Val2-Glu165).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PPIase A,Cyclosporin A-binding protein,Peptidyl-prolyl cis-trans isomerase A,Cyclophilin A,PPIA,CYPA,Rotamase A

Purification

Purified by antigen affinity column.

Accession

P62937

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with PPIA antibody (PHF55601) at 0.4μg/ml.

    Lane 1: Raji cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: NIH/3T3 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
  • Immunocytochemistry/ Immunofluorescence
    PPIA in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with PPIA (PHF55601) at 8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PPIA antibody (PHF55601) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
References

Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients., PMID:36122652

Comparative systeomics to elucidate physiological differences between CHO and SP2/0 cell lines., PMID:35228567

Inhibition of Cathepsin B and SAPC Secreted by HIV-Infected Macrophages Reverses Common and Unique Apoptosis Pathways., PMID:34994563

Identification of resistance pathways and therapeutic targets in relapsed multiple myeloma patients through single-cell sequencing., PMID:33619369

Serum Analysis of Women with Early-Stage Breast Cancer Using a Mini-Array of Tumor-Associated Antigens., PMID:33096879

Construction of Genomic Library and High-Throughput Screening of Pseudomonas aeruginosa Novel Antigens for Potential Vaccines., PMID:32779581

Influence of elevated protein and tannin-rich peanut skin supplementation on growth performance, blood metabolites, carcass traits and immune-related gene expression of grazing meat goats., PMID:31724236

Ole e 15 and its human counterpart -PPIA- chimeras reveal an heterogeneous IgE response in olive pollen allergic patients., PMID:31636292

Determination of microcystin-LR, employing aptasensors., PMID:30121422

Mycobacterium tuberculosis Peptidyl-Prolyl Isomerases Are Immunogenic, Alter Cytokine Profile and Aid in Intracellular Survival., PMID:28261567

Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody., PMID:26865113

A multiparametric serum marker panel as a complementary test to mammography for the diagnosis of node-negative early-stage breast cancer and DCIS in young women., PMID:24957886

Magnetic particle-based enzyme assays and immunoassays for microcystins: from colorimetric to electrochemical detection., PMID:23214443

Surface lipoprotein PpiA of Streptococcus mutans suppresses scavenger receptor MARCO-dependent phagocytosis by macrophages., PMID:21986627

Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer., PMID:21165559

[Modified serum-guided immunoblotting for differential proteomic study of prostate cancer]., PMID:20684326

Insulin-degrading enzyme binds to the nonglycosylated precursor of varicella-zoster virus gE protein found in the endoplasmic reticulum., PMID:19864391

Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women., PMID:19584157

Enzymatic recycling-based amperometric immunosensor for the ultrasensitive detection of okadaic acid in shellfish., PMID:18775658

[Application of ELISA for microcystins detection]., PMID:17712969

Evidence that phosphorylation of the RNA polymerase II carboxyl-terminal repeats is similar in yeast and humans., PMID:16012166

ABO-incompatible living-donor kidney transplantation in children., PMID:11579297

The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines., PMID:10748200

Phenotype versus genotype of the 19 kD peptido-glycan associated protein of Legionella (PpIA), among Legionellae and other gram-negative bacteria., PMID:1816489

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-PPIA Polyclonal Antibody [PHF55601]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2025 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only