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Anti-EV71 VP1/Capsid protein VP1 Polyclonal Antibody

Catalog #:   PVV20301 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: A0A894JU04
Overview

Catalog No.

PVV20301

Species reactivity

Human enterovirus 71 (EV71) (EV-71)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant EV71 VP1/Capsid protein VP1 (Gly566-Leu862).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:200, WB: 1:500-1:4000

Target

Genome polyprotein, P1, Capsid protein VP1, P1D, Virion protein 1

Concentration

0.5 mg/ml

Purification

Purified by antigen affinity column.

Accession

A0A894JU04

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with VP1/Capsid protein VP1 antibody (PVV20301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 34 kDa
    Observed MW: 34 kDa
References

Immunogenicity and immunodominant linear B-cell epitopes of a new DNA-based tetravalent vaccine against four major enteroviruses causing hand, foot, and mouth disease., PMID:38705805

Variant enterovirus A71 found in immune-suppressed patient binds to heparan sulfate and exhibits neurotropism in B-cell-depleted mice., PMID:37058406

Intranasal immunization with curdlan induce Th17 responses and enhance protection against enterovirus 71., PMID:36863926

[Preparation of monoclonal antibody against enterovirus 71 capsid protein VP1]., PMID:35365993

Does enterovirus 71 urge for effective vaccine control strategies? Challenges and current opinion., PMID:34997684

[Preparation of pan-neutralizing mouse monoclonal antibodies against enterovirus 71 and coxsackievirus A16]., PMID:34140076

Chimeric enterovirus 71 virus-like particle displaying conserved coxsackievirus A16 epitopes elicits potent immune responses and protects mice against lethal EV71 and CA16 infection., PMID:34116877

Transplantation of Enterovirus 71 Virion Protein Particle Vaccine Protects Against Enterovirus 71 Infection in a Neonatal Mouse Model., PMID:33397838

Recombinant Enterovirus 71 Viral Protein 1 Fused to a Truncated Newcastle Disease Virus NP (NPt) Carrier Protein., PMID:33297428

Human Antibodies to VP4 Inhibit Replication of Enteroviruses Across Subgenotypes and Serotypes, and Enhance Host Innate Immunity., PMID:33101238

Epitope Peptide Amphiphile-Based Nanofiber as an Effective Vaccine for Viral Infectious Diseases., PMID:32331099

Plant-Produced Anti-Enterovirus 71 (EV71) Monoclonal Antibody Efficiently Protects Mice Against EV71 Infection., PMID:31805650

VP1 of Enterovirus 71 Protects Mice Against Enterovirus 71 and Coxsackievirus B3 in Lethal Challenge Experiment., PMID:31787970

Target-Induced Cascade Amplification for Homogeneous Virus Detection., PMID:31698906

Development and characterization of an enterovirus 71 (EV71) virus-like particles (VLPs) vaccine produced in Pichia pastoris., PMID:31403352

Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection., PMID:31285854

Enterovirus type 71-immunized chicken egg yolk immunoglobulin has cross antiviral activity against coxsackievirus A16 in vitro., PMID:31258670

Recombinant lactococcus lactis secreting viral protein 1 of enterovirus 71 and its immunogenicity in mice., PMID:31119429

A potential therapeutic neutralization monoclonal antibody specifically against multi-coxsackievirus A16 strains challenge., PMID:30735461

The mature EV71 virion induced a broadly cross-neutralizing VP1 antibody against subtypes of the EV71 virus., PMID:30650163

Induction of immune responses by a novel recombinant fusion protein of enterovirus A71 in BALB/c mice., PMID:30465931

Analysis of an Imported Subgenotype C2 Strain of Human Enterovirus 71 in Beijing, China, 2015., PMID:30323801

[Preparation of mouse monoclonal antibodies against EV71 capsid protein VP1]., PMID:30043737

VP1 Amino Acid Residue 145 of Enterovirus 71 Is a Key Residue for Its Receptor Attachment and Resistance to Neutralizing Antibody during Cynomolgus Monkey Infection., PMID:29848582

Non-neutralizing Antibody Responses against VP1 in Enterovirus A, B, C and Rhinovirus A species among Infants and Children in Shanghai., PMID:29615683

Induction of a high-titered antibody response using HIV gag-EV71 VP1-based virus-like particles with the capacity to protect newborn mice challenged with a lethal dose of enterovirus 71., PMID:29582164

Identification and production of mouse scFv to specific epitope of enterovirus-71 virion protein-2 (VP2)., PMID:29356992

Poorly neutralizing polyclonal antibody in vitro against coxsackievirus A16 circulating strains can prevent a lethal challenge in vivo., PMID:29337652

Hepatitis B virus core particles containing multiple epitopes confer protection against enterovirus 71 and coxsackievirus A16 infection in mice., PMID:29129453

Alanine scanning mutagenesis of SP70 epitope in characterizing species‑specific antibodies induced by enterovirus 71‑based antigens., PMID:29115505

Specific Unbinding Forces Between Mutated Human P-Selectin Glycoprotein Ligand-1 and Viral Protein-1 Measured Using Force Spectroscopy., PMID:29016136

A novel combined vaccine based on monochimeric VLP co-displaying multiple conserved epitopes against enterovirus 71 and varicella-zoster virus., PMID:28408118

Recombinant heat shock protein 78 enhances enterovirus 71 propagation in Vero cells and is induced in SK-N-SH cells during the infection., PMID:28236071

A Molecular Approach Applied to Enteroviruses Surveillance in Northern Taiwan, 2008-2012., PMID:27907198

A novel inactivated enterovirus 71 vaccine can elicit cross-protective immunity against coxsackievirus A16 in mice., PMID:27771182

Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1., PMID:27618897

[Preparation of monoclonal antibodies against enterovirus type 71 with an epitope-incorporated adenovirus type 3 vector]., PMID:27412945

Vesicular stomatitis virus-based vaccines expressing EV71 virus-like particles elicit strong immune responses and protect newborn mice from lethal challenges., PMID:27373596

Enhancing immune responses of EV71 VP1 DNA vaccine by co-inoculating plasmid IL-12 or GM-CSF expressing vector in mice., PMID:27188732

Establishment of an animal challenge model as a potency assay for an inactivated Enterovirus Type 71 vaccine., PMID:27068365

Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody., PMID:26938634

Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals., PMID:26917423

Enterovirus 71 transmission by exosomes establishes a productive infection in human neuroblastoma cells., PMID:26837894

Protein microarray-mediated detection of antienterovirus antibodies in serum., PMID:26831405

EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge., PMID:26809443

Pros and cons of VP1-specific maternal IgG for the protection of Enterovirus 71 infection., PMID:26529069

Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry., PMID:26401034

Development of a Specific Latex Agglutination Test to Detect Antibodies of Enterovirus 71., PMID:26363276

Hexon-modified recombinant E1-deleted adenoviral vectors as bivalent vaccine carriers for Coxsackievirus A16 and Enterovirus 71., PMID:26296491

A Broadly Cross-protective Vaccine Presenting the Neighboring Epitopes within the VP1 GH Loop and VP2 EF Loop of Enterovirus 71., PMID:26243660

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-EV71 VP1/Capsid protein VP1 Polyclonal Antibody [PVV20301]
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