Catalog No.
PHF46901
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SUMO2 (Met1-Tyr95).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Ubiquitin-like protein SMT3B,SMT3B,SUMO-3,Smt3B,Sentrin-2,SMT3 homolog 2,SMT3H2,SUMO2,HSMT3,Small ubiquitin-related modifier 2,SUMO-2
Purification
Purified by antigen affinity column.
Accession
P61956
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
A highly sensitive nanochannel device for the detection of SUMO1 peptides., PMID:37564410
An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway., PMID:36860866
SUMO monoclonal antibodies vary in sensitivity, specificity, and ability to detect types of SUMO conjugate., PMID:36494414
Proteomics datasets of developing rat brain: Synaptic proteome and SUMO2/3-ylome., PMID:35516005
Exploration of autoantibody responses in canine diabetes using protein arrays., PMID:35169238
Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain., PMID:34887727
SUMO-Modification of Human Nrf2 at K110 and K533 Regulates Its Nucleocytoplasmic Localization, Stability and Transcriptional Activity., PMID:33770425
Antibody-free enrichment method for proteome-wide analysis of endogenous SUMOylation sites., PMID:33736815
Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation., PMID:33472076
SUMOylation Is Required for ERK5 Nuclear Translocation and ERK5-Mediated Cancer Cell Proliferation., PMID:32209980
Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology., PMID:29138295
SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells., PMID:29038616
Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins., PMID:27631801
Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions Between SUMO Traps and Mono- or PolySUMO Moieties., PMID:27631800
IL-6 induced proliferation and cytotoxic activity of CD8(+) T cells is elevated by SUMO2 overexpression., PMID:27071615
Lung neuroendocrine tumors: correlation of ubiquitinylation and sumoylation with nucleo-cytosolic partitioning of PTEN., PMID:25884169
The SUMO-targeted ubiquitin ligase RNF4 localizes to etoposide-exposed mitotic chromosomes: implication for a novel DNA damage response during mitosis., PMID:24695317
Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies., PMID:24651501
In vivo characterization of the properties of SUMO1-specific monobodies., PMID:24040933
Detecting endogenous SUMO targets in mammalian cells and tissues., PMID:23503365
SUMOylation of the inducible (c-Fos:c-Jun)/AP-1 transcription complex occurs on target promoters to limit transcriptional activation., PMID:23396363
Identification of endogenous SUMO1 accepter sites by mass spectrometry., PMID:22665316
Ubiquitin-related proteins in neuronal and glial intranuclear inclusions in intranuclear inclusion body disease., PMID:22612509
Small ubiquitin-like modifier (SUMO) modification mediates function of the inhibitory domains of developmental regulators FOXC1 and FOXC2., PMID:22493429
Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation., PMID:22118216
Complex SUMO-1 regulation of cardiac transcription factor Nkx2-5., PMID:21931855
Evidence Implicating CCNB1IP1, a RING Domain-Containing Protein Required for Meiotic Crossing Over in Mice, as an E3 SUMO Ligase., PMID:21779533
SUMO proteins are involved in the stress response during spermatogenesis and are localized to DNA double-strand breaks in germ cells., PMID:20385780
Nuclear proteome analysis of monkey embryonic stem cells during differentiation., PMID:20091144
"ChopNSpice," a mass spectrometric approach that allows identification of endogenous small ubiquitin-like modifier-conjugated peptides., PMID:19721078
[Prokaryotic expression, purification and preparation of polyclonal antibody for human SUMO-2 gene]., PMID:18616917
Stabilization of PML nuclear localization by conjugation and oligomerization of SUMO-3., PMID:15940266
Small ubiquitin-related modifiers: A novel and independent class of autoantigens in primary biliary cirrhosis., PMID:15726652
Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1., PMID:12665592
A novel mammalian Smt3-specific isopeptidase 1 (SMT3IP1) localized in the nucleolus at interphase., PMID:11029585
SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage., PMID:10759568
Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3., PMID:10692421