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Anti-B2M Polyclonal Antibody

Catalog #:   PHF46101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P61769
Overview

Catalog No.

PHF46101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human B2M (Gln22-Met119).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Beta-2-microglobulin,B2M

Purification

Purified by antigen affinity column.

Accession

P61769

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with B2M antibody (PHF46101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 14 kDa
    Observed MW: 14 kDa
  • Immunocytochemistry/ Immunofluorescence
    B2M in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with B2M (PHF46101) at 7.4 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Intratumoral IL12 mRNA administration activates innate and adaptive pathways in checkpoint inhibitor resistant tumors resulting in complete responses., PMID:40321762

An Exosomal mRNA Urine Test for Detection and Risk Stratification of Human Kidney Transplant Rejection., PMID:40303229

Interim PET after 4 cycles predicts outcome in histomolecularly confirmed primary mediastinal B-cell lymphoma., PMID:40030008

Clinico-genomic features predict distinct metastatic phenotypes in cutaneous melanoma., PMID:39975034

A self-activated and protective module enhances the preclinical performance of allogeneic anti-CD70 CAR-T cells., PMID:39906740

Craters on the melanoma surface facilitate tumor-immune interactions and demonstrate pathologic response to checkpoint blockade in humans., PMID:39345527

Conformational Alterations of the Cell Surface of Monomeric and Dimeric β2m-Free HLA-I (Proto-HLA) May Enable Novel Immune Functions in Health and Disease., PMID:39057057

Innate and adaptive immune response of Rainbow trout (Oncorhynchus mykiss) naturally infected with Yersinia ruckeri., PMID:38960109

IFNγ mediates the resistance of tumor cells to distinct NK cell subsets., PMID:38955423

BCMA/CD47-directed universal CAR-T cells exhibit excellent antitumor activity in multiple myeloma., PMID:38783333

Prostate cancer research: tools, cell types, and molecular targets., PMID:38595814

Long-lasting mRNA-encoded interleukin-2 restores CD8+ T cell neoantigen immunity in MHC class I-deficient cancers., PMID:38490213

Differentially Expressed Genes Involved in Primary Resistance to Immunotherapy in Patients with Advanced-Stage Pulmonary Cancer., PMID:38396726

Diversity in the HLA-I Recognition of HLA-F Monoclonal Antibodies: HLA-F or HLA-Ib Monospecific, HLA-E or HLA-G Bispecific Antibodies with or without HLA-Ia Reactivity., PMID:38390869

Mechanism exploration and biomarker identification of glycemic deterioration in patients with diseases of the exocrine pancreas., PMID:38388766

Allogeneic CAR-T cells with of HLA-A/B and TRAC disruption exhibit promising antitumor capacity against B cell malignancies., PMID:38231412

Generation and optimization of off-the-shelf immunotherapeutics targeting TCR-Vβ2+ T cell malignancy., PMID:38225288

A targeted antibody-based array reveals a serum protein signature as biomarker for adolescent idiopathic scoliosis patients., PMID:37667193

Concurrent predictors of an immune responsive tumor microenvironment within tumor mutational burden-high breast cancer., PMID:37637072

Cell Surface B2m-Free Human Leukocyte Antigen (HLA) Monomers and Dimers: Are They Neo-HLA Class and Proto-HLA?, PMID:37627243

How I Manage Chronic Lymphocytic Leukemia., PMID:37606492

Immune Marker Spatial Distribution and Clinical Outcome after PD-1 Blockade in Mismatch Repair-deficient, Advanced Colorectal Carcinomas., PMID:37566222

Conversion of senescent cartilage into a pro-chondrogenic microenvironment with antibody-functionalized copper sulfate nanoparticles for efficient osteoarthritis therapy., PMID:37550685

Efficacy and safety of PD-1 monoclonal antibody plus rituximab in relapsed/refractory diffuse large B cell lymphoma patients., PMID:37294015

β2-Microglobulin coaggregates with Aβ and contributes to amyloid pathology and cognitive deficits in Alzheimer's disease model mice., PMID:37264159

Primary Mediastinal B-Cell Lymphoma in Children and Young Adults., PMID:36898366

β2-microglobulin functions as an endogenous NMDAR antagonist to impair synaptic function., PMID:36868208

Allele mining in gene encoding fc fragment of IgG receptor, transporter, alpha (FCGRT) and association of nucleotide variants with passive transfer of immunity in neonatal buffalo calves., PMID:36757548

A Comprehensive Biomarker Analysis of Microsatellite Unstable/Mismatch Repair Deficient Colorectal Cancer Cohort Treated with Immunotherapy., PMID:36613564

Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression., PMID:36593395

Risk Factors for and Outcomes of Follicular Lymphoma Histological Transformation at First Progression in the GALLIUM Study., PMID:36379880

Isotope-dilution liquid chromatography-tandem mass spectrometry method for serum beta 2-microglobulin quantification., PMID:36228451

Identification of immune cell infiltration and diagnostic biomarkers in unstable atherosclerotic plaques by integrated bioinformatics analysis and machine learning., PMID:36211422

[Enhanced Natural Killers with CISH and B2M Gene Knockouts Reveal Increased Cytotoxicity in Glioblastoma Primary Cultures]., PMID:36165021

Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients., PMID:36122652

A novel immune-related epigenetic signature based on the transcriptome for predicting the prognosis and therapeutic response of patients with diffuse large B-cell lymphoma., PMID:36055572

Donor natural killer cells trigger production of β-2-microglobulin to enhance post-bone marrow transplant immunity., PMID:35984965

Long follow-up of symptomatic Multiple Myeloma patients after Covid-19 vaccination (BNT162b2): A single-institution retrospective experience., PMID:35966626

β2-Microglobulin Maintains Glioblastoma Stem Cells and Induces M2-like Polarization of Tumor-Associated Macrophages., PMID:35841593

Chitosan oligosaccharide improves the mucosal immunity of small intestine through activating SIgA production in mice: Proteomic analysis., PMID:35569308

Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRV., PMID:35343384

Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors., PMID:35241815

CRISPR/Cas9-Engineered HLA-Deleted Glomerular Endothelial Cells as a Tool to Predict Pathogenic Non-HLA Antibodies in Kidney Transplant Recipients., PMID:35167486

Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis)., PMID:35152368

Partial immunoparesis contributes to risk of early infections in patients with multiple myeloma., PMID:35116375

Human IgE does not bind to human FcRn., PMID:34996950

Inhibition of FGFR Reactivates IFNγ Signaling in Tumor Cells to Enhance the Combined Antitumor Activity of Lenvatinib with Anti-PD-1 Antibodies., PMID:34753772

Pathological review of cardiac amyloidosis using autopsy cases in a single Japanese institution., PMID:34653913

Targeted clearance of senescent cells using an antibody-drug conjugate against a specific membrane marker., PMID:34645909

Pan-cancer analysis of longitudinal metastatic tumors reveals genomic alterations and immune landscape dynamics associated with pembrolizumab sensitivity., PMID:34446728

Datasheet

Document Download

Anti-B2M Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-B2M Polyclonal Antibody [PHF46101]
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