Catalog No.
KAD62651
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Dupilumab has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled Human CD124 and then pipetted into the wells. Anti-Dupilumab Neutralizing Antibody in the sample competitively binds to Dupilumab with the biotin-labeled Human CD124. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-Dupilumab Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-Dupilumab Neutralizing Antibody concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
46.88 - 3,000 ng/mL
Sensitivity
29.02 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
2112.5 |
445.2 |
97.2 |
2653.5 |
475.4 |
96.6 |
|
Standard deviation |
259.1 |
34.9 |
11.1 |
497.1 |
57.4 |
18.0 |
|
CV (%) |
12.3 |
7.8 |
11.4 |
18.7 |
12.1 |
18.6 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
CD124,REGN668,SAR231893, CAS: 1190264-60-8
For research use only. Not for human or drug use.
