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Anti-WNT3A Polyclonal Antibody

Catalog #:   PHF18601 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P56704
Overview

Catalog No.

PHF18601

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WNT3A (Ser19-Lys352).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Protein Wnt-3a,WNT3A

Purification

Purified by antigen affinity column.

Accession

P56704

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with WNT3A antibody (PHF18601) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 40 kDa
    Observed MW: 40 kDa
References

Engineered E. coli OMVs Carrying the Membrane-Binding hGC33 Fragment Precisely Target Liver Cancer and Effectively Treat Tumor., PMID:40433120

KIF26B promotes bladder cancer progression via activating Wnt/β-catenin signaling in a TRAF2-dependent pathway., PMID:40253697

Non-Canonical Wnt16 and microRNA-145 Mediate the Response of Human Bone Marrow Stromal Cells to Additively Manufactured Porous 3-Dimensional Biomimetic Titanium-Aluminum-Vanadium Constructs., PMID:39937002

Asiatic acid methyl ester, a new asiaticoside derivative, induces osteogenic differentiation of hPDLCs., PMID:39813772

Serum Wnt3A levels are significantly associated with cross-sectional vasculitis activity and end-stage kidney disease during follow-up of patients with antineutrophil cytoplasmic antibody-associated vasculitis., PMID:39436730

A mechanism of action-reflective, dual cell-based bioassay for determining the bioactivity of sclerostin-neutralizing antibodies., PMID:39389544

O-GlcNAcylation mediates Wnt-stimulated bone formation by rewiring aerobic glycolysis., PMID:39256595

A novel drug prejudice scaffold-imidazopyridine-conjugate can promote cell death in a colorectal cancer model by binding to β-catenin and suppressing the Wnt signaling pathway., PMID:39067696

Synovial osteoclastogenesis mediated by chondrocyte-secreted TNFα promotes TMJ condylar resorption., PMID:38720613

Vitamin D improves autoimmune diseases by inhibiting Wnt signaling pathway., PMID:38414312

NR5A2 promotes malignancy progression and mediates the effect of cisplatin in cutaneous squamous cell carcinoma., PMID:38358044

Carnosic acid nanocluster-based framework combined with PD-1 inhibitors impeded tumorigenesis and enhanced immunotherapy in hepatocellular carcinoma., PMID:38182693

Wnt1 induces osteoblastic changes in a well-established osteolytic skeletal metastatic model derived from breast cancer., PMID:37840014

Wnt/β-catenin pathway is a key signaling pathway to trastuzumab resistance in gastric cancer cells., PMID:37773114

Ehrlichia Wnt SLiM ligand mimic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axis., PMID:37530530

Significance of Lipopolysaccharides in Gastric Cancer and Their Potential as a Biomarker for Nivolumab Sensitivity., PMID:37511547

Proline is increased in allergic asthma and promotes airway remodeling., PMID:37432745

Wnt Signaling Activation in Gingival Epithelial Cells and Macrophages of Experimental Periodontitis., PMID:37232780

Ehrlichia Wnt short linear motif ligand mimetic deactivates the Hippo pathway to engage the anti-apoptotic Yap-GLUT1-BCL-xL axis., PMID:36945589

Sclerostin blockade promotes bone metastases of Wnt-responsive breast cancer cells., PMID:36840409

Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells., PMID:36765434

Interplay Between Chemotherapy-Activated Cancer Associated Fibroblasts and Cancer Initiating Cells Expressing CD44v6 Promotes Colon Cancer Resistance., PMID:35982950

Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer., PMID:35810788

Wnt3a but not CDX-2 expression is associated with differentiated thyroid cancer., PMID:35442371

Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues., PMID:35237403

WNT/beta-catenin signalling interrupts a senescence-induction cascade in human mesenchymal stem cells that restricts their expansion., PMID:35048158

Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling., PMID:35030297

WNT/β-Catenin Signaling Promotes TGF-β-Mediated Activation of Human Cardiac Fibroblasts by Enhancing IL-11 Production., PMID:34576234

Periostin Short Fragment with Exon 17 via Aberrant Alternative Splicing Is Required for Breast Cancer Growth and Metastasis., PMID:33919736

Apoptosis is a generator of Wnt-dependent regeneration and homeostatic cell renewal in the ascidian Ciona., PMID:33913473

Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells., PMID:33331818

The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells., PMID:33276531

Wnt Signaling Mediates Pro-Fibrogenic Activity in Human Aortic Valve Interstitial Cells., PMID:33189669

Neutral Ceramidase Mediates Nonalcoholic Steatohepatitis by Regulating Monounsaturated Fatty Acids and Gut IgA+ B Cells., PMID:33185911

Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells., PMID:32940424

Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo., PMID:32818433

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling., PMID:32762848

Local Wnt3a treatment restores bone regeneration in large osseous defects after surgical debridement of osteomyelitis., PMID:32424558

The Wnt3a/β-catenin/TCF7L2 signaling axis reduces the sensitivity of HER2-positive epithelial ovarian cancer to trastuzumab., PMID:32248976

One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway., PMID:32119023

Regulation of mesenchymal stem cell differentiation on microstructured titanium surfaces by semaphorin 3A., PMID:32028017

Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development., PMID:31985756

WNT signaling inducing activity in ascites predicts poor outcome in ovarian cancer., PMID:31903136

WT1 influences apoptosis and proliferation of immature mice granular cells through regulation of the wnt/β-catenin signal pathway., PMID:31880532

Pre-aggregation of scalp progenitor dermal and epidermal stem cells activates the WNT pathway and promotes hair follicle formation in in vitro and in vivo systems., PMID:31856904

Myometrial Cells Stimulate Self-Renewal of Endometrial Mesenchymal Stem-Like Cells Through WNT5A/β-Catenin Signaling., PMID:31414525

The metastasis suppressor CD82/KAI1 represses the TGF-β 1 and Wnt signalings inducing epithelial-to-mesenchymal transition linked to invasiveness of prostate cancer cells., PMID:31212375

HTRA1 synergizes with oxidized phospholipids in promoting inflammation and macrophage infiltration essential for ocular VEGF expression., PMID:31100080

A Frizzled-Like Cysteine-Rich Domain in Glypican-3 Mediates Wnt Binding and Regulates Hepatocellular Carcinoma Tumor Growth in Mice., PMID:30963603

Targeting IL-17 attenuates hypoxia-induced pulmonary hypertension through downregulation of β-catenin., PMID:30777899

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-WNT3A Polyclonal Antibody [PHF18601]
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