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Anti-ADK Polyclonal Antibody

Catalog #:   PHF11301 Specific References (39) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P55263
Overview

Catalog No.

PHF11301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ADK (Ile94-Ala339).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Adenosine 5'-phosphotransferase,ADK,AK,Adenosine kinase

Purification

Purified by antigen affinity column.

Accession

P55263

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ADK antibody (PHF11301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
  • Immunocytochemistry/ Immunofluorescence
    ADK in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ADK (PHF11301) at 8.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Binding mechanism of adenylate kinase-specific monobodies., PMID:40400134

Cytomegalovirus Antibodies and Coronary Artery Disease in People with HIV: A Cohort Study., PMID:40006986

Cross-sectional and longitudinal genotype to phenotype surveillance of SARS-CoV-2 variants over the first four years of the COVID-19 pandemic., PMID:39549677

A study on viruses and bacteria with particular interest on Mycoplasma pneumoniae in children with exacerbation of asthma from a tertiary care hospital in Sri Lanka., PMID:39081780

Society for Immunotherapy of Cancer (SITC) recommendations on intratumoral immunotherapy clinical trials (IICT): from premalignant to metastatic disease., PMID:38641350

Introducing adjuvant-loaded particulate hepatitis B core antigen as an alternative therapeutic hepatitis B vaccine component., PMID:38425450

Relationship between the Gene Expression of Adenosine Kinase Isoforms and the Expression of CD39 and CD73 Ectonucleotidases in Colorectal Cancer., PMID:37538807

Novel siRNA therapeutics demonstrate multi-variant efficacy against SARS-CoV-2., PMID:37478918

Addition of trastuzumab to perioperative chemotherapy in HER2-positive gastroesophageal adenocarcinoma patients: A multicenter retrospective observational AGEO study., PMID:37407316

Benralizumab for eosinophilic gastritis: a single-site, randomised, double-blind, placebo-controlled, phase 2 trial., PMID:37336228

Serological Evidence of Hepatitis B and E and Dengue Coinfection in Chadian Patients and Impact on Lipidemia Profile., PMID:36158230

SARS-CoV-2 Omicron BA.5: Evolving tropism and evasion of potent humoral responses and resistance to clinical immunotherapeutics relative to viral variants of concern., PMID:36130476

Enhanced immune activation within the tumor microenvironment and circulation of female high-risk melanoma patients and improved survival with adjuvant CTLA4 blockade compared to males., PMID:35659704

Adenosine-Metabolizing Enzymes, Adenosine Kinase and Adenosine Deaminase, in Cancer., PMID:35327609

A hormone complex of FABP4 and nucleoside kinases regulates islet function., PMID:34880500

Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells., PMID:33768128

Pro-inflammatory dopamine-2 receptor-specific T cells in paediatric movement and psychiatric disorders., PMID:33425355

Anti-Siglec-8 Antibody for Eosinophilic Gastritis and Duodenitis., PMID:33085861

Immunogenicity and protective response induced by recombinant Brucella abortus proteins Adk, SecB and combination of these two recombinant proteins against a virulent strain B. abortus 544 infection in BALB/c mice., PMID:32169487

Benralizumab for PDGFRA-Negative Hypereosinophilic Syndrome., PMID:30943337

Sialic acid-binding immunoglobulin-like lectin (Siglec) 8 in patients with eosinophilic disorders: Receptor expression and targeting using chimeric antibodies., PMID:30543818

Opposing effects of intracellular versus extracellular adenine nucleotides on autophagy: implications for β-cell function., PMID:30002135

Chemotherapy-induced pain is promoted by enhanced spinal adenosine kinase levels through astrocyte-dependent mechanisms., PMID:29419652

Early barriers to neonatal porcine islet engraftment in a dual transplant model., PMID:29178588

Genetic Disruption of Adenosine Kinase in Mouse Pancreatic β-Cells Protects Against High-Fat Diet-Induced Glucose Intolerance., PMID:28468960

Impact of Allogeneic Hematopoietic Stem Cell Transplantation on the HIV Reservoir and Immune Response in 3 HIV-Infected Individuals., PMID:28350553

Adenylate kinase: a novel antigen for immunodiagnosis and subunit vaccine against tuberculosis., PMID:26903285

Assessing humoral immune response of 4 recombinant antigens for serodiagnosis of tuberculosis., PMID:25459162

Combined HER2 analysis of biopsies and surgical specimens to optimize detection of trastuzumab-eligible patients in eso-gastric adenocarcinoma: a GERCOR study., PMID:24114855

The consequences of not having eosinophils., PMID:23742015

Enhancement of intestinal immune function in mice by β-D-glucan from aureobasidium pullulans ADK-34., PMID:23672535

Subcellular localization of adenosine kinase in mammalian cells: The long isoform of AdK is localized in the nucleus., PMID:19635462

Development of AAV serotype-specific ELISAs using novel monoclonal antibodies., PMID:17126418

Deficiency of adenosine kinase activity affects the degree of pectin methyl-esterification in cell walls of Arabidopsis thaliana., PMID:16761133

Engineering embryonic stem cell derived glia for adenosine delivery., PMID:15488315

Biochemical properties of rice adenylate kinase and subcellular location in plant cells., PMID:7766884

[Delayed hypersensitivity and nonspecific cellular immunity. The cytotoxic activity of macrophages, natural and antibody-dependent killer cells]., PMID:3087122

Enhancement of tumour growth by pretreatment with tumoral cells adsorbed by poly i:c deae-dextran complex in syngeneic mice., PMID:1035870

[Effect of the cell dose on the development of 2 transplantable tumors in a syngeneic host]., PMID:15919

Datasheet

Document Download

Anti-ADK Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-ADK Polyclonal Antibody [PHF11301]
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