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Anti-MANF Polyclonal Antibody

Catalog #:   PHF10001 Specific References (17) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P55145
Overview

Catalog No.

PHF10001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MANF (Gly28-Leu182).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ARP,ARMET,MANF,Mesencephalic astrocyte-derived neurotrophic factor,Arginine-rich protein,Protein ARMET

Purification

Purified by antigen affinity column.

Accession

P55145

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MANF antibody (PHF10001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with MANF antibody (PHF10001) at 1 μg/ml.

    Lane 1: HEK293

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat spleen stained for MANF with PHF10001.
References

Early Immunotherapy and Longer Corticosteroid Treatment Are Associated With Lower Risk of Relapsing Disease Course in Pediatric MOGAD., PMID:36446614

Hormonal therapies up-regulate MANF and overcome female susceptibility to immune checkpoint inhibitor myocarditis., PMID:36322628

Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs., PMID:34889988

Analysis of Mesencephalic Astrocyte-derived Neurotrophic Factor in Multiple Myeloma., PMID:34475050

Neurotrophic Factors in Parkinson's Disease: Clinical Trials, Open Challenges and Nanoparticle-Mediated Delivery to the Brain., PMID:34149364

Increased MANF Expression in the Inferior Temporal Gyrus in Patients With Alzheimer Disease., PMID:33994992

Mesencephalic astrocyte-derived neurotrophic factor is secreted from interferon-γ-activated tumor cells through ER calcium depletion., PMID:33891607

A Study on Serological Reactivity Profile of Different Antigen Preparations with Bancroftian filariasis Human Infection Sera., PMID:32096736

MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke., PMID:29050872

Increased circulating concentrations of mesencephalic astrocyte-derived neurotrophic factor in children with type 1 diabetes., PMID:27356471

ER stress-inducible protein MANF selectively expresses in human spleen., PMID:26429332

Armet is an effector protein mediating aphid-plant interactions., PMID:25678626

Deficiency of IRE1 and PERK signal pathways in systemic lupus erythematosus., PMID:25226532

Induction profile of MANF/ARMET by cerebral ischemia and its implication for neuron protection., PMID:19773801

Discovering novel phenotype-selective neurotrophic factors to treat neurodegenerative diseases., PMID:14699964

Structural investigation of C4b-binding protein by molecular modeling: localization of putative binding sites., PMID:9626699

Ig-binding surface proteins of Streptococcus pyogenes also bind human C4b-binding protein (C4BP), a regulatory component of the complement system., PMID:7995956

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-MANF Polyclonal Antibody [PHF10001]
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