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Anti-MANF Polyclonal Antibody

Catalog #:   PHF10001 Specific References (17) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P55145
Overview

Catalog No.

PHF10001

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MANF (Gly28-Leu182).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ARP,ARMET,MANF,Mesencephalic astrocyte-derived neurotrophic factor,Arginine-rich protein,Protein ARMET

Purification

Purified by antigen affinity column.

Accession

P55145

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MANF antibody (PHF10001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with MANF antibody (PHF10001) at 1 μg/ml.

    Lane 1: HEK293

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat spleen stained for MANF with PHF10001.
References

Ig-binding surface proteins of Streptococcus pyogenes also bind human C4b-binding protein (C4BP), a regulatory component of the complement system., PMID:7995956

Structural investigation of C4b-binding protein by molecular modeling: localization of putative binding sites., PMID:9626699

Discovering novel phenotype-selective neurotrophic factors to treat neurodegenerative diseases., PMID:14699964

Induction profile of MANF/ARMET by cerebral ischemia and its implication for neuron protection., PMID:19773801

Deficiency of IRE1 and PERK signal pathways in systemic lupus erythematosus., PMID:25226532

Armet is an effector protein mediating aphid-plant interactions., PMID:25678626

ER stress-inducible protein MANF selectively expresses in human spleen., PMID:26429332

Increased circulating concentrations of mesencephalic astrocyte-derived neurotrophic factor in children with type 1 diabetes., PMID:27356471

MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke., PMID:29050872

A Study on Serological Reactivity Profile of Different Antigen Preparations with Bancroftian filariasis Human Infection Sera., PMID:32096736

Mesencephalic astrocyte-derived neurotrophic factor is secreted from interferon-γ-activated tumor cells through ER calcium depletion., PMID:33891607

Increased MANF Expression in the Inferior Temporal Gyrus in Patients With Alzheimer Disease., PMID:33994992

Neurotrophic Factors in Parkinson's Disease: Clinical Trials, Open Challenges and Nanoparticle-Mediated Delivery to the Brain., PMID:34149364

Analysis of Mesencephalic Astrocyte-derived Neurotrophic Factor in Multiple Myeloma., PMID:34475050

Infection and protection responses of deletion mutants of non-structural proteins of foot-and-mouth disease virus serotype Asia1 in guinea pigs., PMID:34889988

Hormonal therapies up-regulate MANF and overcome female susceptibility to immune checkpoint inhibitor myocarditis., PMID:36322628

Early Immunotherapy and Longer Corticosteroid Treatment Are Associated With Lower Risk of Relapsing Disease Course in Pediatric MOGAD., PMID:36446614

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-MANF Polyclonal Antibody [PHF10001]
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