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Anti-Human E2F4 Polyclonal Antibody

Catalog #:   PHH25101 Specific References (29) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q16254
Overview

Catalog No.

PHH25101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human E2F4 (Ser16-Lys82).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

E2F4, Transcription factor E2F4, E2F-4

Purification

Purified by antigen affinity column.

Accession

Q16254

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with E2F4 antibody (PHH25101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 36 kDa
References

Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice., PMID:30936178

The Immunome of Colon Cancer: Functional In Silico Analysis of Antigenic Proteins Deduced from IgG Microarray Profiling., PMID:29505855

Molecular cloning, expression, and characterization of E2F transcription factor 4 from Antheraea pernyi., PMID:28436337

E2F8 as a Novel Therapeutic Target for Lung Cancer., PMID:26089541

PDT-induced epigenetic changes in the mouse cerebral cortex: a protein microarray study., PMID:24055374

Development of ultra-super sensitive immunohistochemistry and its application to the etiological study of adult T-cell leukemia/lymphoma., PMID:22685351

[The cell cycle regulator p130 and beta-catenin form a complex in mesenchymal stem cells]., PMID:21516817

Roles of the ERK, JNK/AP-1/cyclin D1-CDK4 pathway in silica-induced cell cycle changes in human embryo lung fibroblast cells., PMID:21314641

DLK1: increased expression in gliomas and associated with oncogenic activities., PMID:16288219

E2F-HDAC complexes negatively regulate the tumor suppressor gene ARHI in breast cancer., PMID:16158053

Regulation of transcription of the human MRP7 gene. Characteristics of the basal promoter and identification of tumor-derived transcripts encoding additional 5' end heterogeneity., PMID:15474296

Glycogen synthase kinase 3 phosphorylates RBL2/p130 during quiescence., PMID:15456871

Up-regulation of transcriptional factor E2F1 in papillary and anaplastic thyroid cancers., PMID:15118916

The nucleocytoplasmic shuttling of E2F4 is involved in the regulation of human intestinal epithelial cell proliferation and differentiation., PMID:15040009

Expression of E2F-4 gene in colorectal adenocarcinoma and corresponding covering mucosa: an immunohistochemistry, image analysis, and immunoblot study., PMID:12373148

Retinoblastoma-cyclin-dependent kinase pathway deregulation in vestibular schwannomas., PMID:12352662

An E2F-binding site mediates the activation of the proliferative isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by phosphatidylinositol 3-kinase., PMID:12139485

Isolating human transcription factor targets by coupling chromatin immunoprecipitation and CpG island microarray analysis., PMID:11799066

The chromatin structure of the dual c-myc promoter P1/P2 is regulated by separate elements., PMID:11279041

Mechanisms of suberoylanilide hydroxamic acid inhibition of mammary cell growth., PMID:11250759

Expression patterns of retinoblastoma and E2F family proteins during corneal development., PMID:10752941

Modulation of E2F activity in primary mouse B cells following stimulation via surface IgM and CD40 receptors., PMID:10540350

E2F activity is regulated by cell cycle-dependent changes in subcellular localization., PMID:9372959

Induction of S-phase entry by E2F transcription factors depends on their nuclear localization., PMID:9271426

BRCA1 proteins are transported to the nucleus in the absence of serum and splice variants BRCA1a, BRCA1b are tyrosine phosphoproteins that associate with E2F, cyclins and cyclin dependent kinases., PMID:9244350

Accumulation of E2F-4.DP-1 DNA binding complexes correlates with induction of dhfr gene expression during the G1 to S phase transition., PMID:9020173

E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry., PMID:8657117

Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression., PMID:8622649

E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo., PMID:7958925

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human E2F4 Polyclonal Antibody [PHH25101]
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