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Anti-DGKE Polyclonal Antibody

Catalog #:   PHE91901 Specific References (23) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P52429
Overview

Catalog No.

PHE91901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DGKE (Leu243-Gly502).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

DGKE,Diglyceride kinase epsilon,DAG kinase epsilon,DGK-epsilon,Diacylglycerol kinase epsilon,DAGK5

Purification

Purified by antigen affinity column.

Accession

P52429

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with DGKE antibody (PHE91901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
References

Pregnancy-related Thrombotic Microangiopathy has a spectrum of underlying causes., PMID:40306205

Adolescence-onset atypical hemolytic uremic syndrome: is it different from infant-onset?, PMID:38704765

Management of pediatric hemolytic uremic syndrome., PMID:38523374

CFH-CFHR1 hybrid genes in two cases of atypical hemolytic uremic syndrome., PMID:36755127

Variants in complement genes are uncommon in patients with anti-factor H autoantibody-associated atypical hemolytic uremic syndrome., PMID:36622444

Pediatric Atypical Hemolytic Uremic Syndrome Advances., PMID:34944087

Blockade of the Terminal Complement Cascade Using Ravulizumab in a Pediatric Patient With Anti-complement Factor H Autoantibody-Associated aHUS: A Case Report and Literature Review., PMID:34912617

A patient with a homozygous diacylglycerol kinase epsilon (DGKE) gene mutation with atypical haemolytic uraemic syndrome and low C3 responded well to eculizumab: a case report., PMID:33879077

Atypical hemolytic uremic syndrome due to DGKE mutation and response to eculizumab: lessons for the clinical nephrologist., PMID:33751496

Molecular basis and outcomes of atypical haemolytic uraemic syndrome in Czech children., PMID:32424742

Eculizumab treatment of thrombotic microangiopathy in a patient with ulcerative colitis., PMID:31612381

Treatment of hemolytic uremic syndrome related to Bordetella pertussis infection -is plasma exchange or eculizumab use necessary?, PMID:30558570

Atypical presentation of atypical haemolytic uraemic syndrome., PMID:29440240

Glucose-6-Phosphate Dehydrogenase Deficiency Mimicking Atypical Hemolytic Uremic Syndrome., PMID:29248304

Targeted exome sequencing in anti-factor H antibody negative HUS reveals multiple variations., PMID:28939980

Testing the Activity of Complement Convertases in Serum/Plasma for Diagnosis of C4NeF-Mediated C3 Glomerulonephritis., PMID:27146825

[Genetics of aHUS and transplant recurrence]., PMID:26479051

Atypical haemolytic uraemic syndrome in a Japanese patient with DGKE genetic mutations., PMID:26018111

[Atypical HUS caused by complement-related abnormalities]., PMID:25765799

Complement mutations in diacylglycerol kinase-ε-associated atypical hemolytic uremic syndrome., PMID:25135762

Complement therapy in atypical haemolytic uraemic syndrome (aHUS)., PMID:23810412

Complement activation in diseases presenting with thrombotic microangiopathy., PMID:23743117

[Pathophysiology of atypical hemolytic uremic syndrome. Ten years of progress, from laboratory to patient]., PMID:24594571

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-DGKE Polyclonal Antibody [PHE91901]
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