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Anti-VAMP7 Polyclonal Antibody

Catalog #:   PHE87701 Specific References (27) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P51809
Overview

Catalog No.

PHE87701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human VAMP7 (Met1-Met140).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Synaptobrevin-like protein 1,Ti-VAMP,Vesicle-associated membrane protein 7,Tetanus-insensitive VAMP,SYBL1,VAMP7,VAMP-7

Purification

Purified by antigen affinity column.

Accession

P51809

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with VAMP7 antibody (PHE87701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
References

Typhoid toxin sorting and exocytic transport from Salmonella Typhi-infected cells., PMID:35579416

Protocol to study starvation-induced autophagy in developing rat neurons., PMID:34401779

HPS1 Regulates the Maturation of Large Dense Core Vesicles and Lysozyme Secretion in Paneth Cells., PMID:33224134

Dynamic palmitoylation events following T-cell receptor signaling., PMID:32651440

Multiplexed single-cell morphometry for hematopathology diagnostics., PMID:32161403

Comparative study of commercially available and homemade anti-VAMP7 antibodies using CRISPR/Cas9-depleted HeLa cells and VAMP7 knockout mice., PMID:30815249

Human Platelet Vesicles Exhibit Distinct Size and Proteome., PMID:28587468

UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs., PMID:24550300

SNAP-23 and syntaxin-3 are required for chemokine release by mature human mast cells., PMID:21981832

Alternative splicing of the human gene SYBL1 modulates protein domain architecture of Longin VAMP7/TI-VAMP, showing both non-SNARE and synaptobrevin-like isoforms., PMID:21609427

A novel multiprotein complex is required to generate the prechylomicron transport vesicle from intestinal ER., PMID:20237389

Tumor protein D52 expression and Ca2+-dependent phosphorylation modulates lysosomal membrane protein trafficking to the plasma membrane., PMID:20032513

Role of HRB in clathrin-dependent endocytosis., PMID:18819912

The regulated exocytosis of enlargeosomes is mediated by a SNARE machinery that includes VAMP4., PMID:18713833

Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells., PMID:18253931

Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis., PMID:17056503

The identification of a novel endoplasmic reticulum to Golgi SNARE complex used by the prechylomicron transport vesicle., PMID:16735505

A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils., PMID:16677249

Vesicle-associated membrane protein 7 is expressed in intestinal ER., PMID:16495485

Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events., PMID:15133481

Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum., PMID:12175335

Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells., PMID:11278762

[Molecular mechanism of exocytosis in neural and immune system]., PMID:11193390

Syntaxin 7 and VAMP-7 are soluble N-ethylmaleimide-sensitive factor attachment protein receptors required for late endosome-lysosome and homotypic lysosome fusion in alveolar macrophages., PMID:10888671

Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking., PMID:10564279

VAMP-7 mediates vesicular transport from endosomes to lysosomes., PMID:10459012

Raft association of SNAP receptors acting in apical trafficking in Madin-Darby canine kidney cells., PMID:10097106

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-VAMP7 Polyclonal Antibody [PHE87701]
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