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Anti-Human COL4A4 Polyclonal Antibody

Catalog #:   PHE98101 Specific References (18) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P53420
Overview

Catalog No.

PHE98101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human COL4A4 (Val1469-Ser1690).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Collagen alpha-4(IV) chain, COL4A4

Purification

Purified by antigen affinity column.

Accession

P53420

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with COL4A4 antibody (PHE98101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
References

Toripalimab Plus Chemotherapy as a First-Line Therapy for Extensive-Stage Small Cell Lung Cancer: The Phase 3 EXTENTORCH Randomized Clinical Trial., PMID:39541202

Denovo glomerulonephritis associated with IgA anti-GBM alloantibodies after kidney transplantation in Alport syndrome: A case report with diagnostic implications., PMID:39237079

X-linked Alport syndrome presenting in mother and son with the same unique histopathological features., PMID:38668984

Ultrastructural and immunofluorescence analysis of anterior lens capsules in autosomal recessive Alport syndrome., PMID:33251924

Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy., PMID:31630546

Macroscopic hematuria with normal renal biopsy-following the chain to the diagnosis: Answers., PMID:26628280

Keap1 inhibition attenuates glomerulosclerosis., PMID:24523358

Alport alloantibodies but not Goodpasture autoantibodies induce murine glomerulonephritis: protection by quinary crosslinks locking cryptic α3(IV) collagen autoepitopes in vivo., PMID:20709951

Ultrastructural defects of the glomerular basement membranes associated with primary glomerular nephropathies., PMID:15205110

A human-mouse chimera of the alpha3alpha4alpha5(IV) collagen protomer rescues the renal phenotype in Col4a3-/- Alport mice., PMID:14507670

Quantitative analysis of glomerular type IV collagen alpha3-5 chain expression in children with thin basement membrane disease., PMID:12218303

Histopathology, ultrastructure, and clinical phenotypes in thin glomerular basement membrane disease variants., PMID:12203217

Identification of COL4A5 defects in Alport's syndrome by immunohistochemistry of skin., PMID:10200983

Autosomal recessive Alport syndrome: linkage analysis and clinical features in two families., PMID:10193810

Ultrastructural, physiological, and molecular defects in the inner ear of a gene-knockout mouse model for autosomal Alport syndrome., PMID:9682811

Type IV collagen in sponges, the missing link in basement membrane ubiquity., PMID:9175266

A COL4A3 gene mutation and post-transplant anti-alpha 3(IV) collagen alloantibodies in Alport syndrome., PMID:7783419

Autosomal recessive Alport syndrome: immunohistochemical study of type IV collagen chain distribution., PMID:7783412

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human COL4A4 Polyclonal Antibody [PHE98101]
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