Catalog No.
PHE98101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human COL4A4 (Val1469-Ser1690).
Applications
ELISA, IF, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Collagen alpha-4(IV) chain, COL4A4
Purification
Purified by antigen affinity column.
Accession
P53420
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Autosomal recessive Alport syndrome: immunohistochemical study of type IV collagen chain distribution., PMID:7783412
A COL4A3 gene mutation and post-transplant anti-alpha 3(IV) collagen alloantibodies in Alport syndrome., PMID:7783419
Type IV collagen in sponges, the missing link in basement membrane ubiquity., PMID:9175266
Ultrastructural, physiological, and molecular defects in the inner ear of a gene-knockout mouse model for autosomal Alport syndrome., PMID:9682811
Autosomal recessive Alport syndrome: linkage analysis and clinical features in two families., PMID:10193810
Identification of COL4A5 defects in Alport's syndrome by immunohistochemistry of skin., PMID:10200983
Histopathology, ultrastructure, and clinical phenotypes in thin glomerular basement membrane disease variants., PMID:12203217
Quantitative analysis of glomerular type IV collagen alpha3-5 chain expression in children with thin basement membrane disease., PMID:12218303
A human-mouse chimera of the alpha3alpha4alpha5(IV) collagen protomer rescues the renal phenotype in Col4a3-/- Alport mice., PMID:14507670
Ultrastructural defects of the glomerular basement membranes associated with primary glomerular nephropathies., PMID:15205110
Alport alloantibodies but not Goodpasture autoantibodies induce murine glomerulonephritis: protection by quinary crosslinks locking cryptic α3(IV) collagen autoepitopes in vivo., PMID:20709951
Keap1 inhibition attenuates glomerulosclerosis., PMID:24523358
Macroscopic hematuria with normal renal biopsy-following the chain to the diagnosis: Answers., PMID:26628280
Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy., PMID:31630546
Ultrastructural and immunofluorescence analysis of anterior lens capsules in autosomal recessive Alport syndrome., PMID:33251924
X-linked Alport syndrome presenting in mother and son with the same unique histopathological features., PMID:38668984
Denovo glomerulonephritis associated with IgA anti-GBM alloantibodies after kidney transplantation in Alport syndrome: A case report with diagnostic implications., PMID:39237079
Toripalimab Plus Chemotherapy as a First-Line Therapy for Extensive-Stage Small Cell Lung Cancer: The Phase 3 EXTENTORCH Randomized Clinical Trial., PMID:39541202
For research use only. Not for human or drug use.
