Catalog No.
PHE10601
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PTGER4/EP4 Receptor (Asn155-Ser185).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
PTGER2, Prostaglandin E2 receptor EP4 subtype, PTGER4, PGE receptor EP4 subtype, Prostanoid EP4 receptor, PGE2 receptor EP4 subtype
Purification
Purified by antigen affinity column.
Accession
P35408
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
A conserved threonine in the second extracellular loop of the human EP2 and EP4 receptors is required for ligand binding., PMID:9788776
Immunolocalization of the four prostaglandin E2 receptor proteins EP1, EP2, EP3, and EP4 in human kidney., PMID:10477136
Comparison of agonist-induced internalization of the human EP2 and EP4 prostaglandin receptors: role of the carboxyl terminus in EP4 receptor sequestration., PMID:11093764
Prostaglandin E(2) regulates the level and stability of cyclooxygenase-2 mRNA through activation of p38 mitogen-activated protein kinase in interleukin-1 beta-treated human synovial fibroblasts., PMID:11423555
Sequestration and phosphorylation of the prostaglandin E2 EP4 receptor: dependence on the C-terminal tail., PMID:11597569
Expression and localization of FP and EP prostanoid receptor subtypes in human ocular tissues., PMID:11980863
The role of prostaglandin E2 receptors in the pathogenesis of rheumatoid arthritis., PMID:12208866
Interleukin-1beta-induced mucin production in human airway epithelium is mediated by cyclooxygenase-2, prostaglandin E2 receptors, and cyclic AMP-protein kinase A signaling., PMID:15266025
Activation of EP4 receptors contributes to prostaglandin E2-mediated stimulation of renal sensory nerves., PMID:15292051
Prostaglandin E2 receptor subtypes, EP1, EP2, EP3 and EP4 in human and mouse ocular tissues--a comparative immunohistochemical study., PMID:15380814
Role of prostaglandin E2 receptor subtypes in ovarian follicle growth in the rat in vivo. Correlation with interleukin-8 and neutrophils., PMID:15944932
A novel prostaglandin E receptor 4-associated protein participates in antiinflammatory signaling., PMID:16424369
Prostacyclin-IP signaling and prostaglandin E2-EP2/EP4 signaling both mediate joint inflammation in mouse collagen-induced arthritis., PMID:16446378
Protective effect of prostaglandin EP4-receptor agonist on anti-glomerular basement membrane antibody-associated nephritis., PMID:17031072
Extracellular matrix fibronectin increases prostaglandin E2 receptor subtype EP4 in lung carcinoma cells through multiple signaling pathways: the role of AP-2., PMID:17237224
8-iso-PGE2 stimulates anion efflux from airway epithelial cells via the EP4 prostanoid receptor., PMID:17690331
MF498 [N-{[4-(5,9-Diethoxy-6-oxo-6,8-dihydro-7H-pyrrolo[3,4-g]quinolin-7-yl)-3-methylbenzyl]sulfonyl}-2-(2-methoxyphenyl)acetamide], a selective E prostanoid receptor 4 antagonist, relieves joint inflammation and pain in rodent models of rheumatoid and osteoarthritis., PMID:18287210
Proneoplastic effects of PGE2 mediated by EP4 receptor in colorectal cancer., PMID:19558693
A novel antagonist of the prostaglandin E(2) EP(4) receptor inhibits Th1 differentiation and Th17 expansion and is orally active in arthritis models., PMID:20423341
Prostaglandin E2-prostaglandin E receptor subtype 4 (EP4) signaling mediates UV irradiation-induced systemic immunosuppression., PMID:21460251
Fluid flow shear stress upregulates prostanoid receptor EP2 but not EP4 in murine podocytes., PMID:23262148
Autocrine and paracrine mechanisms of prostaglandin E₂ action on trophoblast/conceptus cells through the prostaglandin E₂ receptor (PTGER2) during implantation., PMID:23861370
Prostaglandin E2-EP4 signaling persistently amplifies CD40-mediated induction of IL-23 p19 expression through canonical and non-canonical NF-κB pathways., PMID:26189370
Prostaglandin E2 Activates YAP and a Positive-Signaling Loop to Promote Colon Regeneration After Colitis but Also Carcinogenesis in Mice., PMID:27864128
Genomic and transcriptomic comparison of allergen and silver nanoparticle-induced mast cell degranulation reveals novel non-immunoglobulin E mediated mechanisms., PMID:29566008
Role of S1P/S1PR3 axis in release of CCL20 from human bronchial epithelial cells., PMID:30192865
Ligand binding to human prostaglandin E receptor EP4 at the lipid-bilayer interface., PMID:30510193
Single-Cell Analysis Reveals EP4 as a Target for Restoring T-Cell Infiltration and Sensitizing Prostate Cancer to Immunotherapy., PMID:34740924
Blockade of EP4 by ASP7657 Modulates Myeloid Cell Differentiation In Vivo and Enhances the Antitumor Effect of Radiotherapy., PMID:38058393
The Prostaglandin EP4 Antagonist Vorbipiprant Combined with PD-1 Blockade for Refractory Microsatellite-Stable Metastatic Colorectal Cancer: A Phase Ib/IIa Trial., PMID:39620921
For research use only. Not for human or drug use.
